Twigg said:

Ive had a plate in the back of one of the drawers in my fridge for the past 5-6 years ive been on a hiatus.
what's the chance the mycelium is still alive?

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Tri said:

Mystikal said:

Belome said:

looking to sterilize my petri dishes soon. is there an established tek out there for this? i've never done it before. unclear on how to properly store them after sterilizing and for how long i can expect them to stay sterile until i'm ready to pour agar.

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Wait... I need to sterilize new petri dishes?

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No, most all Petri dishes come sterile in packaging

@Belome I wrap mine in tinfoil, they also make very handy little tea canisters that fit plates well or the actual lab equipment meant to house Petri dishes in the autoclave, of course

Edit: I wrap in foil and then pressure cook at 15 psi for 25-30 minutes, to be clear

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Like this






These plastic pickle containers work good for my holy grails.  Dont know if glass would fit  But maby.    Use foil for non pp5 lids 



Incase your interested in using some sorta container.

Huskies said:

I think you would still have a blob prone culture on you, and the better plan would be to go back to spores to remove this genetic imprint.



I tried cleaning up this culture by cloning fruits, but alas, the blob clusters remained.

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koma23 said:

Huskies said:

I think you would still have a blob prone culture on you, and the better plan would be to go back to spores to remove this genetic imprint.



I tried cleaning up this culture by cloning fruits, but alas, the blob clusters remained.

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Ugh, disgusting : D How many generations did you try?

I don't have spores unfortunately ... No idea if Haole even drops them. Maybe a swab would do, idk.

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Huskies said:

Only once or twice. I remember I had this perfect squat fruit, 50g+, cloned it, blobs galore

Most albinos don't seem to sporulate much, so swabs would be the way to go.

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Ah, once or twice might not have been enough ... I'll try a heavy top layer p9 style and/or just casing it.

Anybody else ever tried such an endeavour?

callmehippie said:

koma23 said:

Huskies said:

I think you would still have a blob prone culture on you, and the better plan would be to go back to spores to remove this genetic imprint.



I tried cleaning up this culture by cloning fruits, but alas, the blob clusters remained.

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Ugh, disgusting : D How many generations did you try?

I don't have spores unfortunately ... No idea if Haole even drops them. Maybe a swab would do, idk.

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Did every flush consistently throw blobs? Or did you have any of that second flush Magic?

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Mashedpotatoes said:

are 24x24mm glass slips too small for cabin sequestering? i thought it'd be larger than it was when i got it today

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It's plenty big, an inch. I have those. But I sterilize them in a glass petri 10 or so at a time and usually can't be bothered to fish one out cleanly so just do agar roofs. I think Kloakk cuts thicker actual slides in half and individually wraps in foil and sterilizes- a great method (also with the cover slips). I'm comfortable working with the thin cover slips but I ain't got time to individually wrap.

DV8DUG said:

I seriously doubt you can remove blobbing genetics by selection alone. I believe it is an environmental condition that occurs, just more noticeable in PE strains. It would be like trying to have a litter of dogs that didn't bark or chase cars or whatever.

When in doubt, just recase and go along like normal.

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