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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Doozberry]
    #29723929 -

I doubt it's you're tub causing this, I think it's worth trying to clean those plates up using an Island, a Cabin. or Water agar

Degrees of contamination certainly vary between cultures. Most the time they can be separated with the aformentioned teks, or . Though once in awhile there's one thats a consistent problem, I'm not saying yours is like mine was, I'm just speaking from experience on a particularly dirty culture I had that lined up with the appearance of your situation.

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Re: Ask Quick Questions, Get Quick Answers [Re: MushLuvR77]
    #29723948 -

MushLuvR77 said:
Clean spawn will colonize very quickly, roughly 5-7 days, the cloudy patchy growth is usually early contamination, the clarity of the surface isn't great in your pic, so it's tough to give a definitive mold, but I can tell you from my experience, it's to the compost heap, it's DONE!


Thank you much. Tubs will go into the flower garden tomorrow, away from the growing area.

It's been really cold here, in the 50-60sยฐF, and the other tubs that do not show the white mold are not colonizing to my eye either.

Thanks again for your expertise.

Mike


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Mycelium? It's not MYcelium! It's YOURcelium!

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Re: Ask Quick Questions, Get Quick Answers [Re: MushLuvR77]
    #29723954 -

Awesome man much appreciated n how much water do u add to get it to field capacity


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New Journal and Grow setup
https://www.shroomery.org/forums/showflat.php/Number/29668030

Cloud Nyne isnt a destination its the frequency i keep

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Re: Ask Quick Questions, Get Quick Answers [Re: CloudNyne420]
    #29724015 -

Anyone have advice for starting conversations you don't wanna have?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 2
    #29724016 -

Just get drunk and speak your mind.
:batlol:

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29724017 -

i like by telling people that im about to have a conversation i dont want to have.


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29724019 -

tryptkaloids said:
Anyone have advice for sta rting conversations you don't wanna have?



What do you mean?  conversations that involve confronting somebody in some way?

Or like being honest with somebody in a way that might hurt their feelings?

or telling them what they need to hear instead of what they want to hear type of thing?


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:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy]
    #29724020 -

It's a conversation that will help in the long run and it's not necessarily that I don't wanna have it, more that Its one I've never had to have and I I'm nervous I wont communicate my wants and needs well for some reason


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #29724025 -

this might be the wrong thread my man :lol:


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: Ask Quick Questions, Get Quick Answers [Re: mushboy] * 1
    #29724032 -

looking to sterilize my petri dishes soon. is there an established tek out there for this? i've never done it before. unclear on how to properly store them after sterilizing and for how long i can expect them to stay sterile until i'm ready to pour agar.


--------------------

Ack! It's a snack attack!
And YOU'RE it!

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Re: Ask Quick Questions, Get Quick Answers [Re: Belome]
    #29724035 -

Belome said:
looking to sterilize my petri dishes soon. is there an established tek out there for this? i've never done it before. unclear on how to properly store them after sterilizing and for how long i can expect them to stay sterile until i'm ready to pour agar.


Wait... I need to sterilize new petri dishes?

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Re: Ask Quick Questions, Get Quick Answers [Re: Mystikal] * 1
    #29724037 -

Only sterilize petris labeled reusable. Most come sterile.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Belome]
    #29724038 -

these are glass and didn't come in any kind of sleeve that would suggest to me that they are sterile. i thought better to be safe than sorry?

edit: let's pretend i got my petri dishes, laid them out on the counter and sneezed on all of them


--------------------

Ack! It's a snack attack!
And YOU'RE it!

Edited by Belome (11/10/26 08:10 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Mystikal] * 1
    #29724040 -

Mystikal said:
Belome said:
looking to sterilize my petri dishes soon. is there an established tek out there for this? i've never done it before. unclear on how to properly store them after sterilizing and for how long i can expect them to stay sterile until i'm ready to pour agar.


Wait... I need to sterilize new petri dishes?


No, most all Petri dishes come sterile in packaging

@Belome I wrap mine in tinfoil, they also make very handy little tea canisters that fit plates well or the actual lab equipment meant to house Petri dishes in the autoclave, of course

Edit: I wrap in foil and then pressure cook at 15 psi for 25-30 minutes, to be clear


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๐•ด๐–‹ ๐•ด ๐–๐–†๐–›๐–Š ๐–˜๐–Š๐–Š๐–“ ๐–‹๐–š๐–—๐–™๐–๐–Š๐–— ๐–Ž๐–™ ๐–Ž๐–˜ ๐–‡๐–ž ๐–˜๐–™๐–†๐–“๐–‰๐–Ž๐–“๐–Œ ๐–š๐–•๐–”๐–“ ๐–™๐–๐–Š ๐–˜๐–๐–”๐–š๐–‘๐–‰๐–Š๐–—๐–˜ ๐–”๐–‹ ๐•ฒ๐–Ž๐–†๐–“๐–™๐–˜

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Belome]
    #29724042 -

If they are sterilized from the manufacture, there is no need to sterilize them. They will stay sterile for as long as they stay shut, and for as long as you maintain good sterile technique.

If they are used glass plates and you want to re-sterilize, just run them in the PC put together bottoms and top, and then don't open them until you're ready to pour the new plates. a quick 30-45minutes in the PC at 15psi should be fine.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 2
    #29724044 -

tryptkaloids said:
Only sterilize petris labeled reusable. Most come sterile.


Thanks for that info..I might do my first ever agar work later on.

Trying to clone this albino treasure coast I had in my first flush.


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Re: Ask Quick Questions, Get Quick Answers [Re: Tri]
    #29724045 -

Glass should be sterile at similar times to liquids ie minimal


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Belome] * 1
    #29724048 -

For the glass ones I wrap them in foil I. 5 stacks and place them on top of a wide mouth half pint jar with some water in them so they don't float. then vent for 10 min and cook for 30min.

Can unwrap and store them in the SAB then:mushroom2:

Hey I saw you're pms Belome, responding shortly!:hug:

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Re: Ask Quick Questions, Get Quick Answers [Re: Lo-Z]
    #29724049 -

Lo-Z said:
If they are sterilized from the manufacture, there is no need to sterilize them. They will stay sterile for as long as they stay shut, and for as long as you maintain good sterile technique.

If they are used glass plates and you want to re-sterilize, just run them in the PC put together bottoms and top, and then don't open them until you're ready to pour the new plates. a quick 30-45minutes in the PC at 15psi should be fine.


yeah i definitely took some of them out of the box and checked them out assuming i'd just be sterilizing them later. this is my first time handling petri dishes at all, was never much of a science person.

thanks for the info


--------------------

Ack! It's a snack attack!
And YOU'RE it!

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Re: Ask Quick Questions, Get Quick Answers [Re: Belome]
    #29724067 -

Iโ€™ve never done glass so canโ€™t speak to it first hand, but I always thought LotKidโ€™s system seemed the most elegant with the metal canisters in the oven.

https://www.shroomery.org/forums/showflat.php/Number/27674569#27674569

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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