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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 16 years, 24 days
agar recipe
    #1433839 - 04/06/03 05:08 PM (22 years, 1 month ago)

how to make agar
There is also the following recipe.
Mix 1 boullion cube (I used beef, don't know if it makes a difference) in
100
ml water. Add 10 ml of this to 40 ml water. Add 2 pkg Knox gelatin and
heat.
Pour into petri plates. A substitute for petri plates is those little
glass
dishes you put under furniture casters.
This recipe produces an
enriched gelatin material which various
microorganisms will grow on
similarly to an enriched agar medium. The
problem with using gelatin as the
base is that many microorganisms release
proteases, which will digest the
gelatin (it's protein-based), causing it to
liquify. Agar is preferentially
used because it is a carbohydrate
derivative and thus unaffected by
proteinases. It will retain its gelled
characteristic while gelatin turns
to soup. You could buy powdered agar at a health food or Japanese

would this work

Edited by arabmobster (04/06/03 05:15 PM)

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OfflineAnnoA
Experimenter
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Folding@home Statistics
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 11 days, 13 hours
Re: agar recipe [Re: arabmobster]
    #1433899 - 04/06/03 05:38 PM (22 years, 1 month ago)

http://www.shroomery.org/forums/showflat.php?Cat=&Board=Forum2&Number=1389639


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 16 years, 24 days
Re: agar recipe [Re: Anno]
    #1434164 - 04/06/03 07:39 PM (22 years, 1 month ago)

thanks

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Invisibledeanofmean
mycophagous

Registered: 12/06/02
Posts: 2,017
Loc: PNW
Re: agar recipe [Re: arabmobster]
    #1434223 - 04/06/03 08:00 PM (22 years, 1 month ago)

yeah, i was about to say, if you want to grow bacteria, you could use chicken fat . :grin: 

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Offlinearabmobster
#1

Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 16 years, 24 days
Re: agar recipe [Re: deanofmean]
    #1434247 - 04/06/03 08:08 PM (22 years, 1 month ago)

chiken fat also works
cool

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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