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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Camera93]
    #26408408 -

@Amanita;
Both PESH plates look good as hell. Both look ready for grain IMO.
Personally, the first GT plate is 1-2 transfers away from being ready for grain, the second GT could need 2-3 transfers, using that 5:00 - 8:00 spot.
About the first two RW plates, is the myc aerial in any way? I mean, is it growing up and off the surface, or is it pretty flat against the surface? If it's flat, i'd say it's good to go. Last two RW's are ready, could transfer one more time just for shits.

@AKA;
Those first two do look odd, but only because the growth is unorganized and nonuniform. Same with the last plate, it's just unorganized and non uniform. All three look pretty clean, IMO, but i'd transfer a couple more times to get them more uniform. With the first two, try to grab that super ropey growth, that will make for a dope lookin plate!

About the condensation, if your sterile tek is good, that shouldn't be a problem! It is adding another vector of contamination, but follow proper procedure and you'll be fine.

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: MLPismyOPSEC]
    #26408460 -

MLPismyOPSEC said:
Both PESH plates look good as hell. Both look ready for grain IMO.
Personally, the first GT plate is 1-2 transfers away from being ready for grain, the second GT could need 2-3 transfers, using that 5:00 - 8:00 spot.
About the first two RW plates, is the myc aerial in any way? I mean, is it growing up and off the surface, or is it pretty flat against the surface? If it's flat, i'd say it's good to go. Last two RW's are ready, could transfer one more time just for shits.



Cool, thank you very much! I just took another look at the tomentose RW plates and don't think they're very aerial. The mycelium puffs up a bit, but no higher than the original agar wedge.


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My LAGM 2020 Journal

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: nevereataamanita]
    #26408540 -

Bacterial contamination is the cause of the bite-shaped chunks on this T2 plate of cube myc?


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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: bongoman]
    #26409221 -

Thanks man that last gt clone plate is just so weird to me.

Bongo- thatโ€™s prob bacteria. I donโ€™t think Iโ€™ve ever seen a pic of such thick myc with clear cut chunks like that.


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LAGM2020

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: A.k.a]
    #26409513 -

You guys use vented or nonvented plates?

I used vented last time but I wrap the shit out of them anyways so does it even matter?

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: SpunkyMonkey88]
    #26409524 -

I dnt thnk it matters, but i use vented outta habit.

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Feasoghorm]
    #26410531 -

What's up everyone...so Ive finally taken the dive into agar. Been growing off and on for 20 years and usually had great success with MS grows so I never took the leap. Well this past year Ive ran into quite a bit of contams for some reson. Ive never had this many issues before. Anyway check these plates out. Its my second attempt. Im still learning what is myc and what isnt. Are these all worthy of transferring? Btw, sorry for the n00b mistake of labeling them in the middle of the plate, lol!


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Man says, "God, show me and I will believe." God says, "Believe and I will show you."

Edited by Aiko Aiko (01/01/20 07:50 AM)

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Aiko Aiko]
    #26410613 -

Iโ€™d go for between 9-11 on the first plate. The other two had probs with the picture.


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LAGM2020

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: A.k.a]
    #26410621 -



Not sure whats going on with the pics, I think it's on their end.


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Man says, "God, show me and I will believe." God says, "Believe and I will show you."

Edited by Aiko Aiko (01/01/20 08:57 AM)

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Aiko Aiko]
    #26410808 -

I have a question about running a culture long term.

Say you take a tissue sample and end up using it as a master plate. Normally when making plates to put to grain you transfer from the edge to get faster growth which also would reduce the number of strains that make it to the next plate.

So the point of the master plate is to avoid senescence right? Eventually the plate will start to dry out and youโ€™ll have it make a new one. Would it be best to take a transfer from close to the center of the dish to get as many of the original strains as possible before they sector out??

Does this even make sense? Iโ€™m just getting into agar and learning how mushrooms genetics work.


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LAGM2020

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: A.k.a]
    #26411132 -

Actually if you dnt keep a genetically diverse master in the fridge that you take tissue from now and then, you'll be consistantly narrowing genetics. I've done this.
When i started my PE culture it fruited mad. Last tubs i did with it jst blobed and mutated on an ultra sparse landscape. All of my cultures did that kinda shit eventually.

Imma multispore enthusiast for sure. When i get settled again late summer imma do shit way different. No more 100qt tubs. No more narrowing down genetics. Mother nature knows what she doing.

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: A.k.a]
    #26411141 -

You'll want to store your master culture in a slant, with a 'working' master petri that you replace periodically with a new one cultured from the master slant: https://www.shroomery.org/forums/showflat.php/Number/18182209

I can't speak to where it would be best to sample from on your plate for making that slant, but I would guess still the edge.

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Feasoghorm]
    #26411145 -

Thatโ€™s what I mean. I would think you want to keep as much of the genetics from the original culture as possible, which is why I would think when making a new master plate closer to the middle would be best. But Iโ€™m not even sure it works like that Iโ€™m assuming since as it grows it sectors out that the closer to center you go the more diverse it is.

A slant would be best for sure but this is mostly just theoretical right now to make sure I actually understand how all this works.

The whole agar/genetics thing is a new dimension of cult.


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LAGM2020

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: A.k.a] * 1
    #26411207 -

RW and LUPE plates



VERY narrow gene pools: great performers :shrug:


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๐Ÿ„๐Ÿ’š๐Ÿ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Caps McGee]
    #26411411 -

I thnk possibly the thing about a slant is that there is not much growth, so there is not much shot fanning out to take a little bit of.

Im guessing your correct in thinking that a tissue sample from close to the original tissue sample wud preserve more genetics than sampling from an outter edge.

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Caps McGee]
    #26411418 -

Could I get some pointers with these plates?  First of all, how do they look? Do any look ready for grain? I like the look of plates 1 and 3, they have grown the fastest, too.

1


2


3


4


Also, what are some good ways I can utilize these 2-section petris? I was initially thinking to transfer from one side to the other. I am a bit late with this transfer, but would this generally be a good idea? Any other ways I can take advantage of the 2 sections?


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LAGM 2021
LAGM 2020
Trade List

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: One of Us]
    #26411495 -

Those look good to me.

Iโ€™ve never used the multi compartment dishes but I would either do like you said and use half for say T1 then the other half to move a piece over for t2.

Or maybe take a T1 on each side then pick whatever looks best to expand from there.

The divide goes to the lid right? So if you used one side and got bacteria or something you could take a quick transfer over the wall and not have to use a new dish.


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LAGM2020

Edited by A.k.a (01/01/20 08:12 PM)

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: A.k.a]
    #26411561 -

A.k.a said:
A slant would be best for sure but this is mostly just theoretical right now to make sure I actually understand how all this works.


You do understand it correctly; yes each time you take a section from the edge, you are narrowing genetics. You would be including the largest array of genetics by taking a piece from the center. But how far does that actually get you? I definitely don't have that answer! Post a new thread about this (or post it in here https://www.shroomery.org/forums/showflat.php/Number/25911960/page/1), i've been thinking about this exact topic for a while now, since i'll be considering making masters soon.

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: MLPismyOPSEC]
    #26411575 -

I postulate issues with "genetics" and cultures "wearing down" to be more based in contamination than the dreaded senescence personally... bacteria picked up somewhere or of the sort... I've been running the same blue oyster and lpeu culture by transferring out once and using the rest each round for 40 and 25 respectively... as in, I dont do masters, always transfer from the edge, and typically only see better(more consistent) results with genetic isolation... that said,  I dont waste time digging for diamonds in lumps of coal: if ms doesn't grab my attention,  its not running again


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๐Ÿ„๐Ÿ’š๐Ÿ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All Things Agar!) [Re: Caps McGee]
    #26412171 -

Hey guys so Iโ€™m wondering if this is what bacteria looks like on agar?

Also I think I fucked upnand cooled my scaple right next to it  :facepalm3:


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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