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Re: Liquid Inoculant [Re: Mr. Alien]
    #20646744 -

yeah but liquid cultures you can use over and over and over again. and they do store for years. ive done some that i stored over a year and it worked just fine. ive also contamed some to high hell. but if you use a stir plate and stir bar its much easier to get a solid looking culture. with LI your using it immediately.

the beauty of LC is that you can take a small amount of spores/agar/stem and create a huge amount of inoculant.

i think its better to start from agar to do an lc but it works just fine using pulverized stem tissue or even spores. maybe theres more risk, but theres risk with anything you do.

when i get time and find some of the info i will show you some fantastic stuff. im gonna pm you something alien.


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EAT GETS SHIT DONE


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Re: Liquid Inoculant [Re: eatyualive]
    #20646756 -

eatyualive said:
the beauty of LC is that you can take a small amount of spores/agar/stem and create a huge amount of inoculant.


But, i don't understand why you can't create a huge amount of inoculant out of LI too?.

Ok, thanks eatu!


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My Perfect wheat preparation TEK
My holy grail no pour agar tek

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Re: Liquid Inoculant [Re: Mr. Alien]
    #20646782 -

alien, you can. it just takes much more work to do so.

just making agar plates, cutting stem clone tissue or transfering some clone from a plate to another plate.

where you can make one big jar of LC and it grows itself. both methods have their advantages and disadvantages. depending on your situation one might be better for you.

to begin, i would suggest LI over Lc then try lc after you get the LI down. seeing a full bloom grown LC looks fucking cool its like magical with the light passing through the karos syrup or honey with fuzzy white growing through it.

but if you can colonize a clean agar plate and make an LI, suck some of it into a syringe. then setup an LC jar with a port and sfd and inject that LI into the lc. thats cleaner than using spores.



you know what ill just post it since its relevant. this is from another forum. when you go large you will understand that liquid is the way to go.

Some of the best growers on the net you have never heard of purely use liquid cultures.  Ever heard of high roller? If you have the skills to use lc's they can blow any g2g away with speed. Yes there might be a slight more risk using them . But the results and advantages of lcs far outweigh the risks when you do it correclty.

Ever heard  of highroller, myc. Waylitjim? these guys do cubes, exotics, woodlovers, pans all with LC's.

so here is highroller. what he does is he uses liquid cultures to inoculate corn cob spawn. then does seedling flats in a big automated grow room.
when your going large, liquid cultures can make things very easy. at the same time, if you screw up, you can screw up big.

but thats why you test everything before going big. the key here is that you can take a solid, tested, working clone from agar, 9er tek ect and inoculate a liquid culture to reproduce mass amounts of that clone in a short amount of time. yes, going agar to g2g from a plate will be faster at first. but, by the time you have that mass amount of lc ready, how many agar plates will you have to make in order to get the equivelant amount of liquid culture or inoculant? How long does it take for the plate to grow fully, then inoculate your 10 or so masters, then g2g those masters to 100 more quarts? its like the long distance runner versus the sprinter. the sprinter wins up front but at the back end, the long distance runner can go for days. with enough leftover energy to do it all over again.

your going to have to make tons of agar plates to g2g. then go from those 10 to 100 more. even if the liquid cultures takes a full week to grow out. it will colonize all those quarts in less than a week. so comparatively you did far less work using liquid cultures.

once that lc is ready, you have hundreds of quarts ready. and by the time you transfer those 10 quarts you used as masters to make 100 quarts of grain, boom all the lc's are colonized and ready for spawning. your still waiting on the g2g'ed jars to colonize. now, don't get me wrong, i like all of these methods. i work with everything myself p2g, g2g, lc, li. i love every type of cultivation out there. it is an art. you can even colonize 100 quart grain jars in 3-4 days with 1 pf cake. so by the time you take that agar plate/wedge and transfer it to 10 quarts. which may take possibly a week or two to colonize. then take those 10 and colonize 100 more, your looking at a month. whereas, i can have 100 quarts with LI or Lc colonized in a matter of less than a week and ready to spawn.

so, to each his own, but im looking at it from both positions, and i know LI blows all of them away, but LC is right up there with it. The LC might take a week more to establish but once its going its going. and you only need a small amount of clone material to make that lc. so, your only using a tiny bit of clone to make a shit ton of clone. thats the beauty of it.

highrollers example.



this was all done using liquid cultures. and thats just one small portion of the pictures from his grows.
now i hope i have demonstrated the beauty of liquid cultures and i hope that in the future others will try it and not deny it. i was lucky enough to spend years in chat with highroller. very great guy and knowledgeable.


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EAT GETS SHIT DONE


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Edited by eatyualive (10/01/14 11:03 PM)

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Re: Liquid Inoculant [Re: eatyualive]
    #20646830 -

I hate syringes since i was kid. Also Muda scared me about inyecting ports. :lol:


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My Perfect wheat preparation TEK
My holy grail no pour agar tek

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Re: Liquid Inoculant [Re: Mr. Alien]
    #20646852 -

lol. you could use rtv as an injection port. i just had a 6" needle stuck in me today for about 15 minutes. that was fun.
i can watch blood be taken with no issues. ok back on topic. well, if all you have is a blender, have at it, its super fun to do LI!:rockon:

i have to say its one of my favorite parts of the hobby.


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Re: Liquid Inoculant [Re: eatyualive]
    #20646928 -

Why you said muda that inyection ports are risky muda? Back on topic. LC could be better for storage because myc have food reserves in the liquid for long time, but then if you want to make something similar with LI you can add sugar water to the blender jar instead of steril water, so that won't be a problem with LI. But then i don't understand why somebody would want to store something for long term if we have slants, that are better suited for storage. LI is really the act of blending myc and use it right away, less risk.


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My Perfect wheat preparation TEK
My holy grail no pour agar tek

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Re: Liquid Inoculant [Re: Mr. Alien]
    #20647055 -

Damn! Highroller has a hell if a set up. Any chance you have some links to some of his grows? Maybe via pm?


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Click Here for the Motherload of Grow Info

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Re: Liquid Inoculant [Re: wildernessjunkie]
    #20647071 -



Mother fucker.


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My Perfect wheat preparation TEK
My holy grail no pour agar tek

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Re: Liquid Inoculant [Re: Mr. Alien]
    #20647093 -

These really help the situation



My favoirite toy


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Re: Liquid Inoculant [Re: blueskynoise]
    #20647502 -

yeah if you wanna talk bio efficiency hes using those thin seedling flats. ives used those before simply with the green house lids and had good success. at the same time when it came time to clean those fuckers....


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EAT GETS SHIT DONE


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Re: Liquid Inoculant [Re: blueskynoise]
    #20647512 -

Quote:
how many agar plates will you have to make in order to get the equivelant amount of liquid culture or inoculant?


It depends. If you just scrape the mycelium on the surface and transfer it with an inoculation loop the plate can regrow.


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Re: Liquid Inoculant [Re: Kizzle]
    #20647856 -

Honestly I think you're comparing 6 of 1 to a half dozen of the other.

The way I see it, once you have a clean agar culture (which is where you SHOULD be starting for a LC anyway) you'd be able to expand that to any number of plates in a week to 10 days if the culture is vigorous.  Those plates can each be LI'D into 20+ jars EASILY. 

The timeframe should be damn near identical for both methods, maybe with a slight advantage going to LI since you can look at each plate and know for damn sure it's good to go before you blend it up.

I'm not as "seasoned" as some of you guys, but this seems like a silly argument :wink:

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Re: Liquid Inoculant [Re: socraticd]
    #20647942 -

What ever works. Every one just needs to find the system that suits there needs the best. I like LI because I can use it the same day I make it and I know its clean. If some people prefer LC that's cool to. I have maid a few LCs but I have never actually used one to inoculate anything because I have no idea if they are clean or not and I don't have much time to waste on contams at the moment.


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AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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Re: Liquid Inoculant [Re: MudaFuka]
    #20648352 -

MudaFuka said:
What ever works. Every one just needs to find the system that suits there needs the best. I like LI because I can use it the same day I make it and I know its clean. If some people prefer LC that's cool to. I have maid a few LCs but I have never actually used one to inoculate anything because I have no idea if they are clean or not and I don't have much time to waste on contams at the moment.


:thumbup:

Me neither.


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My Perfect wheat preparation TEK
My holy grail no pour agar tek

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Re: Liquid Inoculant [Re: Mr. Alien]
    #20648373 -

How many CC's do you use for each inoculation?

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Re: Liquid Inoculant [Re: Flomasta100]
    #20648388 -

Muda, why you say injection ports are risky?


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My Perfect wheat preparation TEK
My holy grail no pour agar tek

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Re: Liquid Inoculant [Re: Mr. Alien]
    #20648474 -

I can't speak for Muda, but I know my own self healing RTV injection ports don't ALWAYS self heal 100%, so after I've used them a few times, I can see a visible poke-mark in them.  My concern there is that it becomes a vector for unfiltered air to be pulled in to my jars when they're cooling after a PC cycle.


Curious what Muda's take on it is, though.

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Re: Liquid Inoculant [Re: socraticd]
    #20648628 -

Something like that i imagined.... What about the Self injection ports that sponsors sells..they also have this issue?


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My Perfect wheat preparation TEK
My holy grail no pour agar tek

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Re: Liquid Inoculant [Re: Mr. Alien]
    #20648673 -

No clue.  I've never used one.

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Re: Liquid Inoculant [Re: socraticd]
    #20648694 -

socraticd said:
I can't speak for Muda, but I know my own self healing RTV injection ports don't ALWAYS self heal 100%, so after I've used them a few times, I can see a visible poke-mark in them.  My concern there is that it becomes a vector for unfiltered air to be pulled in to my jars when they're cooling after a PC cycle.


Curious what Muda's take on it is, though.


when they come out of the pressure cooker they will be hot.

The gasket will be slightly expanded, compared to when it is cool.

The tiny hole is sealed.
Once it is cooled its a different story.

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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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