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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tri]
    #29715029 -

Im making agar for the first time

Afte sterilizing for 45 minutes at 15psi the bottle came out with quite some sediment at the bottom, is this normal? I dont have a pic of that cos my instinct was to give it a shake. Looks decent now I think.

Also color changed a bit after the PC but I think thats normal. Liked the color before PC a lot more  :tongue:

Used Stipe-n Cap recipe which was:

600ml distilled h20
12g agar
12g Dry malt extract
(I added blue food coloring)

Before PC


After PC


looking ok?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin] * 2
    #29715035 -

Looks great, sediment is fine and normal, a gentle swirl not a shake or you'll get bubbles lol


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mindexlpora] * 2
    #29715057 -

Pleasure to make you're acquaintance fellow shroomer :hatsoff::mushroom2: welcome to the shroomery! Thanks for sharing what you've got going on. The first plate looks like mold to me as well, it's growth pattern is very disorganized. How long ago did you inoculate the plate?

Have you got anymore plates going?

Prints are inherently a little dirty and sterile technique certainly takes awhile to dial in, so hang in there! Stick with it and I can assure you success will come your way. Good call starting out with agar, it will serve you nicely:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cronicr] * 4
    #29715058 -

Ok first pour ever done. Lets see how this goes, contam and condensation wise. I didnt burn myseld neither did I make a mess, in my books thats already a win.

I will share my process here for those who are like me and like to learn through photos.

What I used:


weighted 12gr of Dry light malt extract


Added to a jar


Then poured 600ml of distilled water


Then using a funnel and a coffee filter, filtered the mix. I used 2 coffee filters.


Weighted 12gr of Agar Agar


Added the Agar Agar to the filtered mix


Stirred well then added blue food coloring (optional)


Sent to the PC for 45 minutes at 15psi




And this is after the PC


Waited til it was at around 60 Celsius and took it to the SAB


Poured the plates and now lets hope for the best


Left the stacks inside the SAB cooling down and to let some of the condensation to evaporate. Will wrap them in a bit.

Some plates, specially the first ones, I poured waaay too much. Learning curve.

I will wrap them up now and chuck them in a container and wait a few days to see if any contam shows up.

Fun process, have to admit that I was a bit shaky when pouring the first ones, mostly nervous about making a mess and having to clean up rather than scared of burning myself.

If anyone spots anything that is a no no, and has any advice to share... please do so!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin] * 1
    #29715068 -

Nicely done 0_tonin!:mushroom2: cool color plates as well.:mushroom2:whatcha putting on agar?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin]
    #29715078 -

0_tonin said:
Ok first pour ever done. Lets see how this goes, contam and condensation wise. I didnt burn myseld neither did I make a mess, in my books thats already a win.

I will share my process here for those who are like me and like to learn through photos.

What I used:


weighted 12gr of Dry light malt extract


Added to a jar


Then poured 600ml of distilled water


Then using a funnel and a coffee filter, filtered the mix. I used 2 coffee filters.


Weighted 12gr of Agar Agar


Added the Agar Agar to the filtered mix


Stirred well then added blue food coloring (optional)


Sent to the PC for 45 minutes at 15psi




And this is after the PC


Waited til it was at around 60 Celsius and took it to the SAB


Poured the plates and now lets hope for the best


Left the stacks inside the SAB cooling down and to let some of the condensation to evaporate. Will wrap them in a bit.

Some plates, specially the first ones, I poured waaay too much. Learning curve.

I will wrap them up now and chuck them in a container and wait a few days to see if any contam shows up.

Fun process, have to admit that I was a bit shaky when pouring the first ones, mostly nervous about making a mess and having to clean up rather than scared of burning myself.

If anyone spots anything that is a no no, and has any advice to share... please do so!


:snoopyes:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge]
    #29715082 -

Agar Wedge said:
Nicely done 0_tonin!:mushroom2: cool color plates as well.:mushroom2:whatcha putting on agar?



Thanks mate!

I plan to test all the LC jars Ive done. Then transfers.

It was mostly to learn the process and so from now on I can do my own instead of buying poured plates which is what I was doing until now (they were a bit pricey).


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge] * 1
    #29715094 -

Hi thankyou for the warm welcome!

I inoculated them 6 days ago. Yes having read more of this thread and the link @tri posted, I don't see any of the hallmark signs of healthy mycelium, although plate 2 was looking so hopeful yesterday. Would you say plate 2 was mycelium that became contaminated?

I have 1 more MEAG plate and 5 MEA. This evening I will inoculate the last MEAG plate and an MEA plate and will try hard to improve my SAB workflow and technique. Cheers.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin] * 1
    #29715097 -

0_tonin said:
Im making agar for the first time

After sterilizing for 45 minutes at 15psi the bottle came out with quite some sediment at the bottom, is this normal?


Welcome to the wonderful world of pouring agar :lol:


--------------------
𝕴𝖋 𝕴 𝖍𝖆𝖛𝖊 𝖘𝖊𝖊𝖓 𝖋𝖚𝖗𝖙𝖍𝖊𝖗 𝖎𝖙 𝖎𝖘 𝖇𝖞 𝖘𝖙𝖆𝖓𝖉𝖎𝖓𝖌 𝖚𝖕𝖔𝖓 𝖙𝖍𝖊 𝖘𝖍𝖔𝖚𝖑𝖉𝖊𝖗𝖘 𝖔𝖋 𝕲𝖎𝖆𝖓𝖙𝖘

:drugnerd: The TekDek v.2    Autist Tally :laugh:

:mushroom2:How to make a TEK:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin] * 3
    #29715228 -

How long did it take you to upload those 13 pictures in your post? Lol

Also, stir plate before and after PC will get rid of the sediment

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers] * 1
    #29715814 -

I suck at agar. My plates are never clean and I don’t understand why. The last one I had I was told to cabin sequester the good part, managed to get 4 plates out of it. Only one survived





The contam showed up sometime between this morning and right now, I’m gonna try to get the piece that looks good and put it on a plate…should I cabin sequester it? Should I try to get a few plates out of that? Should I do it right now or can I wait a day? I have to make more agar

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dj_karma_magnet] * 3
    #29715825 -

That contamination was most likely introduced during your pouring or during your transferring. You just need to work on your techniques dude. There's probably some videos on it. If you're using a SAb, no fast movements, eliminate all of your drafts, don't move anything over the top of the plate, including your hand. Hold the plate sideways if you have to when you place your wedge in there.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dj_karma_magnet] * 1
    #29715835 -

Quote:
I have to make more agar


:letsgo:


--------------------
Learn it, know it, live it


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Re: AGAR ENVY! (Anything and All things agar!) [Re: mindexlpora]
    #29715872 -

Yeah man, always happy to see a new face around here:smile::mushroom2:

I would guess that bitch plates were simply contaminated. 6 days isn't very long in spore germination terms. Fresh spores will show signs of growth in a couple days. However, if they are not fresh, you can expect 6 days just to begin germinating. Are you making your own agar?

If not, it will be to your advantage to learn how to. Doing so will allow you the benefit of unlimited practice, until you get your technique dialed in.
Here's a great Tek

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers] * 1
    #29715883 -

Thanks, I’m using a flow hood but it’s not a very big one. I’m gonna try to cook up some more agar tonight

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dj_karma_magnet] * 3
    #29715891 -

You got this homie:hug::mushroom2:

I'm pretty pumped, this plate that I left in my attic is coming to life. Its purple mystic. Thought it was done for considering it sat for 4 months in temperatures, at times reaching well over 100. Here it is, coming back to life :mushroom2:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Agar Wedge] * 4
    #29719614 -

Made some Ochras transfers today. Also Enigma LC into agar, Flipzilla LC into agar and one single swab of Thai Pink Buffalo into agar.

First time using my own poured plates, feels good. Its been 4.5 days since making the plates, they looked clean so I gave them some use.

Ochras x6
Enigma x6
Flipzilla x6
Thai Pink Buffalo x1







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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0_tonin] * 5
    #29720211 -


Pretty sexy for a T0
:awedance:


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🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


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Re: AGAR ENVY! (Anything and All things agar!) [Re: fontinalis666] * 3
    #29721206 -


This cutie walked out of cabin...into wasteland, I forgot to add karo while making PDA:muppet:
I used this transfer opportunity to practice faht's areal scoop


--------------------
Some say we'll see Armageddon soon
I certainly hope we will
:madpirate:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Reishi Uchiha] * 1
    #29721481 -

All of my agar is fucked. I’m on like t3 with 5 plates and they all are bad cobweb contam. Scrapping everything and starting over.


Will air circulation in the house (heat) cause contam? I am working with a flow hood (it’s small, the guy I got it from claims that he just put a new filter in)

The room I am doing this in is messy…grains on the ground from previous grow etc. I am going to vacuum and try to sterilize the fuck out of everything several times before I do this, plan on putting an air purifier In the room several days beforehand.


I will be doing spore needle to agar plate, any tips of tek would help, really really sick of wasting time and money on this

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