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InvisibleGnuBobo
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Alcohol to kill living mold and spores?
    #4100492 - 04/26/05 10:33 PM (11 years, 7 months ago)

I'm fairly sure that alcohol kills live mold but as for spores...I don't know. I tried searching the archives and I didn't find a satisfactory answer. I know that bleach will kill spores and organisms, but I don't know the range of potential contaminants that alcohol will sterilize/kill on contact.
Anyone have an answer for this?
Thanks.


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InvisibleHolydiver
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Re: Alcohol to kill living mold and spores? [Re: GnuBobo]
    #4100512 - 04/26/05 10:38 PM (11 years, 7 months ago)

Yep, rubbing alcohol (isopropyl) will kill all bacteria and spores. That and ethyl alcohol are equivalent to bleach for killing power, just more expensive.


--------------------
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InvisibleGnuBobo
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Re: Alcohol to kill living mold and spores? [Re: Holydiver]
    #4100600 - 04/26/05 11:04 PM (11 years, 7 months ago)

Thanks!


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Offline_JJ_
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Re: Alcohol to kill living mold and spores? [Re: GnuBobo]
    #4101737 - 04/27/05 05:09 AM (11 years, 7 months ago)

Quoting this:

Chlorine
? Chlorine kills bacteria, fungi, viruses and endospores.
? It is an oxidising agent and works by denaturing proteins.
? It is used in the treatment of drinking water.
? Chlorine-based disinfectants are used in the home and laboratory for
disinfecting surfaces.

Alcohol
? Alcohol kills bacteria, fungi and some viruses (if left long enough).
? It works by dissolving cell membranes.
? It is used in the laboratory for swabbing benches and disinfecting
instruments such as scalpels.

So perhaps alcohol does not, in fact, kill spores?


Edited by _JJ_ (04/27/05 05:12 AM)


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OfflineFleaAccela
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Re: Alcohol to kill living mold and spores? [Re: _JJ_]
    #4101761 - 04/27/05 05:38 AM (11 years, 7 months ago)

chapter 18 - g, sub section i

"Like disinfectants in general, alcohols are ineffective against endospores or many viruses"

http://www.mansfield.ohio-state.edu/~sabedon/biol2032.htm


--------------------
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Invisibleagar
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Re: Alcohol to kill living mold and spores? [Re: GnuBobo]
    #4101768 - 04/27/05 05:43 AM (11 years, 7 months ago)

INFO FROM HERE............!

Name: ALCOHOLS
Ethyl alcohol (ethanol) and isopropyl alcohol?s are the most frequently used alcohol?s.
Formula:
Ethanol: CH3CH2?OH Isopropanol: CH3?CH?OH

Inflammable. CH3
Effect:
Alcohol?s kill vegetative forms of bacteria (including TB) and fungi,but have no action on spores or viruses (there might, however, be some effect on kappeb?rende vira).Their effect depends on concentration and type of alcohol.

The following three solutions have similar effect:
Ethanol 70% , isopropanol 60% and n-propanol 40%
70-80% alcohol inactivates HIV and Hepatitis B in 2-10 minutes.

How does it work:
Alcohol?s precipitate proteins and solubilize lipids present in cell membranes.
It has a rapid action.
Contact time should preferably be 10-30 minutes.
Toxicity:
Ethanol is absorbent and astringent and dries out the skin. Propanoles are less absorbent, but they might penetrate the skin. The brief irritant action seen on the skin , when alcohol is applied in high concentrations, results from partial precipitation of cellular proteins and from its dehydrating actions. If strong alcohol vapors are inhaled, the irritation causes reflex closure of glottis.

Effect on other materials:
Non-corrosive
May damage rubber, shellac and plastic
Interaction:
Synergistic effect together with iodine, chlorhexidin and quaternary ammonium compounds.
Alcohol?s are easily inactivated by organic material.
Use:
Hands, skin and instruments and surfaces: 60-70% ethanol.
In this case the effect is not enhanced with higher concentration, as it takes water to practice its effect


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Offlineclosetgrower
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Re: Alcohol to kill living mold and spores? [Re: agar] * 1
    #4102039 - 04/27/05 10:45 AM (11 years, 7 months ago)

:handth:


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OfflineMycroProphecy
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Re: Alcohol to kill living mold and spores? [Re: agar]
    #16553715 - 07/19/12 11:42 AM (4 years, 4 months ago)

Quote:

agar said:
INFO FROM  HERE............!

Name: ALCOHOLS
Ethyl alcohol (ethanol) and isopropyl alcohol?s are the most frequently used alcohol?s.
Formula:
Ethanol: CH3CH2?OH Isopropanol: CH3?CH?OH

Inflammable. CH3
Effect:
Alcohol?s kill vegetative forms of bacteria (including TB) and fungi,but have no action on spores or viruses (there might, however, be some effect on kappeb?rende vira).Their effect depends on concentration and type of alcohol.

The following three solutions have similar effect:
Ethanol 70% , isopropanol 60% and n-propanol 40%
70-80% alcohol inactivates HIV and Hepatitis B in 2-10 minutes.

How does it work:
Alcohol?s precipitate proteins and solubilize lipids present in cell membranes.
It has a rapid action.
Contact time should preferably be 10-30 minutes.
Toxicity:
Ethanol is absorbent and astringent and dries out the skin. Propanoles are less absorbent, but they might penetrate the skin. The brief irritant action seen on the skin , when alcohol is applied in high concentrations, results from partial precipitation of cellular proteins and from its dehydrating actions. If strong alcohol vapors are inhaled, the irritation causes reflex closure of glottis.

Effect on other materials:
Non-corrosive
May damage rubber, shellac and plastic
Interaction:
Synergistic effect together with iodine, chlorhexidin and quaternary ammonium compounds.
Alcohol?s are easily inactivated by organic material.
Use:
Hands, skin and instruments and surfaces: 60-70% ethanol.
In this case the effect is not enhanced with higher concentration, as it takes water to practice its effect





Very informative. Thank ya.


--------------------

Yummy Shroom Tea Tek for 4

"I have found some kind of temporary sanity in this." MJK


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InvisibleDoc_T
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Re: Alcohol to kill living mold and spores? [Re: MycroProphecy]
    #16554057 - 07/19/12 01:12 PM (4 years, 4 months ago)

In teh seven years since this thread was posted, it's become widely known that alcohol does not kill mold spores.


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OfflineTheInternetPolice
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Re: Alcohol to kill living mold and spores? [Re: GnuBobo]
    #21286419 - 02/17/15 06:23 AM (1 year, 9 months ago)

old thread, i know.. but i feel that people ignore the potential of acetone, acetone is usually not denatured, it has high evap pressure relative to ethanol and lower BP, meaning it should be  very effective for killing bacteria, as ethanol can do if we suppose acetone wouldnt mess up the spores
if acetone is filled into a small spraybottle it should be able to kill bacteria and leave totally disinfected spores behind in less than a minute, perhaps less than 20 seconds of the paper or whatever the spores are placed on is slightly heated up

question is whether acetone does or does not kill spores, as it is much less polar and thus a stronger solvent and may be able to break down the spores by dissolving them, where ethanol cannot


--------------------
"All legislation whatsoever is an absurdity, a usurpation, and a crime."
-Lysander Spooner
so far: Morphine, LSD, Ketamine, 2C-B, LSA, THC, MDMA, mushrooms, salvia, caffeine, nicotine and alcohol
to do: DMT, meth, mescaline, n-bome, MDA pure, N2O, kratom, brugmasia, Opium and heroic cannabis dose.


shroomtip: stopper nose with cotton wool, no taste.


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Invisibleblindingleaf
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Re: Alcohol to kill living mold and spores? [Re: TheInternetPolice]
    #21286476 - 02/17/15 07:15 AM (1 year, 9 months ago)

im gonna stick with good sterile technique and iso @ 70% :shrug:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinevan der griegen
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Re: Alcohol to kill living mold and spores? [Re: blindingleaf]
    #21286482 - 02/17/15 07:20 AM (1 year, 9 months ago)

Acetone will fog your SAB permanently, and the reason it isn't denatured is because who the fuck would drink acetone?  And what does denaturing have to do with killing spores?


--------------------
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Oat Prep Tek
Bored?  Please take one of my experiments off my hands.
THE NOOB FORUM


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Invisibleblindingleaf
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Re: Alcohol to kill living mold and spores? [Re: van der griegen]
    #21286489 - 02/17/15 07:27 AM (1 year, 9 months ago)

Quote:

because who the fuck would drink acetone?




when u serve me a drink, please stick it in the fridge, cause i like cold beverage


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleInocuole
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Re: Alcohol to kill living mold and spores? [Re: TheInternetPolice] * 1
    #21286505 - 02/17/15 07:38 AM (1 year, 9 months ago)

Quote:

TheInternetPolice said:
old thread, i know.. but i feel that people ignore the potential of acetone, acetone is usually not denatured, it has high evap pressure relative to ethanol and lower BP, meaning it should be  very effective for killing bacteria, as ethanol can do if we suppose acetone wouldnt mess up the spores
if acetone is filled into a small spraybottle it should be able to kill bacteria and leave totally disinfected spores behind in less than a minute, perhaps less than 20 seconds of the paper or whatever the spores are placed on is slightly heated up

question is whether acetone does or does not kill spores, as it is much less polar and thus a stronger solvent and may be able to break down the spores by dissolving them, where ethanol cannot




I just wanted to drop in and say fuck you for bumping this thread after acknowledging how old it was, and then not having any good info in your bump. 
:shrug:


--------------------
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InvisibleHashish
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Re: Alcohol to kill living mold and spores? [Re: Inocuole]
    #21287412 - 02/17/15 01:07 PM (1 year, 9 months ago)

:whathesaid:


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Offlineconcrete_666
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Re: Alcohol to kill living mold and spores? [Re: Hashish]
    #21287610 - 02/17/15 01:51 PM (1 year, 9 months ago)

What are your opinions about using everclear...or any 190 proof?


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Which came first, the chicken or the egg? If heaven was perfect, how was there a fallen Angel, before sin?


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OfflineTheInternetPolice
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Re: Alcohol to kill living mold and spores? [Re: Hashish]
    #21287670 - 02/17/15 02:02 PM (1 year, 9 months ago)

well in europoor, at least my region.. isopropyl alcohol is very hard if not impossible to come by for whatever reason, and as you add denatonium benzoate to your growing you will about entirely surely get it into your mouth, as if the shrooms doesnt already taste bad enough

as for "Acetone will fog your SAB permanently" i guess it stands for Still Air Box? the idea is to evap the acetone out, this wouldnt require vacuum or dragging the air out of whatever container is used to evap the spores to dryness as acetone has a great evaporative pressure when heated just slightly
t*C    mmHg vapour pressure
0.56     71.8307
11.32   123.293
22.08   202.111
32.84   318.207
43.6     483.462
54.36   711.703
65.12   1018.63

i accept the fuck you, tho.. i hope you will regain your loss asap


--------------------
"All legislation whatsoever is an absurdity, a usurpation, and a crime."
-Lysander Spooner
so far: Morphine, LSD, Ketamine, 2C-B, LSA, THC, MDMA, mushrooms, salvia, caffeine, nicotine and alcohol
to do: DMT, meth, mescaline, n-bome, MDA pure, N2O, kratom, brugmasia, Opium and heroic cannabis dose.


shroomtip: stopper nose with cotton wool, no taste.


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InvisibleHashish
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Re: Alcohol to kill living mold and spores? [Re: concrete_666]
    #21287672 - 02/17/15 02:03 PM (1 year, 9 months ago)

i was actually gonna ask about the "heads" or "tails" of a shine run. i know you can clean your house with heads lol. but will it kill mold spores? if i can recycle this way it would save me some money.


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Offlinevan der griegen
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Re: Alcohol to kill living mold and spores? [Re: TheInternetPolice]
    #21289353 - 02/17/15 06:12 PM (1 year, 9 months ago)

Quote:

TheInternetPolice said:
well in europoor, at least my region.. isopropyl alcohol is very hard if not impossible to come by for whatever reason, and as you add denatonium benzoate to your growing you will about entirely surely get it into your mouth, as if the shrooms doesnt already taste bad enough





In North America, ethanol comes in big, cheap cans denatured with methanol.  But benzoates aren't unheard of, and I do know what you're talking about.  But there are very few real uses for sanitizers like that, and the only one I ever use it for is hosing down the inside of my still air box.  I don't dunk my mushrooms in it, or add it to my substrate.

Quote:

TheInternetPolice said:
as for "Acetone will fog your SAB permanently" i guess it stands for Still Air Box? the idea is to evap the acetone out, this wouldnt require vacuum or dragging the air out of whatever container is used to evap the spores to dryness as acetone has a great evaporative pressure when heated just slightly





Yes, it evaporates quite nicely.  It also rips the plasticizers and smaller plastic molecules out of the walls of any plastic vessel, rending it translucent but far from transparent.  You can't see shit through it anymore, and it has the same milky color that a bottle of fingernail polish remover has.  In fact, nail polish remover is made of acetone, and you'll never, ever see it in a clear plastic bottle for this reason.

Feel free to experiment, though.  I was just trying to save you a few bucks and a few hours of work.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.
THE NOOB FORUM


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OfflineJudPie
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Re: Alcohol to kill living mold and spores? [Re: van der griegen]
    #21289478 - 02/17/15 06:45 PM (1 year, 9 months ago)

Quote:

van der griegen said:

Yes, it evaporates quite nicely.  It also rips the plasticizers and smaller plastic molecules out of the walls of any plastic vessel, rending it translucent but far from transparent.  You can't see shit through it anymore, and it has the same milky color that a bottle of fingernail polish remover has.  In fact, nail polish remover is made of acetone, and you'll never, ever see it in a clear plastic bottle for this reason.

Feel free to experiment, though.  I was just trying to save you a few bucks and a few hours of work.




:whathesaid:

I may not be a user with a trillion posts, or an award winning chemist, but 5 mins on Google will show you this readily....


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