Huskies said:

I have two lil jar containing left over spore solution . Is mixing them together then putting it in one syringe a viable method for crossing if sent to cakes?

Obviously many fruits might not be crosses, but if they are distinct phenos it should be east to identify when a cross happens.

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fontinalis666 said:

LITFA

Leave it the fuck alone, looks like its chugging along fine

Edit: read your earlier post, sounds like you're wondering when to start "fruiting" it, you can introduce "fruiting conditions" from the getgo when spawning to a tub. Covering the holes for colonization is an outdated way of doing things. Open that sucker up and let it breathe, you have knots, it wants to grow

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langten69 said:

speedspawn said:

Hey man thanks, you're not wrong about both the knots and pinning. Another gentleman before you suggested to wait a few days though. Hope these images are better, thanks for the help.

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Click my link where it says "MODERN FRUITING CONDITIONS" in my signature. It will explain why fontinalis666 says to LeaveItTheFuckAlone (LITFA)

You're doing great BTW




EDIT: Like fontinalis666 says, if you have been depriving the tub of oxygen...open that bad boy up

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langten69 said:

Huskies said:

I have two lil jar containing left over spore solution . Is mixing them together then putting it in one syringe a viable method for crossing if sent to cakes?

Obviously many fruits might not be crosses, but if they are distinct phenos it should be east to identify when a cross happens.

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This is possible, it depends on how long the jars have been sitting because if it has been long, then most of the spores should have started germinating

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auroradawn said:

Tips for agar getting stuck to scalpel? Had a very frustrating experience today, the wedge just wouldn't come off into the grain jars 😭.

Bigger wedge size or higher agar % in recipe maybe?

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You can use the inside jar rim to get the wedge off

auroradawn said:

Tips for agar getting stuck to scalpel? Had a very frustrating experience today, the wedge just wouldn't come off into the grain jars 😭.

Bigger wedge size or higher agar % in recipe maybe?

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fontinalis666 said:

auroradawn said:

Tips for agar getting stuck to scalpel? Had a very frustrating experience today, the wedge just wouldn't come off into the grain jars 😭.

Bigger wedge size or higher agar % in recipe maybe?

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Someone posted a good vid in agar envy recently that helped me a lot, I can try to find it, but basically when you grab your wedge you want to have the sharp edge of the scalpel blade oriented downward, and when you make your transfer you push down and then pull out, so you slice through the bottom of the wedge and slightly into the new plate underneath which gives the wedge a lot less purchase on the blade and it shouldn't stick

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She's talking about inoculating a jar though.

fontinalis666 said:

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No worries though, I got to post my hands again

PuffM4gicDr4gon said:

I find that not letting your scalpel cool off causes the agar to stick. If you have a clean section on your donor plate you can stab it a couple times to cool it off a bunch. Or just sacrifice a plate. Or just let it sit in front of your FFU if you have one.

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Kloakk said:

fontinalis666 said:

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No worries though, I got to post my hands again

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Those sexy Scandinavian working hands

Smellyhobbit said:

PuffM4gicDr4gon said:

I find that not letting your scalpel cool off causes the agar to stick. If you have a clean section on your donor plate you can stab it a couple times to cool it off a bunch. Or just sacrifice a plate. Or just let it sit in front of your FFU if you have one.

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2 scalpels also solves this problem. You always have a cool blade if you alternate between two.

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Sheeeeit. I have two scalpels.

Churpee Choozle said:

@Hip: I used rye for many years, used whole brown rice, spelt, oats and millet. Oats were the only ones I straight out rejected after the first batch. What a bitch of a grain. Maybe it's the grain, not the prep.

The thing with millet is, if some kernels burst it doesn't get messy. Doesn't lump half as much as the larger cereals

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Huskies said:

langten69 said:

Huskies said:

I have two lil jar containing left over spore solution . Is mixing them together then putting it in one syringe a viable method for crossing if sent to cakes?

Obviously many fruits might not be crosses, but if they are distinct phenos it should be east to identify when a cross happens.

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This is possible, it depends on how long the jars have been sitting because if it has been long, then most of the spores should have started germinating

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Well gonna see what happens, KSSS x Wild Australian Murbah, KSSS X Great White Monster, CRS x Wild Australian Murbah.

I tried noccing up some of the straw cakes I just made with the spore solution which is hubris. Most of them were inoculated with Sibu LI, got a few more left for Enigma.

If they pan out, the pheno differences should be notable enough to clue in on the right ones.

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Im excited to see if you get some crosses

Fiet said:

I Pre prep my quart jar yerday with Rye. I boil the Rye Grains for 40min in Water then i let it cool down so the Vapor is gone for that a use a layer of Backingpaper for the Rye Grains so that they can dry a bit. I filled 2 quart jars with 250g dry rye after Boil the Hydrate so the Jar looks half filled. Then i pressure cooked both Jars for 90 min at 15PSI let it cool over night with the Aluminum Foil cover on the lid. The lid a prepare with a Inception port and a 4mm Hole I screw it and cover the hole inside and outside the lid with a layer of micropore Tape.

The Liquid Culture i make 4 Day's ago looks Lotte cloudy but the Mycelium growing Super quick i give it every Day a shake with a magnet sirerer.

I think next week i think the LC is ready.. I know you will say test on Agar first I will but also will test it on my Grains at the same time so no time too loose when agar looks good i know the Grains are too.

What you think?

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bloodssonnew said:

I'm not very experienced with lc though the ones that where clean I seen on this site where clear in the jar not cloudy 😅 so maby test that for sure on agar

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