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He has taken things like DPT or DIPT and put them in the growth media and the fruiting bodies that came out contain 4-hydroxy-DPT or 4-hydroxy-DIPT instead of psilocin.

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This way you avoid a 10 step synthesis by growing a psychoactive mushroom that contains no illegal drug.

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These two comments seem to indicate that the compound psilocin was NOT produced, that the mycelia were instead hydroxylating the 5-MeO-DMT to produce 4,5,HO-Meo-DMT. I'm wondering if their is some heirarchy of compounds that the mycelia deals with in a specific order. It would also be possible that the amount of enzymes required to catalyze this reaction would be limited. Thus, you'd end up with the same amount of 4,5-HO-MeO-DMT, as you would psilocin, because the power for the conversion would have just run out.

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Well there's this,


Seems in both of the studies they got 'around' 3.3% 4-ho-whatever out, adding tryptamine or DET, thats pretty good.. if its near 100% efficient, i'd guess over 90%.

Normally without adding DMT or tryptamine etc the number (psilocin/cybin combined) its closer to 0.5% (maybe less?) for typical mushrooms. Well my friends friend around the world somewhere plans to try this *sometime* soon, it would be nice to get some numbers figured out - example being just adding ground up mimosa bark, how many grams of bark for x grams substrate? etc. Sort of like a super shroom TEK I guess.

A previous msg from someone who's experimented with adding various amounts of DMT to substrate to 'pf style' jars, found that as long as it was at least 50mg, it'd be fully saturated and adding more won't get you even more potent shrooms - but it wouldn't hurt if there was extra DMT either.

There's at least 50mg's of DMT in only ~6g mimosa bark..

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Oh yeah, I had a thought about how the couple times I had these REALLY potent cyanescens, I noticed the trip kicked in super fast, recently found this,

http://www.tacethno.com/info/psilocybe/gartz1.txt

It looks like for the most part cyanescens produce a fuckload more psilocin, and cubensis its mostly psilocybin, so a mostly psilocin filled cubensis mushroom grown from some mimosa bark (most of the potentiation will be from 4-ho-dmt) might produce a trip thats just like eating some real super potent cyanescens.

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Okay i am not a big writer and am writing this a year after growing and a week after tripping but i will try to give you some information witch might or might not interest the myco- or tripto-files reading this.
The idea behind this experiment came after reading the statement of A. Shulgin in his book Tihkal under 4-oh-det that maybe the mushroom was a "x in, 4-oh-x out"-machine, and the papers by J.Garz.
I had grown some cubensis using rye. I took one of my jars of completely collonated rye and added 100mg dipt to a small amount of soil and vermucellite. I had done this because in the earlyer stage of rye colonization i would normally loose some jars to infection. After a certain amount of time cubensis mushrooms started to appear. Unluckily i could harvest just a small amount of mushrooms before the rest of the culture got infected. It is with these mushrooms i did my experiment. I had given some to a friend who had reported eating just a little amount. But since the effects did not develop within 15 minutes (and he had taken a small dose) he concluded that he had not had an 4-oh-dipt effect.

Setting: a cottage in sweden
Substances: half a B-100 pill, half a C-1000 pill, about 2mg of deprenyl (held under the tung), half a 40mg inderal pill, four OO capsules of mushroom powder with about 3,1 grams of mushroom powder total, half a 50mg tofisopam tablet.

Chronology:

11:00 or 12:00 : had eaten breakfast of pasta from the dinner the day before

13:50 : Decide to take the mushrooms today. And take some b-vit, c-vit (both time release), inderal, and deprenyl. I take the first two almost every day. The deprenyl i take two or three times per week. I should probably have left the deprenyl out. But after an earlyer experience where i had purposefully abstained from deprenyl for 10 days, so as to not mess with my first taste of Methylone, and then found the effect of Methylone (180mg) to be almost completely absent i decided that i should make a point of taking my "medicine"that day (someone else taking 1 mg every day had had no problems with Methylone, and i had read a comment of one guy who had much less effect from mdma when taking 5mg deprenyl three days prior, but not when taking directly prior to mdma). Of course Methylone and mdma are no triptamines, but hey, i did what i did anyway. The inderal was to combat the panic wich sometimes comes over me when taking a psychedelic these days. I did not want the panic to spiral out of control, and thought of it as a possibility to overcome that panic just as stage fright can be overcome by taking inderal once or a few times before an event, and then by positive learning making the use of the substance unneccesary. The inderal impacted my trip but i think it also did do what i intended.

14:00 : I drop the first of four capsules. pack a bag with some provisions and cd's. Put on "bitches brew" by Miles Davis and leave the cottage to have a walk. Short panic, what have i done? Is this what i want? But i go my way and think no more of it. After about ten or more minutes of walking i have an alert that something is happening, but it does not develop.

14:15 : I took another capsule of mushroom powder. Since 4-oh-dipt seems to be a fast and hard hitter i had planned to space the four capsules 15 minutes apart. If the mushrooms tuned out to be more potent than normal mushrooms (wich would mean there would at least be some 4-oh-dipt along with psilocybin, i had read of up to 3% strength with triptamine/DET fed mushrooms) i would have some time to find out before taking a full dose. The swedish scenery was beautiful with little fields with cows forrest, and a little stream running threw. I decide to lay down on a natural stone wall and enjoy the view. No more than the alert before.

14:35 : Take another capsule. I am surprised not to feel any more than i do. Normally i would feel something within half an hour of taking mushrooms. Maybe a year of storage might have decreased potency quite a bit.

14:40 : What the fuck, i drop the last capsule and head back to the cabin. The scenery makes me think this would be a good place to take acid. Probably because it reminds me of switzerland where i had taken my first day time acid.

Ok now the chronology gets lost. I can just estimate.
When coming back to the cabin (probably about ten or fifteen minutes after taking the last capsule) i sit down in the garden and look at the lake and the trees surrounding me. I start to feel the trip. It feels different than a mushroom trip. I come to the conclusion that this is : more purple than mushrooms... and think the description is quite funny. Hi hi..
When looking at my surroundings the purpleness reminds me of 2-ct-7 (probably because the package(blue mystic) was blue/purple) and also reminds me of it and other substances because it feels "chemical" (not dirty just not organic). I do not feel a connection with the trees, nature seems uninteresting ( in contrast to mushrooms, lsd, or san pedro). I decide to close my eyes and check it out. A swirling..*snip*

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I have a side thought as well : Isnt psilocin less stable than psilocybin? I mean if one aims at long term storage of his fruits maybe a huge amount of psilocin and not too much psilocybin wouldnt be a good idea!

Unless of course one could find a way of forcing mycelium to turn psilocin to psilocybin!

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shirley knott said:

this thread is five years old, even the last post is three months ago. c'mon dude, please!

'Advanced Mycology' forum is chock full of threads (approx one in every five, i reckon) concerning adding tryptamines to substrate to increase mushroom potency. every time it's someone who thinks this is a groundbreaking new concept, but has no alternative forum to post this in. since they're usually fairly new themselves, they don't search or even scan the first page for ideas. it'd be a major enhancement to this forum if someone who knows this topic thoroughly could collect and compile a nice stock answer, preferably with the occasional pic to break up the text, that recognises all the major points.



then at least these threads could all be closed and referred to one source. it'll still mean there's at least two threads on the first page dealing with this subject, but at least it won't be eight threads.


anyone wanna take this on? 

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There is a very interesting study that took place in Leipzig about 15 years ago. Jochen Gartz, a mushroom explorer whom I know quite well, has done some fascinating studies with Psilocybe species by raising them on solid media containing strange tryptamines that are alien to the mushroom. Apparently the enzymes that are responsible for the 4-hydroxy group of psilocin are indifferent to what it is they choose to 4-hydroxylate. He has taken things like DPT or DIPT and put them in the growth media and the fruiting bodies that came out contain 4-hydroxy-DPT or 4-hydroxy-DIPT instead of psilocin. In fact, he has a patent on the process. These active compounds are made by the mushroom so they really are natural and yet they never have been observed in nature. I'll give you even odds that if you put spores of a psilocybe species on cow droppings loaded with 5-MeO-DMT you would come out with mushrooms containing 4,5-HO-MeO-DMT. This way you avoid a 10 step synthesis by growing a psychoactive mushroom that contains no illegal drug.

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shirley knott said:

'Advanced Mycology' forum is chock full of threads (approx one in every five, i reckon) concerning adding tryptamines to substrate to increase mushroom potency. it'd be a major enhancement to this forum if someone who knows this topic thoroughly could collect and compile a nice stock answer, preferably with the occasional pic to break up the text, that recognises all the major points.

anyone wanna take this on? 

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