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Invisiblemaxphun
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Registered: 09/25/02
Posts: 32
Experimental Wood Loving Agar Media
    #906960 - 09/25/02 11:37 AM (14 years, 2 months ago)

Hi All

Experiments are currently in progress to use cardboard agar for wood loving mushrooms.

To make cardboard solution:

Cut up cardboard into small peaces..
Leave it sitting in 1 L of water for 24 hours..
Mix up in an high speed electric blender..

This leaves cardboard solution

Add and mix: 20 grams agar to the Cardboard solution.

Pressure cook for 35 min and use as standard agar.

No results are available as experiments are in progress. If anyone else has tried this or similar agar media please let me know.




Potentially a low contamination medium for multispore culture?

Thanks 

***MaxPhun***  :cool:
 


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OfflineDERRAYLD
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Re: Experimental Wood Loving Agar Media [Re: maxphun]
    #907027 - 09/25/02 12:39 PM (14 years, 2 months ago)

Sounds like an idea, except how exactly will you see the contamination if it actually starts growig?
That`s a lump of brown goo.


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Invisiblemaxphun
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Registered: 09/25/02
Posts: 32
Re: Experimental Wood Loving Agar Media [Re: DERRAYLD]
    #907127 - 09/25/02 01:48 PM (14 years, 2 months ago)

Yes good point.

Does the lower chance of contamination out weigh any difficulties in spotting contamination?

I am not sure!

A magnifying glass will help





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InvisibleRoadkill
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Re: Experimental Wood Loving Agar Media [Re: maxphun]
    #907963 - 09/25/02 08:27 PM (14 years, 2 months ago)

I actually like this idea.

I'd like to see you post the actual ammount of cardboard used.

like in grams.

thanks!





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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineDinoMyc
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Re: Experimental Wood Loving Agar Media [Re: maxphun]
    #908018 - 09/25/02 08:54 PM (14 years, 2 months ago)

Staments reccomends adding fine sawdust to agar for lignacious fungi.
Please keep us posted.


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If I made affirmation, I apologize.
I merely came to listen, came to say.


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Offlinecomario
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Re: Experimental Wood Loving Agar Media [Re: DinoMyc]
    #908072 - 09/25/02 09:21 PM (14 years, 2 months ago)

i like the sawdust idea better than the cboard, anyway i'd like to see a comparison, maybe i'll give it a shot


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OfflineOrchidman
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Re: Experimental Wood Loving Agar Media [Re: maxphun]
    #909231 - 09/26/02 07:05 AM (14 years, 2 months ago)

My opinion is that the contamination will still come because of the agar. I am growing Psilocybe cyanescens and my experience is that they don't like the cardboard. They grew very slowly on the cardboard compared to the Aspen Shavings Pet Litter. Because I made the mistake of starting off with a lot of cardboard and a small amount of mycelium, before the growth could take over the entire cardboard it contaminated with grey mold at the edges. To say that cardboard doesn't contaminate is an error. I eliminated the cardboard and have had great success. I don't know why anyone would grow wood eaters on agar anyway. I started off with syringes into Sugar Maple Sawdust and Wheat Bran in Jelly Jars. This grew rapidly and sturdily and once they had consumed the entire jar I buried it whole into a clear Glad container. Once the bran is consumed, there is no need for any other sterile proceedures.

The jelly jars cakes were burried whole into soaked (in hot tap water), not sterilized Aspen shavings. I used Kaytee brand from Petsmart. It took a couple of weeks for the jars to move into the shavings, but then look out. The growth was strong and rapid. You can see right into the glad containers and here is the part where you section out the best growth. If you like you can just dig out a chunk of the best growth with a sterile fork and put it by itself into another Glad container of Aspen. Don't mess with the original jar as it has the bran in it and might contaminate if you expose it.The new chunk will continue to grow strong and sturdy and maintain the strong growth. As I said, there is no need for agar. Agar just gets contaminated.

Once the Glad container is full I transplanted it into a dark Rubbermaid tote full of shavings. One tote I chopped the cake up and mixed it into the shavings and the other I burried whole. Both ended up about tied for full collonization but the one that was burried whole has much stronger growth. These mushrooms are like pets and you have to feed them. I started off with 9 litres in the tote or about 2 gallons. After a month or so, I noticed that it was shrinking down to about 5 litres. I realized the mushrooms had consumed the wood. I put about 5 litres of soaked shavings on top and now after a month those have been consumed. I keep having to feed them, and they love it. I hate to think that I"m going to have to put them outside for the winter and they might not be able to survive our cold. I bought a little refrigerator just for them and I'm going to try to fruit them indoors. After all, nothing is too good for my pets. I even talk to them sometimes.


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Invisiblemaxphun
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Posts: 32
Re: Experimental Wood Loving Agar Media [Re: Orchidman]
    #909387 - 09/26/02 11:11 AM (14 years, 2 months ago)

I used about 20 grams of dry cardboard...

I used agar for isolating a pure strain from a spore print that could be contaminated.

Orchidman, if the print was known to be clean I would use your suggested method, but as it could be contaminated, growing on agar seems the safest bet. I have read your posts on growing Psilocybe cyanescens and found them very informative (thanks).

Note..Stamets & Chilton (1983) show a picture of Psilocybe cyanescens showing some impressive rhizomorphic growth on cardboard.

This is just an experiment and I have not put all my eggs in one basket. I also have some standard MEA to use and other experimental media to try.


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Edited by maxphun (09/26/02 11:13 AM)


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Invisiblemaxphun
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Posts: 32
Re: Experimental Wood Loving Agar Media [Re: maxphun]
    #987165 - 10/23/02 06:10 PM (14 years, 1 month ago)

No growth was observed  :frown:  and no contamination. 


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Offlinecomario2
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Re: Experimental Wood Loving Agar Media [Re: maxphun]
    #989123 - 10/24/02 10:59 AM (14 years, 1 month ago)

try the sawdust, and use way less, just a spec per plate


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comario


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