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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Marxcelium] * 4
    #29724072 -

Oven safe dish, squares of foil cut the way I want, a large piece of foil over that. Bake at 450 F for 30-45 min. Let cool and open in SAB or FH


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:headbang3: Pastywhyte's Easy Writeups :headbang3: My Favorite Grow Logs :headbang3:
:headbang3: Avoiding the dreaded facepalm in cultivation :headbang3: The worst threads all have the same subjects :headbang3:
:headbang3: Q & A.M.U. :headbang3:

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Re: Ask Quick Questions, Get Quick Answers [Re: Pastywhyte] * 2
    #29724127 -

Ive had a plate in the back of one of the drawers in my fridge for the past 5-6 years ive been on a hiatus.
what's the chance the mycelium is still alive?


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Yahoo deleted my email from inactivity that I use to use to access my old account from back in 2018.
-Until I can get that sorted out im back on a new account. (Yuck)

:migraine:

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Re: Ask Quick Questions, Get Quick Answers [Re: Twigg] * 3
    #29724133 -

Only one way to find out.


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"it's all a joke between mom contractions and coffin fittings"


The most useful tool for noobs

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Re: Ask Quick Questions, Get Quick Answers [Re: Tight Lunchbox] * 2
    #29724148 -

:whathesaid: even dry plates can come alive


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Twigg]
    #29724176 -

Twigg said:
Ive had a plate in the back of one of the drawers in my fridge for the past 5-6 years ive been on a hiatus.
what's the chance the mycelium is still alive?


Dude, do some transfers!! I'm 100% positive everyone here would love to see the results, especially if it started growing again.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
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Things that saved Lo-Z
PANS IN TUBS :mushroom2:
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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #29724201 -

Tri said:
Mystikal said:
Belome said:
looking to sterilize my petri dishes soon. is there an established tek out there for this? i've never done it before. unclear on how to properly store them after sterilizing and for how long i can expect them to stay sterile until i'm ready to pour agar.


Wait... I need to sterilize new petri dishes?


No, most all Petri dishes come sterile in packaging

@Belome I wrap mine in tinfoil, they also make very handy little tea canisters that fit plates well or the actual lab equipment meant to house Petri dishes in the autoclave, of course

Edit: I wrap in foil and then pressure cook at 15 psi for 25-30 minutes, to be clear



Like this






These plastic pickle containers work good for my holy grails.  Dont know if glass would fit  But maby.    Use foil for non pp5 lids 



Incase your interested in using some sorta container.

Edited by callmehippie (11/11/26 12:28 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29724267 -



Jack Frost

I inoculate exactly 18 Day's ago LC 4cc


How it looks and how long it will take too full Cologinze the Jar?

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet]
    #29724323 -

are 24x24mm glass slips too small for cabin sequestering? i thought it'd be larger than it was when i got it today :sunny:


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I wish you bountiful fruits and godspeed :heart:

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Re: Ask Quick Questions, Get Quick Answers [Re: Mashedpotatoes]
    #29724329 -

I'm messing with a new Haole culture, this is my first grow with it. Only a thin top layer was applied at spawn. It blobbed out.



If I was to continually clone non blon fruits of the first flush, would I eventually have a culture that doesn't blob and doesn't need a casing?



Is that a realistic goal or should I just accept the culture as it is and case?

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Re: Ask Quick Questions, Get Quick Answers [Re: koma23]
    #29724368 -

I think you would still have a blob prone culture on you, and the better plan would be to go back to spores to remove this genetic imprint.



I tried cleaning up this culture by cloning fruits, but alas, the blob clusters remained.


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I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29724375 -

Huskies said:
I think you would still have a blob prone culture on you, and the better plan would be to go back to spores to remove this genetic imprint.



I tried cleaning up this culture by cloning fruits, but alas, the blob clusters remained.


Ugh, disgusting : D How many generations did you try?

I don't have spores unfortunately ... No idea if Haole even drops them. Maybe a swab would do, idk.

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Re: Ask Quick Questions, Get Quick Answers [Re: koma23] * 2
    #29724378 -

Only once or twice. I remember I had this perfect squat fruit, 50g+, cloned it, blobs galore

Most albinos don't seem to sporulate much, so swabs would be the way to go.

Crucial advice: swab very gently, and don't use it immediately on agar, give it a few days, if not even a week or two. This is to not accidentally clone microscopic gill pieces, which would just be another clone.

Giving the swab a week to dry out makes it less likely since those cells would die off, hopefully. If you start getting organized mycelium on day on on agar, that might also be a sign that it is an accidental clone.

Easier to do with varieties that actually produce dark spores, easier to see. With albinos where you mostly see bruising on the swab, harder to say.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Lo-Z] * 1
    #29724387 -

It looks a little dirty around the edges like dried agar but the actual agar and mycelium look okay.

I believe this is a pan Jamaica.
I never got a successful pan grow as this was far as i got with them before i took my hiatus after multiple failed tubs/spawn and dishes of regular cubes.

I was doing fine for years then boom every grow got trich or some black mold so I put this in the fridge and threw away most of my supplies.



--------------------
Yahoo deleted my email from inactivity that I use to use to access my old account from back in 2018.
-Until I can get that sorted out im back on a new account. (Yuck)

:migraine:

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Re: Ask Quick Questions, Get Quick Answers [Re: koma23]
    #29724489 -

koma23 said:
Huskies said:
I think you would still have a blob prone culture on you, and the better plan would be to go back to spores to remove this genetic imprint.



I tried cleaning up this culture by cloning fruits, but alas, the blob clusters remained.


Ugh, disgusting : D How many generations did you try?

I don't have spores unfortunately ... No idea if Haole even drops them. Maybe a swab would do, idk.



Did every flush consistently throw blobs? Or did you have any of that second flush Magic?


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies]
    #29724493 -

Huskies said:
Only once or twice. I remember I had this perfect squat fruit, 50g+, cloned it, blobs galore

Most albinos don't seem to sporulate much, so swabs would be the way to go.




Ah, once or twice might not have been enough ... I'll try a heavy top layer p9 style and/or just casing it.

Anybody else ever tried such an endeavour? :smile:

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29724538 -

callmehippie said:
koma23 said:
Huskies said:
I think you would still have a blob prone culture on you, and the better plan would be to go back to spores to remove this genetic imprint.



I tried cleaning up this culture by cloning fruits, but alas, the blob clusters remained.


Ugh, disgusting : D How many generations did you try?

I don't have spores unfortunately ... No idea if Haole even drops them. Maybe a swab would do, idk.



Did every flush consistently throw blobs? Or did you have any of that second flush Magic?


I did get flushes with more fruits, but overall, the blobs were always present.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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