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InvisibleATWAR
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Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Simple Agar recipe please?
    #1254850 - 01/28/03 09:27 AM (18 years, 8 months ago)

I wasnt sure if this belonged here or in the Advanced forum, but I dont think it is too advanced anyway.

I obtained a few petri dishes that already had some type of material in them and now I would like to reuse them but I dont have a recipe for pouring my own plates.

My question is this:

Does anyone have a "homegrown" type recipe for my plates that doesnt require a romp all over the state tracking down ingredients? I really want to make this easy on myself, and I would rather not have to order any ingredients. Plus, living in the middle of nowhere makes it a little difficult to obtain "uncommon" ingredients.

TIA
SDYDK ATWAR


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To give is to live...



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Offlinesocratesmind
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Registered: 02/22/02
Posts: 1,193
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Re: Simple Agar recipe please? [Re: ATWAR]
    #1254898 - 01/28/03 09:40 AM (18 years, 8 months ago)

order from an online supplier. or u can make your own recipes are in the FAQ.


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Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


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OfflineShrewDigsby
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Re: Simple Agar recipe please? [Re: ATWAR]
    #1254901 - 01/28/03 09:41 AM (18 years, 8 months ago)

Go to the  [url=http://www.shroomery.org/faq/faq.php?list=categories&prog=1&lang=en&onlynewfaq=0  ]FAQ[/url].  Select Agar.  Then Select Agar Recipes....


There are many.  :grin: 


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Marijuana is a horticultural plant.  Hemp is an industrial weed.  I believe they were both provided to us by GOD to use and enjoy.


Edited by ShrewDigsby (01/28/03 09:42 AM)


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InvisiblePrisoner#1
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Re: Simple Agar recipe please? [Re: ATWAR]
    #1259857 - 01/29/03 07:26 PM (18 years, 8 months ago)

Ok...the simpleest recipe to ever come along....no one believes it works but it does

1. 3-4 packages of UNFLAVORED gelatin...store brands are fine
2. 1 1/2 cups cold water in a sauce pot
3 . 1 beef boullion cube...lower in salt

bring water to a rolling boil, ROLLING boil
crush boullion cube and stir into water
reduce heat and stir in gelatin with a fork 1 pkg at a time
remove from heat and allow it to cool for about 15 minutes, covered.
pour mixture in to petri dishes and cover.

it works, it will start to break down in about 4 days to a liquid
but thats generaly enough time to get a culture and transfer it
to another medium or another dish...I always catch flack for this
one but it does work....it just has a short life...

Prisoner#1



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Invisibledeanofmean
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Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Simple Agar recipe please? [Re: ATWAR]
    #1259919 - 01/29/03 08:02 PM (18 years, 8 months ago)

i've not seen any simpler then PDA, and i heard it works pretty good for cubies :smile: 


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OfflinesBUD
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Registered: 06/07/02
Posts: 263
Last seen: 18 years, 3 months
Re: Simple Agar recipe please? [Re: deanofmean]
    #1261361 - 01/30/03 10:31 AM (18 years, 8 months ago)

What do ya think about MEA deanofmean?  I also read how sugar was a good supplement that will increase yield....
dunno if theres any truth to that for cubies???

sBUD:tongue: 


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Invisibledeanofmean
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Registered: 12/07/02
Posts: 2,017
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Re: Simple Agar recipe please? [Re: sBUD]
    #1261421 - 01/30/03 10:44 AM (18 years, 8 months ago)

haven't tryed it .


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OfflineThebluemage
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Registered: 08/29/12
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Re: Simple Agar recipe please? [Re: Prisoner#1]
    #17043463 - 10/16/12 07:26 PM (9 years, 10 days ago)

so what exactly do I do with the culture once it starts before those 4 days? like what do i move it to???


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OfflineJcoonan
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Registered: 06/14/12
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Re: Simple Agar recipe please? [Re: Thebluemage]
    #17043629 - 10/16/12 07:54 PM (9 years, 10 days ago)

Store bought gelatin? Is pectin a viable alternative? I have some sterile petri dishes and I'm bored while my jars are colonizing, would love to give this a try. Also to ShrewDigsby, your link isn't working.


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:awecid::awecid:

Disclaimer: All posts by this user are fictious and outright forgeries of posts and pictures found elsewhere on the Internet. Do not heed any advice or suggestions as they are baseless and without merit. All posts are for entertainment and entertainment alone and should not be confused for fact or truth.


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OfflineProfessorPinHead
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Re: Simple Agar recipe please? [Re: Jcoonan] * 2
    #17043672 - 10/16/12 08:05 PM (9 years, 10 days ago)

For all the recipes you'll need some agar powder or flakes.


LMEA
(Light Malt Extract Agar)

10 grams light malt extract
10 grams agar agar

MYA (Malt Yeast Agar)

2 grams brewers yeast
10 grams malt extract
10 grams agar agar


CFA (cat food agar)

10 grams dry cat food
10 grams agar agar

OVA
(ovaltine agar)

10 grams ovaltine
10 grams agar agar

FFA (fish food agar)

10 grams fish food flakes
10 grams agar agar

RFA
(rabbit food agar)

10 grams rabbit food pellets
10 grams agar agar

PDA
(potato dextrose agar)

Take 300 grams of potatoes. Cut them and boil them in 1/2 gallon H2O for 30 minutes on a simmer. The liquid will reduce to ~ 1 litre in that amount of time.

Take the liquid and strain it out. The starches in the liquid are what you need so toss the potatoes out or make a snack with them,

Use 500 ml of the potato water as the H2O source w/

10 grams Dextrose
10 grams agar agar


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OfflineJadedEarth
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Registered: 08/08/12
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Re: Simple Agar recipe please? [Re: ATWAR] * 1
    #17043690 - 10/16/12 08:06 PM (9 years, 10 days ago)

This is my agar recipe. Use the oatmeal one it works better.
http://www.shroomery.org/forums/showflat.php/Number/16980997#16980997


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Offlineh99millerS
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Registered: 12/14/20
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Re: Simple Agar recipe please? [Re: ProfessorPinHead]
    #27088495 - 12/14/20 11:44 AM (10 months, 4 days ago)

How much water do you add to any of these recipes please.

Thanks


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Invisiblemind.at.large
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Registered: 12/13/16
Posts: 814
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Re: Simple Agar recipe please? [Re: h99miller]
    #27088538 - 12/14/20 12:20 PM (10 months, 4 days ago)

:oldthread:

Use 2% agar and 2% nutrients of g/ml

Example:
1000 ml water
20 g agar powder
20 g LME


--------------------
Mind's Easy Bag 2 Bag Grain Transfers


...the doll's trying to kill me and the toaster's been laughing at me...


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OfflineAK1000
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Re: Simple Agar recipe please? [Re: mind.at.large]
    #27088641 - 12/14/20 01:23 PM (10 months, 4 days ago)

Suuuuuuuper simple from pasty! Here ya go:

For 8-10 Pasty Plates You Will Need:
- 1 gram honey or raw sugar
- 2 grams agar
- 3 grams potato flakes (plain instant mashed potatos)
- 1/2 cup water
- optional: food dark coloring


--------------------
-AK

I'm a noob and :heart: this forum. Here's What I've Learned My First FOUR Months.

Best resources: Bods Easy AF List / Ultimate Tek Compendium


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OfflineAbabyphoenix
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Registered: 03/21/19
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Re: Simple Agar recipe please? [Re: AK1000]
    #27088650 - 12/14/20 01:26 PM (10 months, 4 days ago)

Just get real fucking lazy and get premixed since its the same damn thing.
U literally just add water mix/cook together (I like to cheesecloth it) then pc.
I buy LME and Agar agar separately and mix the two together, it makes me feel more like I'm doing something... but its just an extra step (and in the end more work on my wrists) I have both premix and seperate. :thumbup:

Shroom supply agar premix


--------------------


:sporedrop:Pho's Mushi Aventures:sporedrop: : First Agar Journey:bow2:  Pho Conquors Agar

:buddha: LAGM2021 :buddha2:


Edited by Ababyphoenix (12/14/20 01:27 PM)


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OfflineCatsLoveHouseMusic
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Re: Simple Agar recipe please? [Re: Ababyphoenix] * 1
    #27088716 - 12/14/20 01:52 PM (10 months, 4 days ago)

I did not make up this procedure so not taking credit but this is what I’ve done and always worked.

Buy the 500 Ml media bottle off amazon with an orange cap, it’s Pyrex.

Mix 8 grams telephone brand agar, 8 gram light malt extract with about 400-420ml of water. Shake it up, have the cap on loosely and put it in the microwave until it pretty much boils. Be careful for it to bubble out of the bottle, make sure cap is on loose.

Shake it up good. When you shake with cap close, it’ll build pressure FYI. Fill pressure cooker up the same height as the agar in bottle. Have cap on loose enough to relieve pressure, put tin foil on the cap. Pressure cook it.


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OfflineCatsLoveHouseMusic
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Re: Simple Agar recipe please? [Re: CatsLoveHouseMusic]
    #27088724 - 12/14/20 01:56 PM (10 months, 4 days ago)

I only get specific with brands bc when I first started I was curious about brands and stuff too. This is exactly what I have and have done a couple hundred plates


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OfflineBrian Jones
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Re: Simple Agar recipe please? [Re: CatsLoveHouseMusic]
    #27088727 - 12/14/20 01:58 PM (10 months, 4 days ago)

That is pretty much exactly what I do. Think I got it from Bodhi.


--------------------
"The Rolling Stones will break up over Brian Jones' dead body"    John Lennon

I don't want no commies in my car. No Christians either.


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 931
Loc: Gamehenge
Re: Simple Agar recipe please? [Re: Brian Jones]
    #27088862 - 12/14/20 03:25 PM (10 months, 4 days ago)

I just did my first run of plates and used the MEA recipe because I'm a homebrewer and have some leftover LME I use for yeast propagation - 10g LME, 10g telephone brand agar-agar, 500ml water and a couple drops of food coloring because I'm fancy. I didn't have a media bottle (still waiting for them to show up on my doorstep) so I rinsed out an empty rum bottle, drilled a hole in the cap and stuck a piece of tyvek under the cap. PC at 15psi for ~25 minutes.

Poured 12/8, inoculated 12/9 and I noticed my first signs of growth today (12/14) on one of my plates. Cheers, mate!

I also just realized this is a 17 year old thread but I already typed my response lol.


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