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Preferred Agar Recipes for Huatla
    #25484835 -

Trying to find preferred agar recipes for huatla. I tried PDA but for some reason the ms syringe didnt take when i did a swipe (the golden teacher is loving PDA). Will try some LME when i can get to the brew store. I also tried to put a swipe from a wild print of Pan Cinctulus on the PDA with no results...

Right now Im using pastyplates with 500ml water, 10g agar powder + 8g potato flakes + 6g corn syrup

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Re: Preferred Agar Recipes for Huatla [Re: browntonabby]
    #25484852 -

Huatla is a name printed on a label it has absolutely 0, nothing, zilch, to do with the agar recipe you choose.

Cubes work great on PDA.

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Re: Preferred Agar Recipes for Huatla [Re: bodhisatta]
    #25484861 -

:whathesaid:

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Re: Preferred Agar Recipes for Huatla [Re: Munchauzen]
    #25484915 -

Trying to get spores to germinate on agar tends to need a slightly less hard mix, at least in my exp, it's a little more moist etc..as you're currently using 10g, that's gonna be quite hard, so try half of the usual amount of agar i.e 5g and test. You could cut the potato back to 5g too I'd imagine as that's the amount I use with good results

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Re: Preferred Agar Recipes for Huatla [Re: Shroomspective]
    #25484926 -

I use the same mix for everything. 2% agar, 2% malt extract.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Re: Preferred Agar Recipes for Huatla [Re: 36fuckin5]
    #25484945 -

May be different for PDA mixes? I've had zero % spores germinate on 9g agar, 5g Pot, 1g yeast and 10ml Honey, from spore prints or syringes, even though normal transfers love it and cover a plate in a few days flat..Couple of old threads that had good ideas in came to the conclusion that it was the agar stiffness

https://www.shroomery.org/forums/showflat.php/Number/16380326
https://www.shroomery.org/forums/showflat.php/Number/21337631#21337631

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Re: Preferred Agar Recipes for Huatla [Re: Shroomspective]
    #25485171 -

You're adding yeast? It's supposed to be yeast nutrient, not actual yeast.

I've used PDA before and never saw a problem.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Re: Preferred Agar Recipes for Huatla [Re: 36fuckin5]
    #25485202 -

Yup actual yeast, just ground up into a fine powder rather than the tiny round balls....Seems to work as growth is rapid, I've been thinking about giving Vegemite a try to see what happens

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Re: Preferred Agar Recipes for Huatla [Re: Shroomspective]
    #25485268 -

I hate seeing recipes like this in percentages. is it 2% by weight, volume, also with water and powder ingredients it gets confusing. This is what i use which is probably similar.

1 teaspoon agar
2 teaspoons potato flakes
1 drop karo
1/2 cup water
1 drop food coloring

Mix together in a sauce pan on medium. Pour into jars or glad mini rounds and pc.

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Re: Preferred Agar Recipes for Huatla [Re: Shroomspective]
    #25485276 -

Shroomspective said:
Yup actual yeast, just ground up into a fine powder rather than the tiny round balls....Seems to work as growth is rapid, I've been thinking about giving Vegemite a try to see what happens



Vegemite is all salt so I would suggest avoiding it.
Also yeast should be autolyzed yeast extract for agar

scab eater said:
I hate seeing recipes like this in percentages. is it 2% by weight, volume, also with water and powder ingredients it gets confusing. This is what i use which is probably similar.

1 teaspoon agar
2 teaspoons potato flakes
1 drop karo
1/2 cup water
1 drop food coloring

Mix together in a sauce pan on medium. Pour into jars or glad mini rounds and pc.



Spoonful recipes like that are atrocious. Percentages are ideal

"A drop"
:uhno:

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Re: Preferred Agar Recipes for Huatla [Re: bodhisatta]
    #25485290 -

Guess I just suck at math, but percentages confuse me in agar recipes. Maybe it's better for larger amounts. What i posted makes about 8 glad mini rounds.

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Re: Preferred Agar Recipes for Huatla [Re: bodhisatta]
    #25485410 -

bodhisatta said:
Vegemite is all salt so I would suggest avoiding it.
Also yeast should be autolyzed yeast extract for agar




Roger that..I'll seek out some Autolyzed:thumbup:

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Re: Preferred Agar Recipes for Huatla [Re: scab eater]
    #25485592 -

scab eater said:
I hate seeing recipes like this in percentages. is it 2% by weight, volume, also with water and powder ingredients it gets confusing. This is what i use which is probably similar.

1 teaspoon agar
2 teaspoons potato flakes
1 drop karo
1/2 cup water
1 drop food coloring

Mix together in a sauce pan on medium. Pour into jars or glad mini rounds and pc.



It's always by weight.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Re: Preferred Agar Recipes for Huatla [Re: 36fuckin5]
    #25485960 -

Oh ok!

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Re: Preferred Agar Recipes for Huatla [Re: scab eater]
    #25486182 -

Thanks for speaking about yeast. That's what fucked up my first set of plates.


--------------------
Novice mycologist, my first inoculation was 8/1/18.

Edited by Bearded Irishman (09/24/18 06:20 PM)

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