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Anonymous

Re: a time saver for growing outside?
    #85863 - 05/25/00 01:20 PM (23 years, 6 months ago)

oh yeah i was also planing on putting a tuperware container over this like in the ryche hawk outdoor tek to seal in humidity because i live in arizona.


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Anonymous

Re: a time saver for growing outside?
    #85865 - 05/25/00 04:11 PM (23 years, 6 months ago)

Usually, compost is a little too resistant to spore germination for a direct inoculation to work. The benefit to inoculating with grain spawn is that the already healthy and aggressive mycelium colonizes the compost before other spore contaminants have a chance to take hold. If you want to grow outdoors, I don't think there is any way around first colonizing a rich grain substrate first.

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When the going gets weird, the weird get wired.



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OfflineLizard King
King Lizard

Registered: 10/03/99
Posts: 1,998
Loc: GA
Last seen: 17 years, 3 months
Re: a time saver for growing outside?
    #85866 - 05/25/00 05:56 PM (23 years, 6 months ago)

Then you need to read this thread of mine, SpaceFarmer. Its the only thing I've come up with that doesn't use grain as substrate. Basically you transfer cultures directly into dung. I used brown rice flour paste(just water and BRF) to start my cultures. Although I'm sure agar would work also. My patch has been kickin' ass for a few weeks now. I think I've found my preferred outdoor method. It works awesome, and the major advantage is there is no grain in your patch for bugs, slugs, and snails to feed on.

Heres my original thread.

Well it won't be long and I'll have a digital camera, just ordered it. But for now I'm stuck with a shitty disposable. It works for now.

These are pics of my latest experiment fruiting from wild/native spores. My goal behind this experiment was to find a way to fruit from wild(contaminated)prints, and end up with beutiful wild cubensis without using any grain as substrate.

This is what I did. I took some old dried out cow dung from a nearby field I frequent. The dung was shreaded and place in a bucket with enough water to cover the dung entirely. This was left overnight to soak up all the water possible. Now the dung is ready for use.

Now this is the experiment part. I used funky balloons Brown rice flour paste idea. I made the BRF paste and filled six 1/2 pint jars 1/4" full with this paste. Each jar had one hole punched in the center of the lid and was covered with a strip of tape. I then pressure cooked it at 15psi for 20 minutes. I let the jars cool for about 2 hours. Then I took a print of a wild Ps. cubensis I found here in Ga. last year, and scraped a chunk of spore in each jar while quickly peeling back the tape. Sterility is not important. Your print is by no means sterile if its from the field.

It should take 3-4 days to see mycelium growth(spore germination) Let the mycelium cover the entire surface. Discard the jar if any olor besides white is present(only one out of 6 contaminated on me) Get your dung ready for use. You'll need a filter patch spawn bag(ziplocks will do fine) Your going to fill the spawn bag with dung a quarter the way full. Then get your jars out and thump out the BRF paste(cake)out of the jars. I used a razor blade to slice the mycelium from the paste cake. Take as little paste with the mycelium as possible. Each jar should produce 4 even wedges of mycelium. Now place these four wedges sporadically throughout the dung. Then fill the dung another quarter the way full, and add your mycelium wedges. Do this until the bag is full off dung and mycelium wedges.

Now incubate in warm place until fully colonized. It took my bag about a week and a half to fully colonize. Then dig a hole, lay down some damp soil, plop out your dung block, and cover with 1/4" of moist soil. Cover the entire patch with pine needles and leaves to retain moisture. Misy patch when top casing looks dry. Wait for your fruit. Took mine about a week to fruit.

This tek is as native as you can get. The only grain that was used was to start cultures. Then the cultures were sliced from the paste cake and spawned to dung from the field. It worked well for me, so I thought I'd share. If your read this long message you've done well. Sorry for rambling on, I tend to do that.




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Anonymous

Re: a time saver for growing outside?
    #85867 - 05/27/00 02:59 AM (23 years, 6 months ago)

Sounds like an interesting method, but, if you are just replacing grain spawn with BRF spawn, wouldn't the bugs n' slugs still have the BRF to feed off of? If pests are a problem from too much grain, one could just use less grain spawn to compost. Only so much is really necessary anyway. This is not to say that your approach does not have validity, I just prefer a more sterile and time-tested approach.

------------------
When the going gets weird, the weird get wired.



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OfflineLizard King
King Lizard

Registered: 10/03/99
Posts: 1,998
Loc: GA
Last seen: 17 years, 3 months
Re: a time saver for growing outside?
    #85868 - 05/26/00 10:26 PM (23 years, 6 months ago)

There is abesolutely NO grain spawn in my patch. I started cultures on BRF paste, then when the cultures were ready to transfer, I sliced then from the BRF paste. Meaning all I took was mycelium, and no BRF. I used a razor to cut the mycelium from the paste cake, then transferred the culture wedges directly to dung.

If your looking for sterility outdoors, your looking in the wrong place. Thats why I came up with this idea, so that sterility is NOT at all a factor. Since there is no grain for contaminants to feed on, contamination will be obsolete. I used a wild culture and cow dung straight from the field, almost exactly the way nature does it. My results were identical to native Cubensis.


The Lizard,

[This message has been edited by Lizard King (edited May 27, 2000).]



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Anonymous

Re: a time saver for growing outside?
    #85869 - 05/28/00 04:54 PM (23 years, 6 months ago)

Aaah, I see now. The BRF paste was used to supply nutrients for the mycelium, and then you removed the mycelium from the BRF paste. That is a damn slick idea. I might have to try that if I can keep the compost from drying out completely in this climate.

Thanks, Lizard. :biggrin:

------------------
When the going gets weird, the weird get wired.



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