Drum Roll Please! Open air inoculation is the biggest waste of time. I put this together because its so damn funny.
"1 cubic foot of normal household air contains on average: 100,000 invisible particulates. So, every square inch of that air carries: One hell of a load of possible contaminate spores. Agar
Best success rate for open air inoculation. Is to continuously keep the needle covered. With an alc saturated gauze pad, foam, paper towel, or cotton balls. Otherwise, you will not BE LUCKY, FOR LONG." Agar
Anyone who recommends open air inoculations can be compared to a drunk who recommends drinking and driving. RR
Agar is a very experienced grower. His method you linked keeps the needle covered at all times in an alcohol soaked cloth. That's actually not 'open air'. Leave the needle in open air and see what happens. A glovebox is very easy to make. RR
dgls_jms
"id say a glovebox is a waste considering i never use one and rarely if ever get a contamination
I agree, I do all my work on a cookie sheet, door open to a hot oven. Is a little warm but hasent fail me yet."
"so I had a WBS jar that started showing contams a couple days after the myc started growing. I put it on my back porch and forgot about it. Now I'm not going to use but but the battle for life inside that jar has been amazing to watch. The myc pushed the mold to the very top, just a very few grains, and took over the whole jar. Fun to watch!"
"I ordered up 4 syringes from ralph. I also ordered a "sterile liquid culture" in a qt mason jar off of ebay. They ebay dude was taking forever to ship so I went out and bought some baby bottles, did my best to sterilize in the mic, knocked up after they cooled down. 30 hours later, mycelia is covering the bottom, so I swirled to distribute it throughout the culture. After I swirled, they became cloudy and remained that way, never saw the mycelia back at the bottom. This is when I realized I should invest in a pc, so I ordered one up, should arrive today. So while I'm waiting for my pc, my "sterile lc" arrived. It was clear and apeared to be contan free. knocked it up with a cc from a fresh spore syringe. 24 hours later, bottoms covered in mycelia. So last night, I swirled to disrtibute it. This morning the jar is all cloudy, and there is not as much mycelia on the bottom as last night. I am starting to think it is either ralphs syringes, or the "sterile aiport syringe" that this guy sent me. I figured when he said sterile it would still be in the autoclave bag, but it was a syringe stuffed with polyfill, no plunger, with the needle already attached, whole think rapped in foil, in a ziplock bag. I used ipa and flamed the needles. Also, It got really could here over the past week, went from 80's to around 70, dropping into the 50's at night. MY thermostat reads 70 inside, but I think it might get lower during the night, as the heater is not turned on yet. I know this would slow myclia growth, but would it speed up bacterial growth? I was thinking about setting the jar on a heating pad thing, but I don't want it to get too hot. Any help would be greatly appreciated. Thanks"
sHrOoMaCiDe
"On one of my recent attempts, i used 6 BRF/Verm jars and cubensis spore syringes. I inoculated in open air(dumb idea) all six jars about 3 weeks ago. The result: 4jars infected with green/black molds, and the other 2 surviving jars did not colonize or change at all. No growth. What happened? I inoculated 1-2 maybe even 3cc's in each jar. Still after 3 weeks, nothings happened. Any insights for me? Seems like failure. Really disappointing ya know?"
I got extremely lucky with innoculating my LC and fungi rye bag in open air. They are both progressing without contamination. However, the bag is progressing at a glacial speed. The LC is almost done colonizing. When its done should I suck up some of that mycelium and inject it into my fungi bag to speed it up?
I've never had success in growing mushrooms yet, but my 3rd attempt was the closest. I inoculated two quart sized pre-sterilized rye grain jars back in july. The mycelium grew in one of them but not the other. However, it grew about halfway up the jar and then stopped. It went dormant or something. so my question is, can i re-inoculate the jar that didn't grow any mycelium? its really old, but theres no contamination.
VampireSlayer Why i'm done with OPEN AIR INOCULATIONS
"Just wanted to say...i did open air innoc for a year with pretty good success. actually only till the last couple months have I been getting contams...I do not fuck with open air period!! it is just a 50/50 in my oppinion. I put the blame on a vendor here about a contammed syringe but NOW i'm not sure if it was the the vendor to blame or if it was me...the point is if you use any other method (flow air or still air) then you can easssily put the blame on the culture or syringe (talking cubie syringer growers here not graintograin and stuff) but grain to grain I WOULD NEVER do in open air but NOW i'll never even do open air period...it's too risky period"
NinjaGaiden
"I've had 100% success with using the oven tek. I do everything: Make syringes, inoculate jars, G2G transfers using the oven.
I'm not at all trying to debate if the tek itself is bogus or not, but it does work for some." NinjaGaiden
"more like 100% LUCK
and I will debate it...
the Oven tek is crap!~" Roadkill
jeetered
"i always open air inoculate. I like "glacial" that's great, i've found that with spore to grain before too. LC to grain is much much faster. I don't know anything about adding LC to it to speed it up though."
"i do pretty much everything open air except agar work."
Re: What in the world! Contamination Forum jeetered 03/25/07 06:58 PM Re: Contamination??? Contamination Forum jeetered 05/17/07 08:43 PM Re: Contamination??? Contamination Forum jeetered 05/18/07 07:51 PM Re: Bacillus contam in rye Contamination Forum jeetered 07/01/07 09:47 AM black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 08:08 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 08:34 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 10:44 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 10:51 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/08/07 05:37 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/09/07 05:31 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/10/07 07:54 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum
figgusfiddus
"Yeah, open air inoculation isn't really that risky. When I get screwed up, it's usually the substrate conditions that do it."
Re: Contamination? Contamination Forum figgusfiddus 05/10/07 01:15 PM Re: Contamination? Contamination Forum figgusfiddus 05/14/07 10:39 AM Re: Daaaaaaaamn! Green Mold... Contamination Forum figgusfiddus 06/13/07 06:53 PM Re: weird growth on cake (pic) Contamination Forum figgusfiddus 06/16/07 05:05 PM Re: weird growth on cake (pic) Contamination Forum figgusfiddus 06/16/07 08:28 PM Re: What kind of infection is this? [pics] Contamination Forum figgusfiddus 06/16/07 08:50 PM Re: What kind of infection is this? [pics] Contamination Forum figgusfiddus 06/16/07 08:57 PM Re: Daaaaaaaamn! Green Mold... Contamination Forum figgusfiddus 06/17/07 02:19 PM Re: weird growth on cake (pic)update Contamination Forum figgusfiddus 06/17/07 06:13 PM Re: wet looking caps Contamination Forum figgusfiddus 06/17/07 06:15 PM Re: weird growth on cake (pic) Contamination Forum figgusfiddus 06/18/07 02:49 PM Re: ...is this Trich? Contamination Forum figgusfiddus 07/06/07 01:27 PM Re: ...is this Trich? Contamination Forum figgusfiddus 07/06/07 01:28 PM Low humidity promoting molds (esp. trichoderma)? Contamination Forum figgusfiddus 07/28/07 04:13 PM Re: Low humidity promoting molds (esp. trichoderma)? Contamination Forum figgusfiddus 07/30/07 10:27 PM Re: If i have a piece of bread with green mold on it... Contamination Forum figgusfiddus 01/30/08 03:46 PM
myndreach
"do most people here actually use a glovebox or something? SWIM has never had contams as a result of air...the one they got was because of a bad syringe.
lysol for surfaces, oust for the air, dust mask and rubber gloves."
Re: Worst Contam Pic Contamination Forum myndreach 05/27/05 08:55 AM Re: tiny black spec on mycelium Contamination Forum myndreach 06/01/05 04:26 PM First grow...AFOAF is freaking out...come this far....contam? Contamination Forum myndreach 06/10/05 09:27 PM Re: First grow...AFOAF is freaking out...come this far....contam? Contamination Forum myndreach 06/10/05 09:50 PM Re: black stuff in syringe Contamination Forum myndreach 06/11/05 10:48 PM Re: Possible contam on mushrooms? Contamination Forum myndreach 06/12/05 08:22 PM Re: brown mycellium? Contamination Forum myndreach 06/12/05 08:23 PM Re: spots on white mycelium Contamination Forum myndreach 06/13/05 09:00 AM Contam or Myc Piss? Contamination Forum myndreach 06/13/05 11:06 AM Re: Contam or Myc Piss? Contamination Forum myndreach 06/14/05 07:27 AM Contams in jar? Brown/Orange/Wet spot mold? Need help quick please. Contamination Forum myndreach 07/02/05 09:51 AM Re: Question about appearance of some recently acquired dried shrooms Contamination Forum myndreach 07/07/05 04:59 PM wet spot mold, brown mold, or just too moist? Contamination Forum myndreach 07/11/05 09:00 AM Re: wet spot mold, brown mold, or just too moist? Contamination Forum myndreach 07/11/05 09:44 AM Is this Jar okay? Please help. Contamination Forum myndreach 07/13/05 09:55 AM Re: Is this Jar okay? Please help. Contamination Forum myndreach 07/13/05 12:46 PM Re: Is this Jar okay? Please help. Contamination Forum myndreach 07/13/05 12:59 PM Re: discoloration Contamination Forum myndreach 07/14/05 09:25 AM Re: Are these mushrooms ruined? Contamination Forum myndreach 07/18/05 01:20 PM Is this jar okay? Please look and let me know. Contamination Forum myndreach 07/18/05 02:31 PM Re: Is this jar okay? Please look and let me know. Contamination Forum myndreach 07/18/05 05:17 PM Re: Is this jar okay? Please look and let me know. Contamination Forum myndreach 07/18/05 05:25 PM What is this? Are these salvagable? Contamination Forum myndreach 04/22/07 07:30 AM Re: what is this black stuff in my PF jar? Contamination Forum myndreach 04/22/07 07:42 AM Re: Weird Contamination Forum myndreach 04/22/07 07:44 AM Re: What is this? Are these salvagable? Contamination Forum myndreach 04/24/07 10:31 AM Re: hello all....first post here....got a question Contamination Forum myndreach 04/24/07 10:34 AM Myc piss, right? (pic) Contamination Forum myndreach 04/30/07 09:54 AM Re: Myc piss, right? (pic) Contamination Forum myndreach 04/30/07 10:42 AM Re: Cobweb mold? Contamination Forum myndreach 05/26/07 10:44 PM Pan cyan tray...what's going on? Contamination Forum myndreach 05/29/07 02:35 PM Re: Pan cyan tray...what's going on? Contamination Forum myndreach 05/29/07 08:01 PM
ButterWeasels
"ive inoculated many jars and ive only had 1 contam in las vegas but i didnt make the jar, it was from pjars, my very first grow. just flame sterilize often and youll have no problems in open air, in a clean house."
Re: Should I just case these cakes? Contamination Forum ButterWeasels 12/15/06 03:28 PM Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 07:42 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 07:51 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 08:46 AM Cobweb? Monotub Problems Contamination Forum ButterWeasels 10/18/07 09:25 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 10:46 AM Re: Cobweb? Monotub Problems Contamination Forum ButterWeasels 10/18/07 11:15 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 01:13 PM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 03:20 PM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/19/07 06:47 AM
I could go on to find a million of these!
|