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downforpot
Stranger
Registered: 06/25/01
Posts: 5,715
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using agar?
#477105 - 12/02/01 12:39 PM (22 years, 9 months ago) |
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what is agar? what is it used for? can i use cotaminated syringes to grow healthy mycelium on agar? what do i need to start using agar?
-------------------- http://www.myspace.com/4th25 "And I don't care if he was handcuffed Then shot in his head All I know is dead bodies Can't fuck with me again"
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cantara
member
Registered: 08/06/01
Posts: 133
Loc: Beyond the sun
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Re: using agar? [Re: downforpot]
#477132 - 12/02/01 01:19 PM (22 years, 9 months ago) |
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Agar is a seaweed derivative that is liquid when heated and gels to a jello-like consistency when cooled back to room temp. In mushroom cultivation, the idea is to nutrify the agar with ingredients that are nutritious for mushroom mycelium, essentially mix the ingredients together with water, pressure cook and allow to cool complete. Mycelium culture is then grown on agar to be transferred to spawn/substrate. No, if you have contaminated syringes, you don't want to grow them on agar, the contams will simply thrive on the agar. Here is a basic-info agar link from grow/find of the Shroomery. You can buy agar itself in health food stores, or buy MEA agar from fungiperfecti.com. http://www.shroomery.org/findorgrowthem.php?View=docs&doc=115
-------------------- ---- Cantara [green]Shroomism, please don't delete this thread![/green]
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downforpot
Stranger
Registered: 06/25/01
Posts: 5,715
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Re: using agar? [Re: cantara]
#477370 - 12/02/01 05:19 PM (22 years, 9 months ago) |
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about the petri dishes, im gonna be usin a pressure cooker to sterelize em, so should i buy glass or plastic ones? will the plactic ones survive the 15psi?
-------------------- http://www.myspace.com/4th25 "And I don't care if he was handcuffed Then shot in his head All I know is dead bodies Can't fuck with me again"
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carbonhoots
old hand
Registered: 09/11/01
Posts: 1,351
Loc: BC Canada
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Re: using agar? [Re: downforpot]
#477390 - 12/02/01 05:40 PM (22 years, 9 months ago) |
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petris suck. I use empty jam jars turned on their sides.
-------------------- -I'd rather have a frontal lobotomy than a bottle in front of me CANADIAN CENTER FOR POLICY ALTERNATIVES
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,167
Loc: my room
Last seen: 29 days, 15 hours
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Re: using agar? [Re: downforpot]
#477405 - 12/02/01 05:59 PM (22 years, 9 months ago) |
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The plastic petri dishes come presterilized and are ment for 1 time use. They don?t survive pressure cooking. If you will use agar only accasionally and on small scale, then I recommend to buy 10 glas dishes.(but that?s me, you can off course go plastic independant from the scale...) If you want to make it big style, plastic is the way to go. Buy The Mushroom Cultivator from Paul Stamets, this book has thissubject pretty well covered.
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downforpot
Stranger
Registered: 06/25/01
Posts: 5,715
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Re: using agar? [Re: Anno]
#477471 - 12/02/01 06:47 PM (22 years, 9 months ago) |
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what sites can i get agar from?
-------------------- http://www.myspace.com/4th25 "And I don't care if he was handcuffed Then shot in his head All I know is dead bodies Can't fuck with me again"
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,167
Loc: my room
Last seen: 29 days, 15 hours
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Re: using agar? [Re: downforpot]
#477486 - 12/02/01 07:00 PM (22 years, 9 months ago) |
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www.sporeworks.com www.mycosupply.com www.myceliumfruits.com www.mushmush.nl www.fungi.com www.moorhead-agar.com . . . Oh, and read http://www.mykoweb.com/articles/cultivation.html.
Edited by Anno (12/02/01 07:01 PM)
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