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Re: Ask Quick Questions, Get Quick Answers [Re: Multimeter]
    #29675891 -

If you’re talking about agar plates...

Check out Agar Envy


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍢
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION πŸ§ͺ
JOIN CAKE WARS III TODAY 🫡
MODERN FRUITING CONDITIONS πŸ„
REVITALIZE THE CUBENSIS GENEPOOL πŸ₯Š
MY FAVORITE TEKS πŸ“–

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Re: Ask Quick Questions, Get Quick Answers [Re: Multimeter]
    #29675892 -

Multimeter said:
Question,

Where is the thread for research plates and how to start from them? Thanks!


Have you read the Hitchhiker's Guide yet? It's the perfect place to start if you're brand new, that's where I learned about agar


--------------------
idrk how I got here or what I'm here for but I  keep waking up with this biological urge to not die✨

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29675898 -

Hello, what do you guys think of this tub? Does it look ready to fruit or should I wait a few more days? Please also tell me if the surface conditions look okay?

Thanks


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Re: Ask Quick Questions, Get Quick Answers [Re: speedspawn] * 1
    #29675901 -

It looks good, you just need to give it time to fully colonize the substrate, maybe a few more days.

If you prepped the substrate correctly, it should be at what's called "field capacity"

If your substrate is at field capacity,  it will start to produce countless water droplets on the surface by itself after it has fully colonized, without you having to mist it

If all of that was done, you did a perfect job and mushrooms will pop out when they are ready to

Welcome to the shroomery


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍢
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION πŸ§ͺ
JOIN CAKE WARS III TODAY 🫡
MODERN FRUITING CONDITIONS πŸ„
REVITALIZE THE CUBENSIS GENEPOOL πŸ₯Š
MY FAVORITE TEKS πŸ“–

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Re: Ask Quick Questions, Get Quick Answers [Re: langten69]
    #29675907 -

I bought some sterilized grain bags from an allegedly trusted vendor. They sell supplies for gourmet mushrooms, but grain is grain.

I grabbed some bags and this is what arrived:




I contacted the vendor and asked what was up with all the residue in te bags. They said they add sawdust to catch excess water from the grains. I said I was not a fan, that properly hydrated grain shouldnΒ΄t release water. And I said that I felt like this sawdust thing was cutting corners.

Was I a dick?

I ended up getting a full refund once I showed them some contaminated bags.

Question is: is this sawdust bs a thing? Was I a Karen? Should I just toss all the bags or try to inoculate the ok looking ones?

Thanks guys!

One of the contam ones:



--------------------
How about the racist, sexist, phobia soup we've all been drowning in because of him? And I'm not just talking about Jesus. I'm talking about all organized religion - exclusive groups created to manage control, a dealer getting people hooked on the drug of hope, his followers nothing but addicts who want their hit of bullshit to keep their - their dopamine of ignorance, addicts afraid to believe the truth - that there is no order, there's no power, that all religions are just metastasizing mind worms meant to divide us so it's easier to rule us by the charlatans that want to run us.

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Re: Ask Quick Questions, Get Quick Answers [Re: langten69]
    #29675915 -

Yes the substrate is at field capacity. I'll report back after a few days.

Thank you.

Edited by speedspawn (09/23/26 05:49 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: 0_tonin]
    #29675916 -

0_tonin

If the bag is already contammed when you get it,  you should get a refund for it

Although im not entirely sure I see contam

Im assuming they have a self healing injection port for liquid culture syringe

Go ahead and innoc the ones that dont have contam

sawdust is technically for woodlovers so it might not be best for dung lovers but it could work depending on what your growing


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍢
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION πŸ§ͺ
JOIN CAKE WARS III TODAY 🫡
MODERN FRUITING CONDITIONS πŸ„
REVITALIZE THE CUBENSIS GENEPOOL πŸ₯Š
MY FAVORITE TEKS πŸ“–

Edited by langten69 (09/23/26 05:52 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: langten69]
    #29675921 -

No injection port, just a filter for breathing.

If you zoom in at the bottom of the bag there some contaminated grains for sure, not so obvious in the picture because when handling the bag some grains ended up buried and out of sight. But theres contam for sure.

I Think I will just toss them, I donΒ΄t have lots of LC to be wasting it in sus looking bags.

how risky is inuculating with a syringe poking the bag and then placing some micropore or duct tape in the hole? Of course inside a sab, sterilized needle, ISO sprayed on bag and so on.

Thanks bud


--------------------
How about the racist, sexist, phobia soup we've all been drowning in because of him? And I'm not just talking about Jesus. I'm talking about all organized religion - exclusive groups created to manage control, a dealer getting people hooked on the drug of hope, his followers nothing but addicts who want their hit of bullshit to keep their - their dopamine of ignorance, addicts afraid to believe the truth - that there is no order, there's no power, that all religions are just metastasizing mind worms meant to divide us so it's easier to rule us by the charlatans that want to run us.

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Re: Ask Quick Questions, Get Quick Answers [Re: Multimeter] * 1
    #29675923 -

Multimeter said:
Question,

Where is the thread for research plates and how to start from them? Thanks!


Check out Stipe's Why agar? thread

Bod's Comprehensive Agar Tek is also excellent


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: bluescreen]
    #29675935 -

0_tonin

Its slightly risky but should work if your sterile technique is on point

Im not gonna tell you to buy more stuff but you'll have the best chances if you get self healing silicone and apply it to the bag as a SelfHealingInjectionPort (SHIP)

There is a slight chance that duct tape has contamz; ive seen applying micropore to the injection hole work, but that was on some uncle bens tek shit which we politely shun on this forum (even tho it can still work sometimes)

Like i said ive seen it work so its worth a shot


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍢
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION πŸ§ͺ
JOIN CAKE WARS III TODAY 🫡
MODERN FRUITING CONDITIONS πŸ„
REVITALIZE THE CUBENSIS GENEPOOL πŸ₯Š
MY FAVORITE TEKS πŸ“–

Edited by langten69 (09/23/26 06:47 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: langten69] * 1
    #29675938 -

If using lc or mss to inoculate make sure ur filter is up higher than the lvl of grains to prevent potential wetness getting on your parafilm or micropore or whatever :smile:

If using some kinda tape like that unravel a fair bit and cut it and toss that first piece in the trash. :smile: just to increase chances of success lol


--------------------





An enigma some caerulescens n a mono 😎

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Re: Ask Quick Questions, Get Quick Answers [Re: speedspawn]
    #29675956 -

speedspawn said:
Hello, what do you guys think of this tub? Does it look ready to fruit or should I wait a few more days? Please also tell me if the surface conditions look okay?

Thanks




Welcome, speedspawn! :hi:

Fruit it β€” it's practically begging you to. If I'm not mistaken, you've got pinning or early hyphal knots there.

And if you can, post a closer, more detailed photo of the substrate. It's hard to gauge the humidity from this picture.

Hugs :heart:


--------------------
"Little shark in da bathtub"

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Re: Ask Quick Questions, Get Quick Answers [Re: bloodssonnew]
    #29675962 -

Thanks guys. I will be starting to use jars with injection ports soon, just waiting on my PC and gas burner to be delivered. And a 20kg bag of oats should be here tomorrow.

But since I have these grain bags I thought it could be worth a shot... but the more I think about it the more I want to save my LC and agar for sawdustless grain.

Basic questions about PC runs:

Β·Jars can touch each other but not touch the walls of the PC, right?
Β·When stacking two layers of jars, having just a separator (with lots of holes, like one of those pizza trays) between the two layers is good enough? My instinct tells me that ideally there has to be a bigger gap for steam to pass through more freely.
Β·Whats the general consensus on PP5 plastic jars for spawn? How well do they tolerate the PC cycles? Do they end up giving up and losing the tightness on the twist lids?

Cheers guys





--------------------
How about the racist, sexist, phobia soup we've all been drowning in because of him? And I'm not just talking about Jesus. I'm talking about all organized religion - exclusive groups created to manage control, a dealer getting people hooked on the drug of hope, his followers nothing but addicts who want their hit of bullshit to keep their - their dopamine of ignorance, addicts afraid to believe the truth - that there is no order, there's no power, that all religions are just metastasizing mind worms meant to divide us so it's easier to rule us by the charlatans that want to run us.

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Re: Ask Quick Questions, Get Quick Answers [Re: 0_tonin]
    #29675982 -

I have two lil jar containing left over spore solution . Is mixing them together then putting it in one syringe a viable method for crossing if sent to cakes?

Obviously many fruits might not be crosses, but if they are distinct phenos it should be east to identify when a cross happens.


--------------------
I call them Huskies cause they Mush!


Huskies' Grimoire
The Official Wild Cubensis Thread
Easy Instant Pot Agar
Let's Talk Cowtek

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Re: Ask Quick Questions, Get Quick Answers [Re: Huskies] * 1
    #29675984 -

Ive grown a lot of shrooms wirh hollow
Stipes for years.  Ofc solid stipes are ideal, but theres nothing inherently wrong with hollow guys.  Fp+, charlie brown, sGT, are all hollow and thems are awesome cultures.


--------------------
The Hitchhiker's Guide to the Shroomery
Visit our cultivation museum
The Hitchhiker's guide Pt. 3

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Re: Ask Quick Questions, Get Quick Answers [Re: Belintaf]
    #29675992 -

Hey man thanks, you're not wrong about both the knots and pinning. Another gentleman before you suggested to wait a few days though. Hope these images are better, thanks for the help.








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Re: Ask Quick Questions, Get Quick Answers [Re: speedspawn] * 1
    #29675995 -

LITFA

Leave it the fuck alone, looks like its chugging along fine

Edit: read your earlier post, sounds like you're wondering when to start "fruiting" it, you can introduce "fruiting conditions" from the getgo when spawning to a tub. Covering the holes for colonization is an outdated way of doing things. Open that sucker up and let it breathe, you have knots, it wants to grow


--------------------

Edited by fontinalis666 (09/23/26 08:52 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: speedspawn] * 1
    #29675998 -

speedspawn said:
Hey man thanks, you're not wrong about both the knots and pinning. Another gentleman before you suggested to wait a few days though. Hope these images are better, thanks for the help.










Click my link where it says "MODERN FRUITING CONDITIONS" in my signature. It will explain why fontinalis666 says to LeaveItTheFuckAlone (LITFA) :thumbup:

You're doing great BTW

:leocheers:


EDIT: Like fontinalis666 says, if you have been depriving the tub of oxygen...open that bad boy up


--------------------
"Why try for high fives,
When my pies have nine lives?"
DUBJUG TEK 🍢
PROJECT H.D. 🐴
PLASTIC-EATING MYCELIUM 🍴
TRANSGENETIC EXPRESSION πŸ§ͺ
JOIN CAKE WARS III TODAY 🫡
MODERN FRUITING CONDITIONS πŸ„
REVITALIZE THE CUBENSIS GENEPOOL πŸ₯Š
MY FAVORITE TEKS πŸ“–

Edited by langten69 (09/23/26 09:07 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: bloodssonnew]
    #29676017 -

I Pre prep my quart jar yerday with Rye. I boil the Rye Grains for 40min in Water then i let it cool down so the Vapor is gone for that a use a layer of Backingpaper for the Rye Grains so that they can dry a bit. I filled 2 quart jars with 250g dry rye after Boil the Hydrate so the Jar looks half filled. Then i pressure cooked both Jars for 90 min at 15PSI let it cool over night with the Aluminum Foil cover on the lid. The lid a prepare with a Inception port and a 4mm Hole I screw it and cover the hole inside and outside the lid with a layer of micropore Tape.

The Liquid Culture i make 4 Day's ago looks Lotte cloudy but the Mycelium growing Super quick i give it every Day a shake with a magnet sirerer.

I think next week i think the LC is ready.. I know you will say test on Agar first I will but also will test it on my Grains at the same time so no time too loose when agar looks good i know the Grains are too.

What you think?





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Re: Ask Quick Questions, Get Quick Answers [Re: langten69] * 3
    #29676018 -

GOOD MORNING EVERYONE

I love you all and am thankful for all your support and willingness to help one another.

:heart:


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

Edited by Lo-Z (09/23/26 07:13 PM)

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Shop: Mushroom-Hut Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   North Spore Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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