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 arabmobster
#1
Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 12 years, 7 months
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 agar recipe 
#1433839 - 04/06/03 07:08 PM (18 years, 8 months ago) |
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how to make agar
There is also the following recipe.
Mix 1 boullion cube (I used beef, don't know if it makes a difference) in
100
ml water. Add 10 ml of this to 40 ml water. Add 2 pkg Knox gelatin and
heat.
Pour into petri plates. A substitute for petri plates is those little
glass
dishes you put under furniture casters.
This recipe produces an
enriched gelatin material which various
microorganisms will grow on
similarly to an enriched agar medium. The
problem with using gelatin as the
base is that many microorganisms release
proteases, which will digest the
gelatin (it's protein-based), causing it to
liquify. Agar is preferentially
used because it is a carbohydrate
derivative and thus unaffected by
proteinases. It will retain its gelled
characteristic while gelatin turns
to soup. You could buy powdered agar at a health food or Japanese
would this work
Edited by arabmobster (04/06/03 07:15 PM)
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Anno
Experimenter
Registered: 06/18/99
Posts: 24,145
Loc: my room
Last seen: 16 hours, 8 minutes
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arabmobster
#1
Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 12 years, 7 months
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Re: agar recipe [Re: Anno]
#1434164 - 04/06/03 09:39 PM (18 years, 8 months ago) |
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thanks
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 deanofmean
mycophagous
Registered: 12/07/02
Posts: 2,017
Loc: PNW
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yeah, i was about to say, if you want to grow bacteria, you could use chicken fat . 
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arabmobster
#1
Registered: 04/01/03
Posts: 317
Loc: Palestine
Last seen: 12 years, 7 months
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chiken fat also works
cool
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