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OfflineRohypnol
Vice Figurehead

Registered: 08/04/03
Posts: 267
Last seen: 2 years, 2 months
PF Cake to Liquid culture transfer?
    #2041979 - 10/25/03 03:20 PM (14 years, 52 minutes ago)

My friend attempted to innoculate a jar of karo with a peice of mycellium he chopped off a PF Cake. Before sucking up the mycellium in the needle and innoculating, he soaked the peice of mycellium in 1:1 peroxide:water.

The mycellium grew very quickly and vigerously and he decided to innoculate his jars of popcorn. The next day, a black mold formed right down each innouclating path in each jar. The culture was clearly contaminated. I have two questions.


1. How can my friend redo the experiment without the contamination? Is there any way to get dirty mycellium into karo without contamination?

2. What was the contamination? It was dark brown in colour, and very very powdery. When he dumped it in the garbage bag, a cloud of brown dust came out :frown:


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OfflineMycoCat
a.k.a. ShroomCat

Registered: 10/09/03
Posts: 1,042
Last seen: 3 years, 9 months
Re: PF Cake to Liquid culture transfer? [Re: Rohypnol]
    #2042112 - 10/25/03 04:20 PM (13 years, 11 months ago)

I don't have answers to your questions, but I'm sorry for your friend.


--------------------
No question is so difficult to answer as that to which the answer is obvious.

Meow.


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Invisiblefungusflip
visionary

Registered: 12/08/02
Posts: 284
Loc: texas
Re: PF Cake to Liquid culture transfer? [Re: MycoCat]
    #2042276 - 10/25/03 05:22 PM (13 years, 11 months ago)

i think the best way to do the karo/water tek is to inoculate the karo with spores directly. once it forms a cloudy mass of mycelium suck it up into a clean syringe and inoculate some jars or you could have a few quart jars filled with the karo/water mixture and inoculate them with the mycelium to create even more inoculant. if you did the later of the two options you could potentially have enough liquid inoculant to knock up hundreds perhaps even thousands of jars in a very short amount of time. the way your friend went about doing this with a piece of colonized cake is risky. another way this can be done is to cut a piece of tissue from the inside of a nice fruit and put it in sterile karo/water. make sure its a nice shroom cuz all of the fruits will be clones of that shroom. this also cuts down on aborts alot.


--------------------
let it be know, there is a fountain that was not made by the hands of men.


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Invisiblefeito
hillbillyhippykiller

Registered: 03/06/02
Posts: 75
Re: PF Cake to Liquid culture transfer? [Re: fungusflip]
    #2042314 - 10/25/03 05:37 PM (13 years, 11 months ago)

Use a glove box.
This works vey well. Eberbach containers work well to macerate the piece of cake. And you can PC it, so its pretty foolproof.
Be cleaner next time. :wink:


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OfflineRohypnol
Vice Figurehead

Registered: 08/04/03
Posts: 267
Last seen: 2 years, 2 months
Re: PF Cake to Liquid culture transfer? [Re: feito]
    #2042502 - 10/25/03 06:54 PM (13 years, 11 months ago)

my friend wonders if it is easier to see contamination in agar, and if he should switch to that


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InvisibleHanky
wiffle bat.
Male User Gallery
Registered: 08/30/03
Posts: 56,993
Loc: Great Southern Land.
Re: PF Cake to Liquid culture transfer? [Re: Rohypnol]
    #2042517 - 10/25/03 07:05 PM (13 years, 11 months ago)

yes it's much easier to see contam in agar...and you can just cut a clean culture from a contaminated agar plate and use it to start more agar plates or to spawn grain etc.


--------------------
Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]




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