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Offlinedefunkt99
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Registered: 08/02/03
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Grain to grain transfer bag
    #2093961 - 11/11/03 09:42 PM (14 years, 1 month ago)

Let's say I had a quart of colonized corn and wanted to make 4-8 more out of them using the grain to grain transfer method, what would be the way to do this without having to make new quart jars again.

Is there a way to do this without using jars, possibly by using an
oven bag.

Any suggestions?


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Invisiblemicro
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Registered: 05/09/03
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Re: Grain to grain transfer bag [Re: defunkt99]
    #2094128 - 11/11/03 10:26 PM (14 years, 1 month ago)

You need to make new jars of substrate, anyway. The easiest way I've found is in bags -- if you have mycelium in bags you can just inject sterile water (do it with rubbing alcohol, etc., to keep it sterile) into the bag, and mix it in with some mycelium, and draw it (sterilly) back out with a sterile syringe. Just try not to let the water touch the big block of mycelium, or else it will absorb it and you'll be left with a very little amount of inoculum. You might be able to do this with jars, but it would probably be a pain-in-the-ass.

For the regular G2G tek you really need a glovebox that you sterilized beforehand -- there's a really big contamination factor since the stuff comes in contact with so much air.

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Offlinedefunkt99
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Registered: 08/02/03
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Last seen: 12 years, 6 months
Re: Grain to grain transfer bag [Re: micro]
    #2094295 - 11/11/03 11:03 PM (14 years, 1 month ago)

Thanks, Micro.

I'm not sure I'm following you here. I have a quart jar of colonized corn and don't want to PC new jars because my 4 quart cooker won't fit quart jars. I thinking of PCing a 4 quart oven bag filled with corn and then adding transferring the mycelium into the bag.

Is this workable? How would I do this?

Thanks again.


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Invisiblemicro
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Re: Grain to grain transfer bag [Re: defunkt99]
    #2094313 - 11/11/03 11:05 PM (14 years, 1 month ago)

No reason you can't do this, as long as you prepare the corn, of course. Just do it sterilly. You can't open bags or jars with substrate in an unsterile environment.

--
Micro


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Invisiblemycofile
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Re: Grain to grain transfer bag [Re: micro]
    #2096965 - 11/12/03 01:53 PM (14 years, 1 month ago)

Oven bags suck, you need an autoclavable bag made for this purpose. I don't know of a commonly available bag that would suit this purpose other than those made for mushroom cultivation. Oven bags just leak, which means contams get in and you're fucked. Even when using a real autoclave bag, heed micro's advice on sterility and use a glove box or flowhood.

If you really want to try it with an oven bag, coat the corn with a small amount of 3% peroxide after it's been pced. Search for info on how to get the right amount, but my understanding of corn is that it may be problmatic as corn doesn't really soak up the peroxide like other grains do. And you need to rig up a filter to that oven bag, I assume you know how to do that?


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