Home | Community | Message Board

This site includes paid links. Please support our sponsors.

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Registered: 01/02/01
Posts: 11
Last seen: 21 years, 10 months
Re: Chronic contams in transfers to wood
    #274882 - 03/20/01 10:58 AM (22 years, 2 months ago)

I'm reasonably sure that I isolated pure mycelium. I took some vigorous rhizomorphic threads, blended them up in a peroxide solution, then injected into grain. Several jars contaminated, but I finally got down to three that colonized for about a month or so with no visible contams. From there, I've made more grain and then mycelium syringes and grain transfers to all the varieties of wood I mentioned.

Interestingly, I kept the wild mycelium in a plastic bin outside, supplementing it with cardboard and paper bag material. It took to that reasonably well, albeit slowly. Introduced to cardboard indoors, I got Trich. That might say something about the nature of my indoor air right there.

Though I have Stamets' TMC, I haven't read about such radical sterilization of wood as 4-5 hours 15 psi. Pages 110-111 recommend fresh alder, outside bed, no sterilizing. Natural competitor selectivity clearly plays a role here, and might be worth trying.

In all the failures, there is a single jar left: PF-style cake of pet shop Aspen wood and bran. Been going for three weeks, no contams yet. I cross my fingers every day for it. What would you recommend to be the next step I take with that? Try a cardboard transfer outside? Alder again after 5 hours PC? I have some colonized agar in the fridge, so I'll always have something to fall back on, but I am starting to dislike this whole falling business.

Your post and your assistance are appreciated. Thanks for responding.

Extras: Filter Print Post Top
 User Gallery

Registered: 10/14/99
Posts: 1,396
Last seen: 11 months, 12 days
Re: Chronic contams in transfers to wood [Re: Blue333]
    #275407 - 03/20/01 03:24 PM (22 years, 2 months ago)

My TMC is with a friend, but my old GGMM [1st ed?] talks about it on page 167. Of course 4-5 hrs @ 15 PSI is the recommendation for indoor, supplemented woodchips. 2-3 if unsupplemented.

You might just have a randomly high contam spore count.

From agar onto molasses-supplemented chips, I see renewed growth within 2 days. Last batch was simmered for about 3 hours, then PC'd ~10 PSI, 2 hrs.

One other trick that helped me once with a cyanescens transplant--I had only a couple of colonized chips, placed them within new chips and covered loosely with soil. Kept the container outdoors and misted/moist. I think it colonized quicker than it would have if it were woodchips alone.

Hope yours gives a great flush this fall;

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures

Similar ThreadsPosterViewsRepliesLast post
* Beer soaked cardboard
( 1 2 3 all )
zeronio 13,014 43 01/12/04 10:29 PM
by Yarry
* need help for cardboard technique jekojeko 2,320 7 08/24/02 09:57 AM
by tchyted
* Cardboard cloning tek?
( 1 2 all )
ink 25,704 31 02/14/16 10:43 PM
by Eywa_devotee
* cardboard replacement for agar? Imorph 3,078 3 11/28/01 01:09 AM
by srivatsa
* Experimental Wood Loving Agar Media maxphun 3,167 9 10/24/02 08:59 AM
by comario2
* Preparing Cardboard and Wood Substrate retread 3,007 7 10/30/04 02:55 AM
by mycoguy
* Cardboard as substrate StopThat 2,642 7 03/03/03 05:19 AM
by StopThat
* Agar: Malt concentrations and supplements... ATWAR 2,854 7 07/04/04 08:20 PM

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
977 topic views. 0 members, 3 guests and 0 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2023 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.007 seconds on 14 queries.