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North Spore Shop: Injection Grain Bag

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Glass Petri Dishes sterilisation
    #27674239 -

I’ve been sterilising my glass Petri dishes in the oven… im assuming it’s possible to do so in a PC too.

I was thinking of wrapping them in foil, then PCing them for about an hour 15psi

Doable?


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Re: Glass Petri Dishes sterilisation [Re: Zombie-ant]
    #27674289 -

Zombie-ant said:
I’ve been sterilising my glass Petri dishes in the oven… im assuming it’s possible to do so in a PC too.

I was thinking of wrapping them in foil, then PCing them for about an hour 15psi

Doable?


Absolutely doable.  That's roughly how I do mine but I don't wrap them in foil.  I have a perforated stainless steel container that I stack them in. I added a handle to it and cut a slit down the side so I can lift them out and place lengths of nylon string between the dishes to keep the water from sticking them together.

And to be honest, I've been considering switching to sterilizing them in the over. PCing them comes with its own set of issues. Condensation, glass sticking together...  Individually wrapping them in foil would solve one of those problems for sure.  Then of course you have to individually wrap each dish.

An hour at 15psi is overkill. 30 minutes at 15psi is sufficient as long as all your PCing is the petri dishes.


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Agar and Liquid Culture Formulas


Equipment, Teks, Etc
FF Lid Tek

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Re: Glass Petri Dishes sterilisation [Re: father_fungi]
    #27674312 -

Father Fungi how much water spotting do your plates get?


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:sporedrop: GLOSSARY  :sporedrop: ACROMYMS!   :sporedrop: GETTING STARTED :sporedrop:

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Re: Glass Petri Dishes sterilisation [Re: Zombie-ant]
    #27674380 -

Zombie-ant said:
I’ve been sterilising my glass Petri dishes in the oven… im assuming it’s possible to do so in a PC too.

I was thinking of wrapping them in foil, then PCing them for about an hour 15psi

Doable?


that's how I sterilize my PP5 petri dishes.

However, there are always condensation in the dish, although they don't cause contamination issues, it's harder to pour a glassy plate with left over water in the dish.


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Re: Glass Petri Dishes sterilisation [Re: MycoWeek]
    #27674423 -

The last glass petri batch went surprisingly good. I did 9/9 pour/no pour, and (the same) 9/9 pressure cooker/oven. Along with that did an additional batch of 20+ overall filled different glasses some days later. Approximately one third of all dishes and glasses were wrapped in tin foil. I only had an issue with a stack of 4 dishes which were blank, filled, blank, filled without the foil. They first were so stuck, that I had decided to let them be for some days. Then this stack landed, suited in a plastic bag, in the SAB. Finally I got them apart in the SAB without effort and without hot water. Was quite an experience.

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Re: Glass Petri Dishes sterilisation [Re: Putrema]
    #27674452 -

i also use glass dishes, i do them exactly like you would any no pour agar tek, wrap them in foil & PC for 45 minutes. i leave them in the tinfoil & put them in the SAB after they cool, then unwrap them & let them dry for a bit. if you put a tshirt or something over the holes in the SAB you can leave them to fully dry for awhile, but i usually wrap them with some condensation still on the plates. they usually dry off after i use them for transfers. i’ve never sterilized & then poured them, but if i did i’d honestly just sterilize in the oven to avoid condensation & stuff in the plates. if you’re going through the trouble of pressure cooking them i feel like you might as well just make them a no pour plate while you’re at it.


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Dishes sterilisation [Re: hazyhorse]
    #27674462 -

You talking about that vacuum lock when they get wet? Hate that dammit!

The spotting from wet plates is annoying too. You even need to hand dry glass plates when you wash them or they get spotty.

That's why I prefer the oven. It's nice and convenient to do stacks of 6-8 glass plates wrapped in foil.

Check out my glass petri tek if you havent seen it. Some goober kinda clogged it up and set me off, but I hope you still find it helpful.

Also worth noting on pouring glass plates: Don't stack them too high, especially if you have a lot of vibrations on your hood. My commercial hood is perfectly balanced and any higher than about 10 and they start to dance on the rack over time. YMMV.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Dishes sterilisation [Re: sandman420] * 1
    #27674503 -

i’ve found some white vinegar during the washing/PC process helps a lot with spotting!! still a lot of condensation with the PC though. the thought of pouring plates in an SAB has always been daunting, but if i ever get the urge i’ll def be referring to your tek!!


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Dishes sterilisation [Re: hazyhorse]
    #27674515 -

personally if I just had a SAB I would do no pours. That's just me, but I've had some troubles with pouring in a SAB cause I suck in a SAB.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Dishes sterilisation [Re: sandman420] * 1
    #27674524 -

yeah same here, which is why i haven’t poured plates yet lol. i would love to if i had a flowhood, but that’s an investment i’m not sure i want to make right now lol


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Dishes sterilisation [Re: hazyhorse]
    #27674563 -

Oh come you guys! Oven bake for pristine plates.
Get yer pouring technique down in da SAB!
My last run had zero auto contams two weeks out.

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Re: Glass Petri Dishes sterilisation [Re: Inthepit] * 1
    #27674569 -

i do my glass plates in the oven. when i've done them in the pc they come out wet between plates and causes a suction effect that makes some bottoms and covers stick together like super glue. in the oven they come out dry and easier for me to handle.

:2cents:



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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Dishes sterilisation [Re: LotKid]
    #27674582 -

Oh Mommy that's an awesome flowhood!
          :goodnews:
It's got the flow right down at the table, no lip!

Edited by Inthepit (02/26/22 07:31 PM)

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Re: Glass Petri Dishes sterilisation [Re: LotKid]
    #27674584 -

i’ve definitely had that suction cup action happen with an agar transfer in the other hand… really annoying to shake the lid off mid transfer lol. i can get them to dry out if i leave them to sit in the SAB for like a day (with the arm holes covered), but i usually don’t like leaving it up for more than a few hours. i’ll have to try my hand at an SAB pour one of these days.


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Dishes sterilisation [Re: hazyhorse]
    #27674589 -

Those plate carriers or whatever you call them are so cool lotkid I need to get some of those.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: Glass Petri Dishes sterilisation [Re: sandman420]
    #27674600 -

yeah where did you get those?? if i don’t have to wrap them in tinfoil every time i cook them i’d rather not lol


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: Glass Petri Dishes sterilisation [Re: sandman420] * 1
    #27674601 -

scored mine used on ebay


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Dishes sterilisation [Re: LotKid]
    #27674602 -

OH YOU DOG!

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Re: Glass Petri Dishes sterilisation [Re: LotKid]
    #27674607 -

https://www.ebay.com/itm/191770243716?hash=item2ca665b284:g:1ggAAOSwJcZWgygE

here's new ones :shrug:


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Glass Petri Dishes sterilisation [Re: LotKid]
    #27674615 -

Pour and store. I hate spraying down my medium tote full of poured plates with alcohol so I can open it in the hood. This would be very handy. :whacker:


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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North Spore Shop: Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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