Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   Original Sensible Seeds High THC Strains   OlympusMyco.com Olympus Myco Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblebwo
Stranger
Registered: 05/14/02
Posts: 17
grain transfers - maximum possible?
    #635309 - 05/18/02 09:23 AM (22 years, 4 months ago)

hi. tmc recommends no more than 3 grain transfers from an original master. although they say that "some" commercial operations get away with 4.

is this related to contams or senescence? i imagine it is contams although i have gotten away with 5...

anyone else have experience with this?


--------------------
"habit is the ballast that chains the dog to its vomit" - s beckett

Extras: Filter Print Post Top
Invisiblearmedia
Dikaryon

Registered: 11/03/01
Posts: 169
Re: grain transfers - maximum possible? [Re: bwo]
    #635355 - 05/18/02 10:21 AM (22 years, 4 months ago)

It has to do only with the increased possibility of contamination. In commercial systems, each transfer involves an exponential expansion of substrate mass (each jar can innoculate 10 jars, and so on), which means an exponential increase in contaminants, if they are introduced. Apparently 3 or 4 powers is the average that one can get away with, but if you're very careful, you might do better than that.

It has nothing to do with sensescence, which would only result from repeated transfers of the same strain, for many iterations, which can occur if you keep reculturing a strain on agar for years. However, this is a subject of some debate, and some people feel it only arises when a strain is cultured on media that with toxic components (such as caramelized sugars) or simple media that do not keep the mushroom enzymes active, and can be easily avoided by rotating media recipes often, so the mushroom sees different substrates often (kind of like excercising it.)

It's not really like generations (kids of kids of kids, etc.) since no sexual recombination is going on when you transfer from grain-to-grain. That would be more like germinating spores from a mushroom in each fruiting, which would be another way to avoid the possibility of senescence.

- A

Extras: Filter Print Post Top
Invisiblebwo
Stranger
Registered: 05/14/02
Posts: 17
Re: grain transfers - maximum possible? [Re: armedia]
    #636095 - 05/19/02 05:35 AM (22 years, 4 months ago)

haha so basically mushroom mycelium can live forever as long as you give it the proper (bi-annual?) exercise! i always wondered why senescence happened, it never made sense to me since mushrooms are a fungus...

cocnerning the grain transfers, i wonder how my sixth transfer will go in a week or so. its fun to push the envelope.


--------------------
"habit is the ballast that chains the dog to its vomit" - s beckett

Extras: Filter Print Post Top
Offlinehermes3
journeyman
Registered: 07/17/00
Posts: 56
Last seen: 13 years, 11 months
Re: grain transfers - maximum possible? [Re: bwo]
    #683477 - 06/17/02 08:19 AM (22 years, 3 months ago)

I've gone over a year with probably eight transfers grain to grain. I slightly vary my grain with yeast or dung or different season's stream water. This has for me been the limit before my crops start losing their vitality. I always back up with spore prints at exceptional flushes.

Extras: Filter Print Post Top
Invisibledurban_poison
myco contractor
Registered: 09/19/01
Posts: 2,417
Re: grain transfers - maximum possible? [Re: bwo]
    #683562 - 06/17/02 09:13 AM (22 years, 3 months ago)

fire ants I know havent germinated spores in a very long time always using grain to grain an infinite times. not a problem ever.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   Original Sensible Seeds High THC Strains   OlympusMyco.com Olympus Myco Bulk Substrate

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Open air grain to grain transfer idea..
( 1 2 all )		 Sorted 7,475 20 06/06/03 07:52 PM
by FungusmaximusFM
* Grain to grain transfer bag defunkt99 2,764 4 11/12/03 11:53 AM
by mycofile
* grain 2 1 month old grain transfer? bent 827 1 01/24/04 09:28 AM
by RockGut
* Will this work? (grain to grain and senescence) Anonymous 2,732 17 01/31/03 04:46 AM
by Azmodeus
* tissue and grain
( 1 2 all )		 La_Magdalene 4,139 32 02/01/03 01:42 PM
by La_Magdalene
* Peroxidated grains MickyWilliams 2,846 13 01/18/06 10:27 AM
by mycofile
* Grain 2 Grain... PooPs 1,025 2 06/05/03 12:37 PM
by PooPs
* Re: Chronic contams in transfers to wood Blue333 986 1 03/20/01 03:24 PM
by Suntzu

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte, bodhisatta
1,732 topic views. 1 members, 2 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.007 seconds on 14 queries.