replyom said:

Thanks a lot Edmunter. I understand and agree with all of that. But as far as contamination goes, the plates look clean and ready to go to grain? I’m just trying to get one or two nice fruits to clone, as quickly as possible, so I’m okay with doing a2g with all of these plates instead of spending a few more weeks doing transfers.

Thanks again!

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As said, id go one more transfer on said plates and take a chance with 1-5. 

This is a clean plate


Its not like the growth id like to use for a grow yet.  Maybe someone else can chime in here.

Cthulhu23 said:

Got some LME at a brewing store today, and will be making some plates tonight.  Usually I make my Agar from with GSA or BRF so this’ll be a first for me. 

Planning on 2% for both the LME and agar - thinking of throwing in some food coloring to make spotting contams easier.  Question: what color do you all use (I see a lot of red and blue), and about how many drops for 500ml do you guys use?

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Edmunter said:

replyom said:

Thanks a lot Edmunter. I understand and agree with all of that. But as far as contamination goes, the plates look clean and ready to go to grain? I’m just trying to get one or two nice fruits to clone, as quickly as possible, so I’m okay with doing a2g with all of these plates instead of spending a few more weeks doing transfers.

Thanks again!

---

As said, id go one more transfer on said plates and take a chance with 1-5. 

This is a clean plate


Its not like the growth id like to use for a grow yet.  Maybe someone else can chime in here.

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jbgtaa said:

Edmunter said:

replyom said:

Thanks a lot Edmunter. I understand and agree with all of that. But as far as contamination goes, the plates look clean and ready to go to grain? I’m just trying to get one or two nice fruits to clone, as quickly as possible, so I’m okay with doing a2g with all of these plates instead of spending a few more weeks doing transfers.

Thanks again!

---

As said, id go one more transfer on said plates and take a chance with 1-5. 

This is a clean plate


Its not like the growth id like to use for a grow yet.  Maybe someone else can chime in here.

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Idk what my suggestion means to you, but that's a beautiful plate and if i had a beer that day it would probably go to 4 jars. If i were sober and thinking correctly, only 2

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GypsyCurse said:

Betcha the myc sucked up the color in the agar

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Yep! Mycelium loves food coloring

Edmunter said:

replyom said:

Thanks a lot Edmunter. I understand and agree with all of that. But as far as contamination goes, the plates look clean and ready to go to grain? I’m just trying to get one or two nice fruits to clone, as quickly as possible, so I’m okay with doing a2g with all of these plates instead of spending a few more weeks doing transfers.

Thanks again!

---

As said, id go one more transfer on said plates and take a chance with 1-5. 

This is a clean plate


Its not like the growth id like to use for a grow yet.  Maybe someone else can chime in here.

---

jbgtaa said:

Sorry, i guess you didnt see my previous post...
Those plates look very fluffy and uneven to me. A Trusted Cultivator has told you take transfers. I agree with that. If you must, put the best looking one to grain, probably the 8/9 o clock position on plate number 3. The rest of them need to keep getting cleaned.

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Edmunter said:

replyom said:

I have 5 plates of Argentina variety that should be ready to transfer to grain in a couple of days (this is the third transfer from MS).

Trying to learn more about what to look for on agar. There are no bacterial colonies, so that's good...

Anything I should notice that would prevent me from going to grain?

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The odd shape and resistance to grow in certain area.  If you transfer the healthiest mtc you are taking healthy genetics and losing the slow creepy ones.  If you transfer the fastest growing myc you lose the slow stuff.  If you transfer the pretty myc you lose the ugly myc ect. 
  Would give me concern about being contaminated
Using 1 and 5 gives you room to reduce the genetics in the others.

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So with the above edit above I would use 1 and 5 for grains as they are more uniform and transfer the rest for safety.  This way you are covering your arse   What you want is uniformety and to be able to see the mycellium clearly.  If you look at my 2 cultures above 1 is clearly clean and the other im 95% sure.

You only need 1 clean jar to expand to 100s