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Offlinezeronio
Stranger
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Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 6 days, 22 hours
Re: Beer soaked cardboard [Re: stefan]
    #884289 - 09/15/02 08:37 PM (22 years, 5 months ago)

I used a very high concentration (1.5%, like 1l of 3% H2O2 and 1l of H2O ), but that might be unnecessary. I have to do more experiments...
I have to mention that bacterial contamination is hard to notice on cardboard.

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Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 18 years, 4 months
Re: Beer soaked cardboard [Re: zeronio]
    #1222338 - 01/15/03 10:00 PM (22 years, 1 month ago)

hey zeronio

i am not in advanced forum so i just saw your post :smile:

i have done this with boiled water and cardboard, nothing more. no nutrients no anything.

imho, its the safest method to send myc over the snail-mail
this is how i am gonna send you the myc we were talking. the agar plates took a post walk and got contaminated lol lol so i am gonna send you on cardboard

actually noone cares if it has any nutrients if this method is for transfering myc. the only thing you care is to send it healthy. its so easy then to throw it in a jar of rye or to put it in a petri dish with agar as normal. :smile: 


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The living ghost of Kykeon

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Offlinezeronio
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Registered: 10/16/01
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Re: Beer soaked cardboard [Re: kykeon]
    #1222360 - 01/15/03 10:27 PM (22 years, 1 month ago)

I used this for a short period of time when I ran out of agar. It's major drawback is that bacterial contaminants are hard to see.
Quote:

imho, its the safest method to send myc over the snail-mail



Let's try it. It should be OK.

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Offlinedumlovesyou
retired shroomer
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Registered: 05/02/01
Posts: 688
Loc: One dimension
Last seen: 2 years, 9 months
Re: Beer soaked cardboard [Re: zeronio]
    #1222394 - 01/15/03 11:16 PM (22 years, 1 month ago)

What do you mean by saying you cannot see the contams..It looks pretty noticeble.


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I see trees of green, psylocibe mushrooms too
I see them bloom for me and you
And I think to myself what a wonderful world

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Offlinezeronio
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Loc: Slovenia
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Re: Beer soaked cardboard [Re: dumlovesyou]
    #1222430 - 01/15/03 11:56 PM (22 years, 1 month ago)

Agar has smooth surface and bacterial colonies are easier to spot.
I had contaminated cardbord plates that seemed OK, but the smell wasn't.

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Invisiblewoodrow
journeyman
Registered: 03/17/03
Posts: 142
Re: Beer soaked cardboard [Re: zeronio]
    #1419372 - 03/31/03 04:54 AM (21 years, 10 months ago)

I once tried using beer as an agar ingredient and my agar wouldn't grow shit. Some beers contain powerful preservatives. German beers are preservative free but cardboard agar seems a waste of gut deutch Bier. Brewing your own may be the way to go.

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OfflineBig_D
Baaith AKA LoneSoldier
Registered: 04/11/03
Posts: 50
Last seen: 19 years, 5 months
Re: Beer soaked cardboard [Re: zeronio]
    #1466787 - 04/17/03 09:36 AM (21 years, 10 months ago)

When you said you innoculated the cardboard...

Did you rub spors over it? or did you inject the cardboard with a spore syringe?

How long did you pressure cook the cardboard?


I've always PDA or MEA, but this method seems easir and will produce less contaminants

peace,


--------------------
Leave Me Alone!

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InvisibleGGreatOne234
Stranger
Registered: 12/23/99
Posts: 8,946
Re: Beer soaked cardboard [Re: zeronio]
    #1473156 - 04/19/03 12:21 PM (21 years, 10 months ago)


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Offlinezeronio
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Re: Beer soaked cardboard [Re: Big_D]
    #1473247 - 04/19/03 01:05 PM (21 years, 10 months ago)

I didn't try to germinate spores on it - I used this when I ran out of agar and transfered mycellium from agar plates. Rush Wayne (peroxide manual) proposes method of germinating spores on cardboard without beer (& without peroxide of course) - it has less nutrients this way so there is less chance of contamination. As soon as you get some growth you transfer mycellium to peroxidated plates.

I'm quite surprised that this thread still gets attention. :cool: I will try it again and make a pictorial guide to it. 

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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
Re: Beer soaked cardboard [Re: zeronio]
    #1836427 - 08/21/03 07:27 AM (21 years, 6 months ago)

any updates, zeronio ?


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Admin @ mycotopia.net
Mycotopia

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Anonymous

Post deleted by papaver [Re: Hippie3]
    #1838583 - 08/21/03 07:21 PM (21 years, 6 months ago)


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Offlinepsiloz
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Registered: 06/03/03
Posts: 75
Loc: Australia
Last seen: 19 years, 9 months
Re: Beer soaked cardboard [Re: ]
    #1842000 - 08/22/03 08:05 PM (21 years, 5 months ago)

I used cardboard successfully to germinate woodlover spores.
Sterilise cradbroard. cool. use forceps to touch moist cardoard to print and place face up on peroxide agar - works great.

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Offlinescreaminsemen
enthusiast

Registered: 07/28/03
Posts: 308
Last seen: 21 years, 3 months
Re: Beer soaked cardboard [Re: psiloz]
    #1843217 - 08/23/03 11:40 AM (21 years, 5 months ago)

Could you use beer instead of water in making cakes?

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Offlinemattch1
Monseur

Registered: 05/16/03
Posts: 126
Loc: WV
Last seen: 20 years, 5 months
Re: Beer soaked cardboard [Re: screaminsemen]
    #1854198 - 08/27/03 08:22 AM (21 years, 5 months ago)

Im currently trying Karo soaked cardboard as a variation of this technique. Ive been innoculating from a liquid culture (Karo + water) that I made, but it hasnt shown any growth yet. I will keep you posted on the success/failure.


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Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.

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Offlinecomario2
amateur

Registered: 09/06/02
Posts: 1,352
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Re: Beer soaked cardboard [Re: mattch1]
    #1854869 - 08/27/03 12:47 PM (21 years, 5 months ago)

Quote:

mattch1 said:
Im currently trying Karo soaked cardboard as a variation of this technique. Ive been innoculating from a liquid culture (Karo + water) that I made, but it hasnt shown any growth yet. I will keep you posted on the success/failure.


in my experience, the cardboard tek works better if you moisten the cbd with sterile water, without any karo, beer or other nutes, like this


--------------------
comario


"crusaders against emotional poverty"

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Offlinecomario2
amateur

Registered: 09/06/02
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Re: Beer soaked cardboard [Re: comario2]
    #1854883 - 08/27/03 12:51 PM (21 years, 5 months ago)

and then   :smile: 


--------------------
comario


"crusaders against emotional poverty"

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Offlinemattch1
Monseur

Registered: 05/16/03
Posts: 126
Loc: WV
Last seen: 20 years, 5 months
Re: Beer soaked cardboard [Re: comario2]
    #1862113 - 08/29/03 08:17 AM (21 years, 5 months ago)

do u use mycelium from an agar medium to colonize the cardboard?
~MC


--------------------
Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.

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Offlinecomario2
amateur

Registered: 09/06/02
Posts: 1,352
Loc: between places
Last seen: 12 years, 1 month
Re: Beer soaked cardboard [Re: mattch1]
    #1862173 - 08/29/03 08:48 AM (21 years, 5 months ago)

no, that would defy the purpose of this tek. you germinate the spores on a tiny piece of cbd, which is placed on peroxidated mea


--------------------
comario


"crusaders against emotional poverty"

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Offlinemattch1
Monseur

Registered: 05/16/03
Posts: 126
Loc: WV
Last seen: 20 years, 5 months
Re: Beer soaked cardboard [Re: comario2]
    #2232510 - 01/09/04 11:35 PM (21 years, 1 month ago)

ehhh... cardboard isnt nutritious, are u sure u dont have a woodlover there?


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Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.

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OfflineTheOneYouKnow
addict
Registered: 01/04/04
Posts: 470
Last seen: 20 years, 10 months
Re: Beer soaked cardboard [Re: mattch1]
    #2232546 - 01/09/04 11:51 PM (21 years, 1 month ago)

I've seen pictures from Psilocybe Mushrooms around the World, and maybe GGMM (I forget, I'll find the URL's for them) that show p. cubensis fruiting off of newspaper, works pretty well


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Opinions are like assholes; everyone needs one or else they'd explode

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Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract


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