newtomyc said:

I'm curious if anyone bothers to pH the Agar? Does it make a difference in growth?


JJ

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Caps McGee said:

And why did you take so much tissue?


Some promising looking, workable cultures you got thur!

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Thanks!  I’m pretty stoked about them.  No bacteria or mold.  I think just about every clone I’ve done in the past has had at least one plate with bacteria right off the bat.  Guess I’m starting to get the hang of things:-p

Note:  I put that disclaimer in just for you Caps

Brain Bulb said:

Recent clones making good progress!  (Can already hear some comments.  “Why did you take so much tissue?”  “I usually take 1/4” at the most”)

My answer - That’s just how it worked out. 

1.) These are mini pin clones I took from this Roatan plate.  (Didn’t save the actual pic.  This one is from about a week before I transferred).



Best looking plate:



2.) Roatan clone #2 - Cloned this fat boy.



CP1 -



CP2 -



3.) AA+ clones taken from this specimen.



CP1 -



CP2 - (**Changed pics to actual AA+ clone)

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Humanhelper said:

If anybody is willing to burn a little time with suggestions today, fire away.  I tested an LC for contams to a couple PF TEK jars I had left over from another project on 1/5/19, birthed the cake in the pic 1/25, and now woke up to decisions to make.  My previous tissue to agar samples did not go well and I think part of it was poor donors and shitty agar pours.  I now have plates that are money, clear as a bell and without the gallons of condensation of my previous disasters.  So, obviously the cluster is the best choice, ya?  Do these fruits express traits worth cloning...to me they look dope, just not super huge.  Which specimen would you take tissue from? And who prefers what, base or cap meat?
B+ from LC to BRF



I don't want to waste plates, and have plenty of other projects, but if these look good enough let me know.  I'll pull 2nd and third flush prints for my library and trades.
HH

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Vibe_Enthusiast said:

Brain Bulb said:

Recent clones making good progress!  (Can already hear some comments.  “Why did you take so much tissue?”  “I usually take 1/4” at the most”)

My answer - That’s just how it worked out. 

1.) These are mini pin clones I took from this Roatan plate.  (Didn’t save the actual pic.  This one is from about a week before I transferred).



Best looking plate:



2.) Roatan clone #2 - Cloned this fat boy.



CP1 -



CP2 -



3.) AA+ clones taken from this specimen.



CP1 -



CP2 - (**Changed pics to actual AA+ clone)

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On the 'best looking plate'... did you drop two tissue samples on that agar?

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Brain Bulb said:

This clone plate is looking so good I might let it incubate for a couple more days and transfer to grain.  Bad idea?

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Caps McGee said:

HH, I'd clone at least the largest of the cluster, just to have a clone culture of the variety on hand... remember that smaller tissue samples will recover more quickly and carry less risk... BB, I've dropped a few tissue plates with success, just depends on how confident you are with your sterile technique when it was taken... good idea to transfer a very small wedge to a new plate for expansion: just in case... alternatively, you could drop and omit the center wedge with the tissue sample on it entirely, just flip it out onto the floor of the SAB with the scalpel prior to A2G

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