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zeronio
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Beer soaked cardboard
#742923 - 07/12/02 09:25 PM (21 years, 8 months ago) |
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Here is an experiment I made with a variation of cardboard substitue for agar: - boil beer for a minute to let some alcohol out. - soak corrugated cardboard in it and put it in glass petri dish - pressure sterilize - I sprayed some H2O2 on it before inoculation
And here are the results after 1 week:
Some dishes dried too much during sterilization so next time I will wrap them in aluminium foil.
Psilocybe cubensis Golden Teacher
The interesting thing is that mycellium grows also on the underside:
Psilocybe cubensis Koh Samui Note that this one was too dry and mycellium grew inside cardboard and popped out on the upper left corner.
Pleurotus djamor Dish is fully overgrown it only looks a bit thin compared to standard agar cultures. However I prefer this one because this strain on agar prefers to form fruitbodies than spreading around.
Flammulina velutipes This is a bit too aerial for my taste (probably because cardboard is too dry).
Next time I will try to germinate spores on it. What do you think?
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DinoMyc
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Re: Beer soaked cardboard [Re: zeronio]
#743135 - 07/13/02 04:21 AM (21 years, 8 months ago) |
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interesting. It should work best for wood lovers, as the cardboard itself is otherwise wasted/just a medium.
-------------------- If I made affront, I apologize. If I made affirmation, I apologize. I merely came to listen, came to say.
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Macey Howard
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Post deleted by Moe Howard [Re: zeronio]
#743157 - 07/13/02 04:48 AM (21 years, 8 months ago) |
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-------------------- Hugs and Kisses!
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Anonymous
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Re: Beer soaked cardboard [Re: Macey Howard]
#743277 - 07/13/02 06:51 AM (21 years, 8 months ago) |
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very cool. i may have to try this if i lose access to my agar supply.
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zeronio
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Re: Beer soaked cardboard [Re: DinoMyc]
#743338 - 07/13/02 07:34 AM (21 years, 8 months ago) |
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"It should work best for wood lovers, as the cardboard itself is otherwise wasted/just a medium. "
Yes, for cubes it's just a sponge containing nutrients.
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Suntzu
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Re: Beer soaked cardboard [Re: zeronio]
#743468 - 07/13/02 09:13 AM (21 years, 8 months ago) |
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EXCELLENT. What else can be said. Beer is an incredibly complex medium, and obviously seems to work. Congrats, the beer-cardboard agar-substitution will make pure culture accessible to just about anyone [unless you're under 21 ]
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StInvetroThomas
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Re: Beer soaked cardboard [Re: Suntzu]
#743931 - 07/13/02 03:05 PM (21 years, 8 months ago) |
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However...the cubensis looks like it kind of likes it...maybe not as anything but a vessel for growth, but I could see it being good maybe to inoculate an outdoor cow patty..maybe someone should try it.
-------------------- "...I found dozens of single specimens. That's what I call hunting. There are only a few "good" hunters here, even now. You're certainly in that group. I would imagine if we hunted together we'd find our styles are similar." - Mr. Mushrooms RIP Matt, your friendship and your contributions to the world of fungi will be missed. Unfortunately we never got to hunt together. St Thomas
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DinoMyc
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Re: Beer soaked cardboard [Re: zeronio]
#743963 - 07/13/02 03:34 PM (21 years, 8 months ago) |
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I allmost forgot to mention... what of the poor beer? wouldn't it rather be drunk? just a thought
-------------------- If I made affront, I apologize. If I made affirmation, I apologize. I merely came to listen, came to say.
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ChromeCrow
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Re: Beer soaked cardboard [Re: zeronio]
#746052 - 07/14/02 05:30 PM (21 years, 8 months ago) |
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In reply to:
Next time I will try to germinate spores on it. What do you think?
if you leave off the h2o2, it will work fine, i have had great success germinating spores on cardboard simply wetted and microwaved, then placed spore side up on peroxide agar plates. the cardboard acts as a barrior from the peroxide in the agar, and once the myc reaches it, it takes off like you wouldnt believe. i had a fellow pm me, trying this out, but he used a dextrose solution to soak the cardboard in, and got contamination. i have had 0 contams using straight cardboard/water
-------------------- ISO: Orissa, Malabar, z strain
Edited by ChromeCrow (08/24/02 03:52 PM)
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zeronio
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Re: Beer soaked cardboard [Re: ChromeCrow]
#746790 - 07/14/02 09:45 PM (21 years, 8 months ago) |
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Sound like a good idea. It's better to germinate spores on less nutrient medium to avoid contamination.
I found out that cardboard is drying out very quickly compared to agar. I use Micropore medical tape to seal petris and it seems that it lets too much moisture out. Agar is hard to beat.
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vatoloco
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Post deleted by MOE THE MAD SCIENTIST [Re: ChromeCrow]
#750716 - 07/16/02 09:59 AM (21 years, 8 months ago) |
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Anonymous
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Re: Beer soaked cardboard *DELETED* [Re: vatoloco]
#762992 - 07/20/02 06:19 PM (21 years, 7 months ago) |
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Post deleted by sneakybastage420
Edited by sneakybastage420 (07/20/02 06:26 PM)
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zeronio
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Re: Beer soaked cardboard [Re: ]
#816098 - 08/13/02 07:30 PM (21 years, 7 months ago) |
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No I think there should be no problem, the only thing is that maybe you will need a filter patch on the lid.
I used this BSC a couple of times and I found that the biggest problem is that it is very difficult to cut wedges out of cardboard. Next time I will cut it to small pieces before sterilization and inoculation.
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DETACHABLE_PENIS
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Re: Beer soaked cardboard [Re: zeronio]
#856254 - 09/01/02 05:53 AM (21 years, 6 months ago) |
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im curious, what would the next step be. inoculate jars or colonize substrate?
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DERRAYLD
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Most neetest thing hey Moe? Lol Nice experiment. That would most likely be used to innoculate a jar of grain. That wouldn`t be enough to spawn unless ofcourse the spawn was a tiny amount and you once again used it to spawn and then again and again. Too much effort.
Edited by DERRAYLD (09/01/02 07:37 PM)
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zeronio
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I used it to inoculate grain jars.
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tps
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Re: Beer soaked cardboard [Re: zeronio]
#858889 - 09/02/02 11:48 AM (21 years, 6 months ago) |
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ah so lite beer is good for something.
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psychomycul
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Re: Beer soaked cardboard [Re: zeronio]
#880339 - 09/12/02 10:25 AM (21 years, 6 months ago) |
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Once the the mycelial growth is done. What is the best to transfer to a substrate jars, bucket, outside etc. primarily Jars
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zeronio
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Re: Beer soaked cardboard [Re: psychomycul]
#880679 - 09/12/02 01:37 PM (21 years, 6 months ago) |
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IME you can transfer wedges to fresh petris to keep it going or inoculate substrate jars.
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stefan
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Re: Beer soaked cardboard [Re: zeronio]
#883556 - 09/14/02 06:28 AM (21 years, 6 months ago) |
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How much 3% H2O2 do I have to add to a liter of water to get a good strength for spraying over those petri's?
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zeronio
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Re: Beer soaked cardboard [Re: stefan]
#884289 - 09/15/02 08:37 PM (21 years, 6 months ago) |
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I used a very high concentration (1.5%, like 1l of 3% H2O2 and 1l of H2O ), but that might be unnecessary. I have to do more experiments... I have to mention that bacterial contamination is hard to notice on cardboard.
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kykeon
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Re: Beer soaked cardboard [Re: zeronio]
#1222338 - 01/15/03 10:00 PM (21 years, 2 months ago) |
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hey zeronio
i am not in advanced forum so i just saw your post
i have done this with boiled water and cardboard, nothing more. no nutrients no anything.
imho, its the safest method to send myc over the snail-mail this is how i am gonna send you the myc we were talking. the agar plates took a post walk and got contaminated lol lol so i am gonna send you on cardboard
actually noone cares if it has any nutrients if this method is for transfering myc. the only thing you care is to send it healthy. its so easy then to throw it in a jar of rye or to put it in a petri dish with agar as normal.
-------------------- The living ghost of Kykeon
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zeronio
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Re: Beer soaked cardboard [Re: kykeon]
#1222360 - 01/15/03 10:27 PM (21 years, 2 months ago) |
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I used this for a short period of time when I ran out of agar. It's major drawback is that bacterial contaminants are hard to see.
Quote:
imho, its the safest method to send myc over the snail-mail
Let's try it. It should be OK.
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dumlovesyou
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Re: Beer soaked cardboard [Re: zeronio]
#1222394 - 01/15/03 11:16 PM (21 years, 2 months ago) |
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What do you mean by saying you cannot see the contams..It looks pretty noticeble.
-------------------- I see trees of green, psylocibe mushrooms too I see them bloom for me and you And I think to myself what a wonderful world
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zeronio
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Re: Beer soaked cardboard [Re: dumlovesyou]
#1222430 - 01/15/03 11:56 PM (21 years, 2 months ago) |
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Agar has smooth surface and bacterial colonies are easier to spot. I had contaminated cardbord plates that seemed OK, but the smell wasn't.
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woodrow
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Re: Beer soaked cardboard [Re: zeronio]
#1419372 - 03/31/03 04:54 AM (20 years, 11 months ago) |
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I once tried using beer as an agar ingredient and my agar wouldn't grow shit. Some beers contain powerful preservatives. German beers are preservative free but cardboard agar seems a waste of gut deutch Bier. Brewing your own may be the way to go.
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Big_D
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Re: Beer soaked cardboard [Re: zeronio]
#1466787 - 04/17/03 09:36 AM (20 years, 11 months ago) |
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When you said you innoculated the cardboard...
Did you rub spors over it? or did you inject the cardboard with a spore syringe?
How long did you pressure cook the cardboard?
I've always PDA or MEA, but this method seems easir and will produce less contaminants
peace,
-------------------- Leave Me Alone!
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GGreatOne234
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Re: Beer soaked cardboard [Re: zeronio]
#1473156 - 04/19/03 12:21 PM (20 years, 10 months ago) |
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zeronio
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Re: Beer soaked cardboard [Re: Big_D]
#1473247 - 04/19/03 01:05 PM (20 years, 10 months ago) |
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I didn't try to germinate spores on it - I used this when I ran out of agar and transfered mycellium from agar plates. Rush Wayne (peroxide manual) proposes method of germinating spores on cardboard without beer (& without peroxide of course) - it has less nutrients this way so there is less chance of contamination. As soon as you get some growth you transfer mycellium to peroxidated plates.
I'm quite surprised that this thread still gets attention. I will try it again and make a pictorial guide to it.
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Hippie3
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Re: Beer soaked cardboard [Re: zeronio]
#1836427 - 08/21/03 07:27 AM (20 years, 6 months ago) |
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any updates, zeronio ?
-------------------- Admin @ mycotopia.net Mycotopia
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Anonymous
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Post deleted by papaver [Re: Hippie3]
#1838583 - 08/21/03 07:21 PM (20 years, 6 months ago) |
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psiloz
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Re: Beer soaked cardboard [Re: ]
#1842000 - 08/22/03 08:05 PM (20 years, 6 months ago) |
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I used cardboard successfully to germinate woodlover spores. Sterilise cradbroard. cool. use forceps to touch moist cardoard to print and place face up on peroxide agar - works great.
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screaminsemen
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Re: Beer soaked cardboard [Re: psiloz]
#1843217 - 08/23/03 11:40 AM (20 years, 6 months ago) |
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Could you use beer instead of water in making cakes?
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mattch1
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Im currently trying Karo soaked cardboard as a variation of this technique. Ive been innoculating from a liquid culture (Karo + water) that I made, but it hasnt shown any growth yet. I will keep you posted on the success/failure.
-------------------- Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.
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comario2
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Re: Beer soaked cardboard [Re: mattch1]
#1854869 - 08/27/03 12:47 PM (20 years, 6 months ago) |
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Quote:
mattch1 said: Im currently trying Karo soaked cardboard as a variation of this technique. Ive been innoculating from a liquid culture (Karo + water) that I made, but it hasnt shown any growth yet. I will keep you posted on the success/failure.
in my experience, the cardboard tek works better if you moisten the cbd with sterile water, without any karo, beer or other nutes, like this
-------------------- comario "crusaders against emotional poverty"
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comario2
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Re: Beer soaked cardboard [Re: comario2]
#1854883 - 08/27/03 12:51 PM (20 years, 6 months ago) |
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and then
-------------------- comario "crusaders against emotional poverty"
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mattch1
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Re: Beer soaked cardboard [Re: comario2]
#1862113 - 08/29/03 08:17 AM (20 years, 6 months ago) |
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do u use mycelium from an agar medium to colonize the cardboard? ~MC
-------------------- Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.
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comario2
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Re: Beer soaked cardboard [Re: mattch1]
#1862173 - 08/29/03 08:48 AM (20 years, 6 months ago) |
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no, that would defy the purpose of this tek. you germinate the spores on a tiny piece of cbd, which is placed on peroxidated mea
-------------------- comario "crusaders against emotional poverty"
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mattch1
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Re: Beer soaked cardboard [Re: comario2]
#2232510 - 01/09/04 11:35 PM (20 years, 2 months ago) |
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ehhh... cardboard isnt nutritious, are u sure u dont have a woodlover there?
-------------------- Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.
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TheOneYouKnow
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Re: Beer soaked cardboard [Re: mattch1]
#2232546 - 01/09/04 11:51 PM (20 years, 2 months ago) |
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I've seen pictures from Psilocybe Mushrooms around the World, and maybe GGMM (I forget, I'll find the URL's for them) that show p. cubensis fruiting off of newspaper, works pretty well
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amyloid
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Re: Beer soaked cardboard [Re: mattch1]
#2233024 - 01/10/04 10:18 AM (20 years, 2 months ago) |
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i thought cardboard had nutrients in it, it is just mashed up trees isnt it?
-------------------- "A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty." -Al Einstein
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zeronio
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Re: Beer soaked cardboard [Re: amyloid]
#2236092 - 01/12/04 01:04 AM (20 years, 2 months ago) |
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Cardboard is supposed to be nutrient only for primary decomposers. I added beer because cubes are secondary decomposers and I thought they wouldn't grow only on soaked cardboard, but I after some time I saw that cubes grow on almost everything. Someone (I think that it was Joshua) fruited them on sawdust.
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Joshua
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Re: Beer soaked cardboard [Re: zeronio]
#2238022 - 01/12/04 09:45 PM (20 years, 2 months ago) |
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STAMETS, PAUL; CHILTON, J S Growing Parameters for Psilocybe cubensis (5) The Mushroom Cultivator: A Practical Guide to Growing Mushrooms at Home; Agarikon Press, Olympia WA; Chapter IX, p 196-203
COMMENTS: One of the easiest mushrooms to grow, this species fruits on a wide variety of substrates within broad environmental parameters. As a primary and secondary decomposer, Psilocybe cubensis fruits well on untreated pasteurized straw and on horse manure/straw composts transformed by microbial activity. Sterilized grain typically produces smaller mushrooms than bulk substrates. Given the numerous substrates that support fruitings, Psilocybe cubensis is well suited for home cultivation. Psilocybe cubensis cultivation was unheard of twenty years ago. Today, this species ranks among one of the most commonly cultivated mushrooms in the US and soon the world. This sudden escalation in interest is largely due to the publication of several popular guides illustrating techniques for its culture. Psilocybe cubensis is a mushroom with psychoactive properties, containing up to 1% psilocybin and/or psilocin per dried gram. The function of these serotonin-like compounds in the life cycle of the mushroom is not known
Joshua
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Yarry
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Re: Beer soaked cardboard [Re: Joshua]
#2238081 - 01/12/04 10:29 PM (20 years, 2 months ago) |
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wait.. i can grow mushrooms.. off of beer? *weeps with joy* now if you wanted to be like me, you'd use the cardboard from the beer box! lol. woot!
-------------------- Grumpy Old Man.
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