blindingleaf said:

10cc per pf jar??  u crazy mofo!! 

i will say, ur brf>grain thread is the fastest leap off i have ever seen, even faster than commercial oysters.  I'm sure it would be faster than petri LI as well, but i still don't see a brf jar colonizing in 3 days, no matter what the innoculant.  ill have to give it a try tho!!  i never write something off until i try it. 
but i do like the brf slurry>bulk idea.  when i started bulk, my brf>coir was faster than grain>coir at the same ratio, and all i did was hand crumble, so breaking it up even more would increase speed id guess.
for grains tho, i still think petri LI's are easier and more efficient in the long run.

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MudaFuka said:

I thought about using a syringe but so far I have just poured the LI into each jar. All I have are 10cc syringes. I would like to get something much bigger before I star using syringes for LI inoculations. Pouring seems to work pretty well for me though.

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MudaFuka said:

I thought about using a syringe but so far I have just poured the LI into each jar. All I have are 10cc syringes. I would like to get something much bigger before I star using syringes for LI inoculations. Pouring seems to work pretty well for me though.

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Quote:

ive also seen people use it with straw logs with oysters!

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blindingleaf said:

MudaFuka said:

I thought about using a syringe but so far I have just poured the LI into each jar. All I have are 10cc syringes. I would like to get something much bigger before I star using syringes for LI inoculations. Pouring seems to work pretty well for me though.

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yea pouring is faster.  this tek will shine with grain bags too, because pouring a little more by accident won't throw off moisture as much as it will with a qt jar.  i used to put a thin layer of dry verm at the bottom of my grain bags anyway to absorb excess moisture, so thats always an easy fix to a potential problem if someone was concerned about throwing off moisture content

Quote:

ive also seen people use it with straw logs with oysters!

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that would be dope!!  i don't think petri LI would work well at all with straw logs for oysters, but slurry in a hurry would add the myc and the extra nutrients.

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MudaFuka said:

I have never made a BRF cake.

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MudaFuka said:

I plan on using this mainly to inoculate sterilised substrates for  bottle grows skipping the spawn run all together so throwing the moisture content off a bit doesn't concern me as much.

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eatyualive said:

not to raid the thread, but just to illustrate that you can use other media than agar slices. all of which use distilled water only.

you can also use this with pf cakes or sliced stem tissue. you use a larger small mouth pint jar and it fits on the end. then you can blend it up and pour it into grain jars. using liquid inoculant.

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ok  so do you just pour sterilized distilled water into the pint jar with the cake in it. or just blend the cake as is? Sorry i'm not fully understanding the process but very interested seems way to good to be true...
I have some cakes nearly colonized at the moment and would love to expand them to that extent. Oh shit just realized i can't since the blender attachment is not on that jar,DUH
so glad you said something about oster's plastic melting in pc. Do you think if I got the white model it would have been pcable?
what do you think of inoculating brf cakes with agar LI to make it colonise very fast ( since i'm scared of the tissue sample method) then use that as said above to inoculate qts of grain?
biggest question is how do you get the cake into the liquid? 

Erlik Khan said:

Wouldn't using LC kind of ruin the whole point of this TEK?  Seeing as how this tek is useful because it WOULDN'T support contams, but if you used LC then you would just be negating the benefits of this tek IMO

Oh, and good job OP.   My agar equipment is arriving on monday,  and this may be one of the first things i try when i have a good isolate/wedge.  Thanks for posting

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umm no if adding the liquid to graain jars then any contams will show up there. If you have sterile technique down then there should not be any more risk it;s just a waste of time it seems. I don't know if this is what you meant but if you were to have poor sterile technique and got minimal contam while inoculating, then the LI may have better chance (not much) because the contam may only be passed to  some of the jars whereas a lc could get contammed while innoculating with wedge and (while colonizing) spread through the entire LC making so no jars would be clean. IMO

oh yeah just gotta say i didn't think this thread could have gotten better then i read all this. You guys have some great ideas thanks again

MudaFuka said:

http://www.autoclavesale.com/media/catalog/product/cache/1/image/9df78eab33525d08d6e5fb8d27136e95/d/i/digital-autoclave-vertical-pressure-steam-sterilizer-1.png Its actually 150 liters.

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MudaFuka said:

http://www.autoclavesale.com/media/catalog/product/cache/1/image/9df78eab33525d08d6e5fb8d27136e95/d/i/digital-autoclave-vertical-pressure-steam-sterilizer-1.png Its actually 150 liters.

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