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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose

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InvisibleATWAR
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Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Simple Agar recipe please?
    #1254850 - 01/28/03 09:27 AM (14 years, 1 month ago)

I wasnt sure if this belonged here or in the Advanced forum, but I dont think it is too advanced anyway.

I obtained a few petri dishes that already had some type of material in them and now I would like to reuse them but I dont have a recipe for pouring my own plates.

My question is this:

Does anyone have a "homegrown" type recipe for my plates that doesnt require a romp all over the state tracking down ingredients? I really want to make this easy on myself, and I would rather not have to order any ingredients. Plus, living in the middle of nowhere makes it a little difficult to obtain "uncommon" ingredients.

TIA
SDYDK ATWAR


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To give is to live...



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Offlinesocratesmind
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Registered: 02/22/02
Posts: 1,193
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Re: Simple Agar recipe please? [Re: ATWAR]
    #1254898 - 01/28/03 09:40 AM (14 years, 1 month ago)

order from an online supplier. or u can make your own recipes are in the FAQ.


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Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


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OfflineShrewDigsby
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Re: Simple Agar recipe please? [Re: ATWAR]
    #1254901 - 01/28/03 09:41 AM (14 years, 1 month ago)

Go to the  [url=http://www.shroomery.org/faq/faq.php?list=categories&prog=1&lang=en&onlynewfaq=0  ]FAQ[/url].  Select Agar.  Then Select Agar Recipes....


There are many.  :grin: 


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Marijuana is a horticultural plant.  Hemp is an industrial weed.  I believe they were both provided to us by GOD to use and enjoy.


Edited by ShrewDigsby (01/28/03 09:42 AM)


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InvisiblePrisoner#1M
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Re: Simple Agar recipe please? [Re: ATWAR]
    #1259857 - 01/29/03 07:26 PM (14 years, 30 days ago)

Ok...the simpleest recipe to ever come along....no one believes it works but it does

1. 3-4 packages of UNFLAVORED gelatin...store brands are fine
2. 1 1/2 cups cold water in a sauce pot
3 . 1 beef boullion cube...lower in salt

bring water to a rolling boil, ROLLING boil
crush boullion cube and stir into water
reduce heat and stir in gelatin with a fork 1 pkg at a time
remove from heat and allow it to cool for about 15 minutes, covered.
pour mixture in to petri dishes and cover.

it works, it will start to break down in about 4 days to a liquid
but thats generaly enough time to get a culture and transfer it
to another medium or another dish...I always catch flack for this
one but it does work....it just has a short life...

Prisoner#1



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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Simple Agar recipe please? [Re: ATWAR]
    #1259919 - 01/29/03 08:02 PM (14 years, 30 days ago)

i've not seen any simpler then PDA, and i heard it works pretty good for cubies :smile: 


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OfflinesBUD
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Registered: 06/07/02
Posts: 263
Last seen: 13 years, 7 months
Re: Simple Agar recipe please? [Re: deanofmean]
    #1261361 - 01/30/03 10:31 AM (14 years, 29 days ago)

What do ya think about MEA deanofmean?  I also read how sugar was a good supplement that will increase yield....
dunno if theres any truth to that for cubies???

sBUD:tongue: 


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: Simple Agar recipe please? [Re: sBUD]
    #1261421 - 01/30/03 10:44 AM (14 years, 29 days ago)

haven't tryed it .


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OfflineThebluemage
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Registered: 08/29/12
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Re: Simple Agar recipe please? [Re: Prisoner#1]
    #17043463 - 10/16/12 07:26 PM (4 years, 4 months ago)

so what exactly do I do with the culture once it starts before those 4 days? like what do i move it to???


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OfflineJcoonan
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Registered: 06/14/12
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Re: Simple Agar recipe please? [Re: Thebluemage]
    #17043629 - 10/16/12 07:54 PM (4 years, 4 months ago)

Store bought gelatin? Is pectin a viable alternative? I have some sterile petri dishes and I'm bored while my jars are colonizing, would love to give this a try. Also to ShrewDigsby, your link isn't working.


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OfflineProfessorPinHead
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Re: Simple Agar recipe please? [Re: Jcoonan] * 1
    #17043672 - 10/16/12 08:05 PM (4 years, 4 months ago)

For all the recipes you'll need some agar powder or flakes.


LMEA
(Light Malt Extract Agar)

10 grams light malt extract
10 grams agar agar

MYA (Malt Yeast Agar)

2 grams brewers yeast
10 grams malt extract
10 grams agar agar


CFA (cat food agar)

10 grams dry cat food
10 grams agar agar

OVA
(ovaltine agar)

10 grams ovaltine
10 grams agar agar

FFA (fish food agar)

10 grams fish food flakes
10 grams agar agar

RFA
(rabbit food agar)

10 grams rabbit food pellets
10 grams agar agar

PDA
(potato dextrose agar)

Take 300 grams of potatoes. Cut them and boil them in 1/2 gallon H2O for 30 minutes on a simmer. The liquid will reduce to ~ 1 litre in that amount of time.

Take the liquid and strain it out. The starches in the liquid are what you need so toss the potatoes out or make a snack with them,

Use 500 ml of the potato water as the H2O source w/

10 grams Dextrose
10 grams agar agar


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OfflineJadedEarth
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Registered: 08/08/12
Posts: 147
Last seen: 1 year, 3 months
Re: Simple Agar recipe please? [Re: ATWAR]
    #17043690 - 10/16/12 08:06 PM (4 years, 4 months ago)

This is my agar recipe. Use the oatmeal one it works better.
http://www.shroomery.org/forums/showflat.php/Number/16980997#16980997


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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Petri Dish, Potato Dextrose

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