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Offlinetombosley8
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Re: Frank's WBS Prep (perfect every time) [Re: FrankHorrigan]
    #19681330 - 03/11/14 03:29 PM (10 years, 1 month ago)

http://www.themadseason.com/90mm-Liquid-Culture-Jar-Lid-Wide-Mouth-Jars-p/3003.htmso this lid will work right? and no need for foil right? or should I make my own? Are these really reusable hundreds of times


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Edited by tombosley8 (03/11/14 03:30 PM)

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OfflinecronicrFacebook
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Re: Frank's WBS Prep (perfect every time) [Re: tombosley8]
    #19681345 - 03/11/14 03:31 PM (10 years, 1 month ago)

not for grains, not enough gas exchange there for lc's


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OfflinePussyFart
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Re: Frank's WBS Prep (perfect every time) [Re: tombosley8]
    #19681348 - 03/11/14 03:32 PM (10 years, 1 month ago)

That lid is for LCs, it would restrict too much GE for grains.

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InvisibleFrankHorrigan
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Re: Frank's WBS Prep (perfect every time) [Re: PussyFart]
    #19681408 - 03/11/14 03:43 PM (10 years, 1 month ago)

:whathesaid: :whathesaid:


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Frank's tips and tricks. Updated on 3/21/14
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Offlinetombosley8
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Re: Frank's WBS Prep (perfect every time) [Re: FrankHorrigan]
    #19681428 - 03/11/14 03:46 PM (10 years, 1 month ago)

ok cool thanks so would the bead of silicone would replace having to put the ship on? In other words is a ball of the red rtv over a hole just as good as a port i would buy and glue in? And last as long? looks like rr just slips the ship in without even gluing?


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Edited by tombosley8 (03/11/14 03:47 PM)

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InvisibleFrankHorrigan
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Re: Frank's WBS Prep (perfect every time) [Re: tombosley8]
    #19681434 - 03/11/14 03:47 PM (10 years, 1 month ago)

Yep :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Offlinetombosley8
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Re: Frank's WBS Prep (perfect every time) [Re: FrankHorrigan]
    #19681449 - 03/11/14 03:50 PM (10 years, 1 month ago)

Thanks sounds easy enough you saved me a headache figuring that all  out. three very easily obtainable supplies sounds perfect


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Offlineinvitro


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Re: Frank's WBS Prep (perfect every time) [Re: tombosley8]
    #19685810 - 03/12/14 01:17 PM (10 years, 1 month ago)

So the WBS has soaked for 22 hours or so.  I looked up the definition of simmer and came up with something like 200F-205F, or just before boiling.  I noticed that bubbles started to come up rapidly at 170F
so I dumped it 195F or so because I really don't want to ruin the grains.  When you say simmer do you mean wait for bubbles or do you mean wait for 200F, I don't think I'm splitting hairs here either because 30F is a big difference especially since you say there is a danger of ruining the WBS if you let it "boil", if boiling means going past the point of where bubbles rapidly rise then I guess I went past.

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InvisiblebodhisattaMDiscordReddit
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Re: Frank's WBS Prep (perfect every time) [Re: invitro]
    #19685849 - 03/12/14 01:25 PM (10 years, 1 month ago)

the bubbles were from the WBS not the water. Simmering you'll just barely hear the tinging ping noise and maybe a bubble or two and its 200-205 ish 212 is boiling.

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Offlineinvitro


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Re: Frank's WBS Prep (perfect every time) [Re: bodhisatta]
    #19690958 - 03/13/14 02:02 PM (10 years, 1 month ago)

I pretty much stuck to the tek except I didn't pre-rinse at all.  I have a bunch of jars with no clumping, I can shake them just fine and I waited until 12 hours after I took them out of the pc.  I never rinsed my rye either when I did rye and never ever had clumping issues.  Not only it is easier not to rinse but that rinse water contains some sugars from the grains. 

The stock pot smelled pretty rank (sour bacterial smell) after the 22 hour soak, but that's typical IME.

I just popped open a jar that I pced last night to squeeze the grains.  The millet and wheat are Al Dante but the sorghum sure isn't.  It's really pretty hard, not as hard as fresh sorghum but it's way off from Al Dante.

I just opened another jar (still hot) from another batch and the sorghum was 80-90% Aldante with a few hard ones.

I just opened a third jar from a 3rd batch and the sorgum was in-between in terms of hardness.  I could get my finger nail to split the grain but only with serious force.  Not as hard as the first batch but still not Al Dante.  Al Dante to me means slight resistance.  This was clearly more than slight resistance.  The wheat is so soft that it's not offering any resistance.

I started with hot water in a 22 quart presto PC and put the whole bag of WBS in, 20 lbs, after the water was hot, probably near 170F. 

Edited by invitro (03/13/14 02:29 PM)

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OfflineDilated
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Re: Frank's WBS Prep (perfect every time) [Re: invitro]
    #19805325 - 04/06/14 08:09 PM (10 years, 12 days ago)

Well I started my first bulk grow today and my WBS is soaking now.  Props to Cronicr for leading me to all your links Frank.  I didn't have gypsum to soak with the WBS but there is coffee soaking with it.  I was curious about tomorrow though.  I have my syringes ready from SporeWorks but I am a bit intimidated by which lids to use for my QT jars.  Could someone lead me in the right direction for getting them prepared.. It just has the standard tin lids on them that came with them.  Thanks!!

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OfflineDilated
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Re: Frank's WBS Prep (perfect every time) *DELETED* [Re: Dilated]
    #19805360 - 04/06/14 08:15 PM (10 years, 12 days ago)

Here's a link to my journal if anyone would like to follow along.  Thanks again for sharing your information Frank.

http://www.shroomery.org/forums/showflat.php/Number/19805345

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OfflineHappy Littletree
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Re: Frank's WBS Prep (perfect every time) [Re: Dilated]
    #19805407 - 04/06/14 08:27 PM (10 years, 12 days ago)

Use what's available to you. You can use the search to find info on the three main ones. Polyfil, SFD's, and tyvek (preferably from painters suits not the postal envelopes). If you're doing G2G transfers those only need the GE and the others will have an injection port made with High temp RTV gasket silicone or you can order injection ports.
sfd
http://www.shroomery.org/forums/showflat.php/Number/11487044
tyvek
http://www.shroomery.org/forums/showflat.php/Number/10628004/fpart/1/vc/1
polyfil
http://www.shroomery.org/forums/showflat.php/Number/18278624

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Offlinetombosley8
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Re: Frank's WBS Prep (perfect every time) [Re: Happy Littletree]
    #19806357 - 04/07/14 12:19 AM (10 years, 12 days ago)

hers a good tek i used: http://www.shroomery.org/forums/showflat.php/Number/14722818#14722818 make sure you use the grain jar tutorial only. Oh yea and make the holes 1/4" or smaller. 1/4 works fine for me  I used plastic lids because they will never rust and worked out great. best part is each one only costed me aboout 45 cents to make including all supplies... you can't beat that... and they're reusable hundreds of times. make sure if you use plastic to rough up the surfaces where you glue with sand paper or it will not stick. our sponsors have cheap filter discs. get the biggest ones and cut them into quarter sizes. i get 19-20 cutouts per disc at $1.75


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OfflineDilated
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Re: Frank's WBS Prep (perfect every time) [Re: tombosley8]
    #19809262 - 04/07/14 06:00 PM (10 years, 11 days ago)

So I been following Franks guide so far and I already hit a slight snag.  I followed his recipe of 10 cups of WBS hydrated and it only yielded me this


Anyways I have another batch thats been soaking for 4 hours roughly.  Is the "12-24" hour soak an exact science?  Why so long?

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InvisibleMudaFuka
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Re: Frank's WBS Prep (perfect every time) [Re: Dilated]
    #19809323 - 04/07/14 06:10 PM (10 years, 11 days ago)

the soak is what hydrates your grains so if you don't soak long enough they won't get properly hydrated. A long soak also makes  endospores to easier to kill during the PC. I would say your jars are over filled.


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AMU
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InvisiblebodhisattaMDiscordReddit
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Re: Frank's WBS Prep (perfect every time) [Re: MudaFuka]
    #19809343 - 04/07/14 06:13 PM (10 years, 11 days ago)

The jars are over filled the foil doesn't need to be rubber banded on and the foil comes off right away after you sterilize. The endospore thing is pretty much BS you actually likely have more endospores in your grain after the soak anyway but yes the soak hydrates your grain, if you start your soak with warmer water it will take less time but I don't do WBS only Rye, if you start your soak with rye near boiling you only have to soak for about 3-4 hours.

you should have put those ten cups of wbs into like ~8 jars.

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Offlinelateforthafuture
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Re: Frank's WBS Prep (perfect every time) [Re: MudaFuka]
    #19809360 - 04/07/14 06:15 PM (10 years, 11 days ago)

Also it's supposed to be 9-10 CUPS DRY = 7-10 HYDRATED QUARTS

Straight from the tek

About 9-10 cups of dry WBS equals 7-10 hydrated WBS quart jars (varies by brand)

Are you sure you measured properly, and I agree your jars look pretty full, I fill mine no more than 2/3 with hydrated grains then PC, they always seem to expand a bit after PC'ing


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"Not what could have been, or what should have been, but what will be" -Self
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OfflineDilated
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Re: Frank's WBS Prep (perfect every time) [Re: lateforthafuture]
    #19809591 - 04/07/14 06:56 PM (10 years, 11 days ago)

Yeah I measured 10 cups dry.. I mistyped and made it sound like hydrated cups.  It's alright I have another batch soaking as we speak and I'll be inoculating in the morning.  The sterlized jars should be fine till the morning right?

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InvisiblebodhisattaMDiscordReddit
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Re: Frank's WBS Prep (perfect every time) [Re: Dilated]
    #19809600 - 04/07/14 06:59 PM (10 years, 11 days ago)

they're sterilized they'll be fine for weeks. Also take foil off after PC cools down (cools down naturally) don't need to secure foil just cover the top all it's doing is stopping water dripping from the top of the PC onto your lids during the PC cycle, Some people don't even bother with the foil when using polyfil gas exchange or SFD gas exchange but I think it's a good idea to foil the lids with polyfil.

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