DERRAYLD said:

Buy glass rather.

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DERRAYLD said:

Limit your work and it will help to improve focus as well.

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Solid OG advice from an OG. You're right. Perhaps I'll suck it up and buckle down.

Ovoidhunter said:

What kind of plates do you guys use. I need to order some and looked and they seem pricey. I need 20-50 plates for my aspirations I'd guess but I don't want to buy plates made of cheap material that won't survive long term use. Please point me in the right direction. I am so tired of pasty plates.

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Blizzloo said:

Good morning everyone

Can i use window cleaner instead of water with drop of soap to spray my SAB?

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Blizzloo said:

My question comes from this tek Bods sab tek
He's talking about spraying soapy water. Is this outdated information and nobody does it anymore?

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Blizzloo said:

Thanks for all the contradictory answers
I'm also asking because I already have window cleaner in a spray bottle, and if I'm only supposed to use soap and water, I'll have to get an extra spray bottle, which will take a few days.

So if it's only meant to catch microbes, then perhaps the question should be, can window cleaner be harmful?

To be clear, I use ISO 70 and I use it a lot. I'm not asking if I can use window cleaner to sanitize or so, but if I can use it instead of soap and water.

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B Traven said:

That's why crazy motherfuckers like Kloakk can get away with running a dry SAB. Careful technique, a clean location, and a perpetually undisturbed SAB is all you REALLY need. If you're a crazy motherfucker like Kloakk, that is.

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Blizzloo said:

That explains a lot.

Kloakk said:

you should be able to find a spray bottle with premade dishwash soap, that will work.

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we dont have that in country I live, but no problem.

PuffM4gicDr4gon said:

If you're in a pinch, put the window cleaner in something else, rinse it out, and replace with soapy water

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Thats also an idea. Thx

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BeefExtreme said:

Theyre tropical
Species.  Youll
Be fine but dont choke it.  Make sure you have airflow or youre sure to trich out

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Has happened to me a couple of times. Desert highs will only last for a couple of hours per day this time of year. I have seen them start to struggle if indoor temperatures are in the low 90s for more than a few hours a day, but they can take it for a little while.

If you can, place the tubs and jars on a tile floor in the lowest or coolest part of your home. You should be fine.

Nokumana said:

I need more autoclavable polycarbonate 90mm petri dishes!  Amazon is out of the ones I like (Viabooth), unless I want to buy a 500 pack, and I don't need that many.

Is anyone able to share a different, reliable source? 

I really don't want to use single use petri's, and glass is too pricey. I suppose I could suck it up and just get the 500 - and give away/sell some?  But I want to KIS (keep it simple).

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DERRAYLD said:

Meh just use soapy water.

Let's not go back to days of lysol.

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Nokumana said:

I need more autoclavable polycarbonate 90mm petri dishes!  Amazon is out of the ones I like (Viabooth), unless I want to buy a 500 pack, and I don't need that many.

Is anyone able to share a different, reliable source? 

I really don't want to use single use petri's, and glass is too pricey. I suppose I could suck it up and just get the 500 - and give away/sell some?  But I want to KIS (keep it simple).

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Have you tried the 60mm ones?

I bought a lifetime supply of those and haven't looked back. (OK, OK, every once in a while I wish I had some bigger ones. But then I just pour a sleeve from the unfinished case of 90mm disposable dishes I have, the itch is scratched, and I remember why I actually do prefer the smaller ones.)

The 60mm dishes have some pretty nice advantages:

- They take up a lot less space
- Agar media goes a lot further (only about 10 ml per dish makes quite thick plates)
- Their size is perfect for a wide mouth mason jar. Quart jars are convenient because a whole 10-dish sleeve fits in one, but after a few rounds I switched to pints because they are more space efficient overall. I fit 8 in a wide mouth pint and usually sterilize and pour multiples of 5 jars (40 dishes, one 400ml batch of agar media). The poured plates can go back in the jars unwrapped. I used to try leaving uninoculated plates unwrapped in Ziploc bags but always fucked up handling them; never have I contaminated any in a pint jar, though I usually do use up a jar within a couple of sessions. I like to use a strip of parchment as a sling and keep them upright in an upright jar. Someone suggested skipping the parchment and stacking them on the lid, but I didn't like the stability of upside down jars and my fingers are too fat to grip the bottom dish to lift the stack - YMMV.

A culture does fill the plate a bit quicker, and you get less mycelium (about half) per plate. But I don't mind that because the plate is still usable for months if thick enough, and small wedges are fine for pretty much everything. (I can get about 5 smash wedges for trades/giveaways out of a plate - that small number is probably the biggest disadvantage for me. But it's easy to make more plates when planning something like that.)