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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Frank's WBS Prep (perfect every time) [Re: PussyFart]
    #18613853 -

Okay good. The water was as clear as the tap water I used tho, maybe something in my water?

And that's good. I just got home from work and didn't want to have to start another batch of this stuff.
Thanks, yet again, Hacker.


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Re: Frank's WBS Prep (perfect every time) [Re: jkz]
    #18613857 -

The water will typically get "dingy looking" during the saok, but mine never gets that bad.

Mine gets to be a nasty rust color and that's it.

Just rinse them a little better next time, that's all.

I like to keep them in a big pot under the faucet and stir them, dumping off the water until it dumps off clear.

My WBS is really dirty, so usually takes 10-15 minutes of filling the pot, stirring and dumping.


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

Edited by PussyFart (07/26/13 09:24 AM)

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Re: Frank's WBS Prep (perfect every time) [Re: PussyFart]
    #18614041 -

Yeah, I agree, mine takes a lot of rinsing too.

If the water ran clear though, the water ran clear :thumbup:

I agree, you can use them jkz.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: Frank's WBS Prep (perfect every time) [Re: FrankHorrigan]
    #18615647 -

FrankHorrigan said:
mthawkins said:
During inoculation, do I need to have a glove box or is it ok without?

Also, can I use folgers instant coffee? how many teaspoons?


You need to do all your work with sterile media, grains included, inside a still air box.

And I would not use instant coffee nor do I have any recommendation on how much to use.



Alright. I was under the impression that glove box and sterilization wasn't needed when doing this with the pasteurized bulk method too (other than the needle.)

Any links on a suggested way to inoculate? and how to make a glove box?


--------------------
“Never laugh at live dragons.”
- J.R.R Tolkien

Edited by mthawkins (07/26/13 03:42 PM)

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Re: Frank's WBS Prep (perfect every time) [Re: mthawkins]
    #18615657 -

You can spawn to pasteurized bulk substrate in open air.

You need to prepare all of your grains and cultures under aseptic conditions like in a SAB regardless though.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: Frank's WBS Prep (perfect every time) [Re: FrankHorrigan]
    #18615702 -

Is there a way where I can avoid needed a glove box? Possibly clothless after a shower, sterilize the air and working area, gloves?
I'm using this tek for jar lids if it matters: http://www.shroomery.org/forums/showflat.php/Number/10628004#10628004


--------------------
“Never laugh at live dragons.”
- J.R.R Tolkien

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Re: Frank's WBS Prep (perfect every time) [Re: mthawkins]
    #18615744 -

You can prepare and sterilize your jars without one, but inoculating them in open air is totally different.

You can expect some success and a lot of failure without one.

This is one area you should not cut corners, inoculating grains and such.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: Frank's WBS Prep (perfect every time) [Re: FrankHorrigan]
    #18615892 -

Wouldn't a flame sterilized needed with self healing lids be as safe as a dry verm layer on pf cakes? Or are you specifically referring to G2G transfers and such.


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Re: Frank's WBS Prep (perfect every time) [Re: jkz]
    #18616555 -

It would be about as safe risky to inoculate cakes in open air as it is grains.

Sorry man, you won't find a way around this answer from me :shrug:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: Frank's WBS Prep (perfect every time) [Re: FrankHorrigan]
    #18617775 -

After inoculation where should I store the jars? Would a closet be ok? For how long, usually?


--------------------
“Never laugh at live dragons.”
- J.R.R Tolkien

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Re: Frank's WBS Prep (perfect every time) [Re: mthawkins]
    #18618522 -

mthawkins said:
After inoculation where should I store the jars? Would a closet be ok? For how long, usually?


On a shelf in room temperature getting ambient/indirect light until they are fully colonized.


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: Frank's WBS Prep (perfect every time) [Re: PussyFart]
    #18619094 -

Just wanted to share something I tried and see what you thought about it Frank.

Ive been following this tek for my last 3 WBS preps but the last one I skipped the simmering part. After my 24 soak I drained, rinsed one more time, then let it drain for an hour shaking occasionally. After PC 90 minutes I filed my jars, g2g, and they have been colonizing just as well as the ones I simmered...maybe even a little faster. Do you think the simmering step is really necessary?

Edited by bEelzeBosS (07/27/13 10:06 AM)

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Re: Frank's WBS Prep (perfect every time) [Re: bEelzeBosS] * 1
    #18619102 -

I do think it is necessary if you want proper moisture content consistently.

Moisture content that is off will still work as long as it isn't too bad.

But there is only so much water that can fall off or be shaken off the exterior of the grains due to molecular cohesion.

Steaming them dry is the most effective and quick way to achieve proper moisture content IMO.:thumbup:

PS. Never rinse the grains after the soak, you are washing off all of the beneficial gypsum that they are coated with :shake:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: Frank's WBS Prep (perfect every time) [Re: FrankHorrigan]
    #18645402 -

I read somewhere that when inoculating 1 quart WBS 2.5cc would be ok, is that right? Also, is there a certain amount of time you can leave them alone after the jar is fully colonized without it getting ruined?

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Re: Frank's WBS Prep (perfect every time) [Re: jackalope9517]
    #18669460 -

do you soak in hot water...or cold water?

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Re: Frank's WBS Prep (perfect every time) [Re: eelrd72]
    #18669541 -

Cold tap water is fine.

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Re: Frank's WBS Prep (perfect every time) [Re: winstonbox]
    #18672271 -

Can I use polyfill and a rtv port for jar lids ? Do I need to tape the polyfill before pc, or will it be ok?


--------------------
“Never laugh at live dragons.”
- J.R.R Tolkien

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Re: Frank's WBS Prep (perfect every time) [Re: mthawkins]
    #18672376 -

jackalope9517 said:
I read somewhere that when inoculating 1 quart WBS 2.5cc would be ok, is that right? Also, is there a certain amount of time you can leave them alone after the jar is fully colonized without it getting ruined?


1 quart should only need .5cc of spore solution or 1-2cc of liquid culture. And you can leave them for a few days after full colonization but I would use them ASAP. No need to consolidate them or anything.

eelrd72 said:
do you soak in hot water...or cold water?


Hot water.

winstonbox said:
Cold tap water is fine.


I use hot water to start every soak because I am trying to get a nice bacterial bloom in there before sterilizing. Cold water does not encourage this like hot or room-temp water does.

mthawkins said:
Can I use polyfill and a rtv port for jar lids ? Do I need to tape the polyfill before pc, or will it be ok?


Stuff the poly as tight as you can possibly stuff it and it will work fine as a filter. Just put foil over your lids before PC'ing.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: Frank's WBS Prep (perfect every time) [Re: FrankHorrigan]
    #18672490 -

Thanks frank.
I just made a booboo. Washed my seeds after simmer.. Realized I shouldn't do that and now I'm just letting it sit in the strainer


--------------------
“Never laugh at live dragons.”
- J.R.R Tolkien

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Re: Frank's WBS Prep (perfect every time) [Re: mthawkins]
    #18672573 -

I accidentally got water all over a batch once, while it was in the strainer.

I cheated, I filled a pot back up with water, put the grains back in, and brought to a simmer then dumped and let it steam. It worked :shrug:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Shop: North Spore Bulk Substrate   Original Sensible Seeds Autoflowering Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Substrate Bags   MagicBag.co Certified Organic All-In-One Grow Bags   Sporeworks.EU Spores for European Microscopy

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