Bobbit said:

LtLurker said:

Clearing up terms. . .

A clone is just a clone, it can contain 1000's of strains.

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Can you point me to any microscopic research that confirms this? I have been led to believe this is not the case and apart from the growers who make assumptions at a macro level, this seems far from intuitive.

Each of the 1000’s of strains would have to be growing at almost exactly the same speed to be maintained after multiple leading edge transfers, which seems particularly unlikely considerring they are all competing for resource at the same time. wouldn’t he who gets the best resource start, take the lead, taking the lions share of the nutrient as progressing, promoting stronger growth?

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LtLurker said:

lol, i almost cringed a sec when you said stamets, but that right there is much better said than how i put it. thanks spirit.

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LtLurker said:

@replyom i mean if you just dripped 4 drops straight onto the plate, i would only expect 4 points of germination at most. Usually people will swipe an innoc loop around with 1 drop to spread out it's contents so you'd see more points along the swipe path with myc, mold or bacteria growing from them.

If you did swipe it, it could just be a very dilute syringe. I have had some swabs that would only germ in a few spots across the entire swiped surface. There's a few possible reasons to see what you're seeing there.

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LtLurker said:

I don't have a science background and there's some other guys that could go into much better details on specific things where i can only give examples. But the gist of it is he's just not a very reliable researcher and most growers on this site are not fans of him. There's a lot of things in his books related to growing that were just assumptions he stated as facts without any real attempts at verifying through experiments. like colonizing in dark as an example, a simple series of tests would have clearly shown that's just not true and more detrimental than benign. Also things like claiming to discover new species that were already known, weird "magic cure" stories with mushrooms, stuff like that.

Then of course there's the overall problem with books not updating. So even a great growing guide will eventually be considered outdated, while a live forum like this will constantly be growing and re-evaluating teks and standars.

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Shroomwomb said:

Well I feel like a dumbass. I posted here a few days ago asking about what I did wrong pouring my plates, as all of them had the same mold and no mycelium growth. Well one thing I didn't mention is that I didn't wash out my media bottle after I finished pouring the plates, I just capped it with a little agar left in the bottom. I just pulled the bottle out and noticed the same mold growing on my plates is growing inside my media bottle. This must mean it wasn't sterilized during my pressure cooking? I don't understand why, I followed the tek, let it steam, reach 15psi, then cook for 20 minutes. Help please.
I have 16 plates that I assume are fucked. Kinda bummed.

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Edmunter said:

Shroomwomb said:

Well I feel like a dumbass. I posted here a few days ago asking about what I did wrong pouring my plates, as all of them had the same mold and no mycelium growth. Well one thing I didn't mention is that I didn't wash out my media bottle after I finished pouring the plates, I just capped it with a little agar left in the bottom. I just pulled the bottle out and noticed the same mold growing on my plates is growing inside my media bottle. This must mean it wasn't sterilized during my pressure cooking? I don't understand why, I followed the tek, let it steam, reach 15psi, then cook for 20 minutes. Help please.
I have 16 plates that I assume are fucked. Kinda bummed.

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What was your method in the sab?

Failures are just helping you fine tune greatness.....

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For the pour I wiped with iso, sprayed with soap water, set the plates and media bottle in. Wiped my forearms and gloves with iso. After taking the lid off the media bottle I started pouring one plate up from the bottom, picking up the lid and the 5orso plates on top. Then I went up the stack. I put the media lid back on after I finished pouring 19 plates (I overpoured a few and ended up one short).
For inoculation I just flamed the needle, too off the lid along with the plates above, dropped on average ~3 drops onto each plate, and inoculated 4.
I just wrapped 6 of the remaining plates from my fridge in the sab and put them on the shelf. If they also grow I will throw the rest of them away and repour.

MH5109 said:

I know clones iv taken have visibly different growth rates on agar, both of these were first transfer of an aa+ clone.

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Tormato said:

MH5109 said:

I know clones iv taken have visibly different growth rates on agar, both of these were first transfer of an aa+ clone.

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Proving that clones are not isolates...IMO

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Edmunter said:

Tormato said:

MH5109 said:

I know clones iv taken have visibly different growth rates on agar, both of these were first transfer of an aa+ clone.

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Proving that clones are not isolates...IMO

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Are there people who think there are?  It definetly easier to get an isolate from a clone but Ididnt think it was a common mistake.  I could be wrong.

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I've just gotten into brawls with people that insisted clones were isolates...I agree it gets you there faster, but not an isolate. Sorry to MH5109...didn't mean to single you out man just using your pictures as reference.