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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 8 years, 6 months
Grain 2 Grain...
    #1611263 - 06/05/03 02:07 PM (14 years, 4 months ago)

when doing grain transfers....

is it best to use freshly colonized grain, or use the grain as close to the original clone tissue as possible!???

I have the jar that i used to transfer the agar from a clone tissue, wich was used to spawn 5 other quarts, lots left over, these 5 jars are colonized 100 % and could be used to transfer to more grain.. or i should i use the original jar???

does it make any difference?

opinions pls!


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Sniff, Sniff... What's that smell???... ohhhhh.!!
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Pot Free for another : nevermind.. never made it..


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Invisiblemycofile
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Registered: 01/19/99
Posts: 2,336
Loc: Uranus
Trusted Cultivator
Re: Grain 2 Grain... [Re: PooPs]
    #1611358 - 06/05/03 02:33 PM (14 years, 4 months ago)

Quote:

is it best to use freshly colonized grain, or use the grain as close to the original clone tissue as possible!???




The answer is yes :wink:

In your case, you could use either, but I'd say the freshest spawn would be the best.  You really only need to worry about using younger cell lines after you've had much more growth than either of your cases represent.  Like with a 4th generation grain jar for example.

Also, it makes some difference as to what you plan to do with the grain, case it or use it as spawn.  If you use it as spawn, what is your spawn rate going to be?  These things will affect the total number of cell divisions before you reach a fruiting stage.  Basically, the more bulk substrate you plan to colonize, the more important the mycelium's distance from the original culture is.

Since second and even third generation grain jars (1000+X the mass of the original jar) are perfectly capable of colonizing several tons of bulk substrate, you are no where near close to having to worry about anything.  At least I hope not!


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"From a certain point of view"
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OfflinePooPs
What's thisfor???

Registered: 03/05/03
Posts: 4,510
Loc: Stirrin up the pile!
Last seen: 8 years, 6 months
Re: Grain 2 Grain... [Re: mycofile]
    #1611381 - 06/05/03 02:37 PM (14 years, 4 months ago)

cool.

I spawn about 1:6 ratio of storebaught cow poop and straw!.

TONS... my god.. i wish!


--------------------


-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


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Amazon Shop: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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