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InvisibleDirtmaster
addict
Registered: 11/21/00
Posts: 194
Re: Lets start fresh...DMT substrate [Re: jonnyshaggs420]
    #317162 - 05/15/01 01:51 AM (21 years, 4 months ago)

you have no idea what you're talking about. in this context, two methyl groups is an enormous difference, so is one oxygen atom.



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Offlinejonnyshaggs420
Pooh-Bah

Registered: 08/09/00
Posts: 1,965
Loc: Mid-West
Last seen: 17 years, 1 day
Re: Lets start fresh...DMT substrate [Re: Dirtmaster]
    #317168 - 05/15/01 01:59 AM (21 years, 4 months ago)

If we were talking about complete diffusion into a normal cell than it would be completely different, but seeing as these cells are specialized for nutrient uptake they are not that selective, somewhat like the roots of plants.  If you have alot of metals in your soil your plants will take them into there system and store them.  If the mycelium will take it up then it will use it.

I can see a world where this is no poverty and no war, I can also see us attacking that world because they would never expect it. :wink:


--------------------
Vote Jonnyshaggs in the next election for GOD...Its the responsible choice


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OfflineBleuboxo
enthusiast
Registered: 04/06/01
Posts: 196
Loc: Geographic Location (Stat...
Last seen: 21 years, 2 months
Re: Lets start fresh...DMT substrate [Re: jonnyshaggs420]
    #317214 - 05/15/01 02:40 AM (21 years, 4 months ago)

well hey, enough speculating, we'll specualte after someone tries it and posts results. not a flame. its just stuff we dont even need to get in to. if it works hey good job for us for discovering it. let it be known that i have just ordered 50g of mimosa bark and it should get here within a week. after that about 10-12 days til jars are fully colonized and ready to be cased. after that im going to add all of this bark to the casing and see if that works. in a month expect results.

" Insanity is just a thread of reality...the make-as-you-go part of living, the bare second reflex of dying _Stavros Christou... by_"


--------------------
" Insanity is just a thread of reality...the make-as-you-go part of living, the bare second reflex of dying _Stavros Christou... by_"


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InvisibleDirtmaster
addict
Registered: 11/21/00
Posts: 194
Re: Lets start fresh...DMT substrate [Re: Bleuboxo]
    #317254 - 05/15/01 03:04 AM (21 years, 4 months ago)

bleuboxo, i think we are in agreement. please do experiment.

the only problem is that you'll never know if it actually does raise potency since you don't have a lab, and thus neither can grow batches under controlled conditions nor gauge potency with acceptable accuracy.

as to johnny's statement that it is certain the mycelium will make more psilocybine given that it does absorb the dmt, that's incorrect. it might just as well inhibit the production of psilocybine.



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OfflineDarK_SavioR
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Registered: 05/06/01
Posts: 454
Loc: Down the Street
Last seen: 20 years, 4 months
Re: Lets start fresh...DMT substrate [Re: Dirtmaster]
    #317270 - 05/15/01 03:17 AM (21 years, 4 months ago)

Either way there's three possible turnouts... 1) it works, more potent shrooms, 2) It does nothing at all, 3) its worse than using normal casing.
Only one way to find out. As for the potentcy of the shrooms. We're not gonna be getting down to the specific percentage (at least not right now), I'm pretty sure you could tell if the shrooms are more potent than normal... if so, thats when we'll get down to the specific percentages. As for now, all we can do is guess and check. Everyone is making good points as to what could happen, but in the end everyone is just making guesses based on what they know. Saying its gonna work is just as ignorant as saying it isn't going to work, all anyone can say right now is that it might work.

Blueboxo... You should innoculate other cakes with the same spores and case them normally just to compare results in the end.

Edited by DarK_SavioR on 05/14/01 08:20 PM.



--------------------
Vitamin C chase, kill the taste. You can tell its nasty by the look on my face.
Ralphster44 & The FSR!
All thats stated above is for humor and a lie!!


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Anonymous

Re: Lets start fresh...DMT substrate [Re: Dirtmaster]
    #317278 - 05/15/01 03:23 AM (21 years, 4 months ago)

And how are you better equiped then everyone else at this BB? I don't find chemistry boring!! Explain to us how mycelium won't take up any of the substances recommended on this or any other related post. Why won't the mycelium absorb DMT, but will absorb Pure tryptamine? Teach us!!! Everyone here is very interested. I passed college biochem.



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InvisibleDirtmaster
addict
Registered: 11/21/00
Posts: 194
Re: Lets start fresh...DMT substrate [Re: ]
    #317365 - 05/15/01 04:37 AM (21 years, 4 months ago)

sorry, i'm tired of repeating myself, i have explained several times already. do what you will and good luck.



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OfflineBleuboxo
enthusiast
Registered: 04/06/01
Posts: 196
Loc: Geographic Location (Stat...
Last seen: 21 years, 2 months
Re: Lets start fresh...DMT substrate [Re: Dirtmaster]
    #317581 - 05/15/01 08:57 AM (21 years, 4 months ago)

well actually my whole room is a lab. i live with my girlfriend in a one bedroom ghetto apt. in DC...so i just turned the bedroom into a sterile environment. the walls are covered with cardboard, the floor is vinyl tile ( one big tile ). its very easy to clean and many containers in the room. i was planning to do one casing with mimosa, and the others without. trust me, if it raises potency i will know it. the strain is B+ and cambodian. these two strains will be cased with mimosa and the other casings wont. ive ate them enough to say i know the regular dosage. its about 2g for a light trip. if i eat 1g off the mimosa casings, and they give me a buzz, i know it has worked. i just sold all my lab equip. so i cant do any testing either, but i never was that advanced in the 1st place.

" Insanity is just a thread of reality...the make-as-you-go part of living, the bare second reflex of dying _Stavros Christou... by_"


--------------------
" Insanity is just a thread of reality...the make-as-you-go part of living, the bare second reflex of dying _Stavros Christou... by_"


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OfflineExplorer
Stranger
Registered: 05/04/01
Posts: 24
Last seen: 17 years, 11 months
Re: Lets start fresh...DMT substrate [Re: Dirtmaster]
    #317674 - 05/15/01 10:34 AM (21 years, 4 months ago)

Hey, Dirtmaster, your brain does it all the time. Your brain manufactures DMT br methylating tryptamine twice. All the mycelium needs to do is remove one oxygen atom. Dr. Richard Strassman, in DMT - The Spirit Molecule, describes Psilocin as "orally active DMT". Certain Psilocybe strains even contain tiny amounts of DMT, which would suggest that the mycelium is using the accepted conversion path from tryptophan to psilocin, via DMT. Where else is it getting the psilocin from? It will take Tryptamine, Tryptophan, or perhaps any chemical on that path of precursors, as raw materiial for making psilocin/psilocybin. This isn't exactly speculation if we're following someone elses work, is it? That's what I'm doing. I read that DMT added to substrate made shrooms up to 4 times more potent. Why should that work, I thought? Then I began studying. It does make sense.

And, Bleuboxo, I think the DMT was added earlier, for the mycelium to feed on, prior to innoculation. The mycelium should be exposed from the word go, that way the mycelium will already be super-potent before it's left the jar. Could be wrong, but that's how it was reported. Just casing with mimosa bark won't give you much of a hike. In the experiment I'm following, 100mg of pure, crystaline N.N.DMT was added to a 50/50 rice flour vermiculite mix in a half=pint jar. This was then innoculated, colonized and fruited as normal to produce these super shrooms. If Mimosa bark is to be used without extraction, I'd say it would have to be used with something like vermiculite as the initial substrate to innoculate.

Edited by Explorer on 05/15/01 03:45 AM.



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OfflineExplorer
Stranger
Registered: 05/04/01
Posts: 24
Last seen: 17 years, 11 months
Re: Lets start fresh...DMT substrate [Re: Explorer]
    #317681 - 05/15/01 10:49 AM (21 years, 4 months ago)

For information on the theory behind extraction of DMT from organic sources, check this manual out.

http://www.tryptamine.net/dmt/

It is free to download and distribute, just don't take credit for it and don't make any money out of it.



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OfflineDarK_SavioR
addict
Registered: 05/06/01
Posts: 454
Loc: Down the Street
Last seen: 20 years, 4 months
Re: Lets start fresh...DMT substrate [Re: Explorer]
    #317740 - 05/15/01 03:41 PM (21 years, 4 months ago)

wow, thats a nice site. Thanks for posting that up, I'm sure it can/will come in handy ; )

"Now chew em up and slam the orange juice. Vitamin C chase, kill the taste. You can tell its nasty by the look on my face."



--------------------
Vitamin C chase, kill the taste. You can tell its nasty by the look on my face.
Ralphster44 & The FSR!
All thats stated above is for humor and a lie!!


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Invisiblesynaptic
newbie
Registered: 05/23/01
Posts: 36
Re: Lets start fresh...DMT substrate [Re: DarK_SavioR]
    #330329 - 05/31/01 10:35 AM (21 years, 3 months ago)

maya-ethnobotanicals.com -- fast service, good customer support
has pre-ground mimosa hostilis root bark "incense"..
my friend snagged 150gm for $64US with shipping.. received in a week from around the world.
product looks nice.. have yet to extract it



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Invisiblesynaptic
newbie
Registered: 05/23/01
Posts: 36
Re: Lets start fresh...DMT substrate [Re: Humidity]
    #330331 - 05/31/01 10:39 AM (21 years, 3 months ago)

> L-tryptophan --> tryptamine
> tryptamine --> N-methyltryptamine
> N-methyltryptamine --> N,N-dimethyltryptamine
> N,N-dimethyltryptamine (DMT) --> psilocin & psilocybin

Instead of seeking to boost psilocin/psilocybin levels, I think another important quest would be to disable the reaction that converts DMT to psilocybin/in. DMT producing mushrooms would be a far greater renewable resource than harvesting root bark from Mimosa trees that take years and years to grow.

Sure, DMT isn't active orally without MAO inhibitors. Someone said that Psilocybin/in is a more potent tryptamine than DMT. I don't think that's true at all. I think DMT is just more readily destroyed by the body than psilocybin/in because it's part of the normal chemistry of the body. DMT seems like far more potent, imho. There are good points to a 30-60 minute duration too.



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OfflineOpi
newbie

Registered: 11/13/00
Posts: 22
Last seen: 20 years, 5 months
Re: Lets start fresh...DMT substrate [Re: synaptic]
    #330368 - 05/31/01 12:13 PM (21 years, 3 months ago)

Which is more potent depends on if you judge potency in milligrams, or by a typical dose.

OPI



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Offlineuneasyone
tokin theMacGyver bong

Registered: 06/12/03
Posts: 299
Loc: SE united states
Last seen: 8 years, 8 months
Re: Lets start fresh...DMT substrate [Re: Humidity]
    #1649347 - 06/21/03 03:48 AM (19 years, 3 months ago)

why bother with extraction why not just order dmt from jmar chemical co. they'll sell it to anyone and it would be alot more convenient
http://www.jmarchemical.com/5meodmt.html


you can also buy pure tryptamine from the same company for the low price of 99 dollars for 50 grams

http://www.goemerchant7.com/index.cgi?PageToView=catalog&Department=123021&Cartid=30801055798227&Merchant=JMCReSale&ExpandedDepts=113036

uneasy1


--------------------
anything i might say is just something i heard from the voices in my head
uneasy1


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Offlinebrainnoise
journeyman
Registered: 05/24/00
Posts: 69
Last seen: 19 years, 3 months
Re: Lets start fresh...DMT substrate [Re: Humidity]
    #1650097 - 06/21/03 08:58 AM (19 years, 3 months ago)

Would the DMT containing Grasses have to have the DMT extracted? Would it be easier to just grind up the grasses (or the root bark)
and add it to your substrate?
What would be a more sujective way of measuring the psylicyban content of the modified substrate over the control substrate?
I have thought of doing a basic chemistry extraction:
since psylocybes are water soluble,
you can make a tea concentrate with dry mushies only- water weighs too much and could significantly effect the data.
filter the resulting fluid and standardize
then dry it- this should leave a residue.

In theory, the "enhanced" substrate would leave more residue. would that work? or are the amounts to minute to give us significant results.

I am not sure what the necessity of adding enhancers to the mushies, alas the addition of any protein, I believe would enhance the psylocybian content of any mushy.


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Invisiblemicro
bunbun has a gungun
Male User Gallery

Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: Lets start fresh...DMT substrate [Re: brainnoise]
    #1650789 - 06/21/03 08:07 PM (19 years, 3 months ago)



--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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Offlineripper225
Stranger
Registered: 09/24/05
Posts: 16
Last seen: 15 years, 8 months
Re: Lets start fresh...DMT substrate [Re: micro]
    #4712121 - 09/26/05 07:36 AM (17 years, 2 days ago)

dirtmaster you tool AAHAHAA

so anyways, how'd this experiement turn out?


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Invisibleshymanta
Mad Scientist
 User Gallery

Registered: 01/27/05
Posts: 907
Re: Lets start fresh...DMT substrate [Re: ripper225]
    #4712296 - 09/26/05 08:10 AM (17 years, 2 days ago)

Another source of DMT is a toad venom. It has DMT, 5-MeO-DMT, and 5-HO-DMT in it. To what concentration, I don't know but it would be an abundant source if you could house the Bufo Alvarius toad. I have thought about putting some in substrate but have wondered about the heat in sterilization destroying the goodies.


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Offlinemuaddib
Stranger
Registered: 06/29/04
Posts: 2
Last seen: 17 years, 1 day
Re: Lets start fresh...DMT substrate [Re: shymanta]
    #4718498 - 09/27/05 11:10 AM (17 years, 1 day ago)

OK, since someone else dredged up this post, may I seek that those that participated in this circle of speakers in the (now) ancient past, convey any knowledge that they learned in this endeavor, or did you just  sit on the piles of shrooms you grew :smile:


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