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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29478585 -

tultedspoon said:
Was reading a thread about cloning rehydrated fruits, apparently it is easier than people think. If I take a careful tissue sample from the middle of the biggest fruit I was able to grow, could I try to inoculate some jars with this?



Most times I've seen that it was with cubes. Pans are so small their stipes dry to practically nothing. Getting a sample from the center might be difficult.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon]
    #29478602 -

PuffM4gicDr4gon said:
tultedspoon said:
Was reading a thread about cloning rehydrated fruits, apparently it is easier than people think. If I take a careful tissue sample from the middle of the biggest fruit I was able to grow, could I try to inoculate some jars with this?



Most times I've seen that it was with cubes. Pans are so small their stipes dry to practically nothing. Getting a sample from the center might be difficult.



You can do it with a smallest sample you got by means of tweezers, scalpel and alcohol.

Edited by Noah Keeper (02/09/26 07:48 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: tultedspoon]
    #29478628 -

tultedspoon said:
Some of those bispos look similar to what I was growing, maybe I got bispos afterall like TF originally suggested. A lot of the other pics I see make them look different though.



Just guessing, probably they do looks different depends on  origin, same as other samples collected in different locations under same biological name. But I can tell for sure that this guys is very potent and trip is amazing

Edited by Noah Keeper (02/09/26 08:48 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29478633 -

I rarely clone them but when I do it's water agar for the tissue recovery and a minimum of one cabin to catch ride along bacteria.

Faht has a nice write up for grabbing center tissue that can work well with most phenotypes of cyans (though there can be some variance in stipe structure even within just this one species).

But that wouldn't work with a dried sample.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29478638 -

tree frog said:
I rarely clone them but when I do it's water agar for the tissue recovery and a minimum of one cabin to catch ride along bacteria.

Faht has a nice write up for grabbing center tissue that can work well with most phenotypes of cyans (though there can be some variance in stipe structure even within just this one species).

But that wouldn't work with a dried sample.



Thanks so much 🙏🏻 I noticed that after applying alcohol onto fruit before start cutting it apart to extract samples from inner side helps to minimise bacterial contamination and usually when there are some bacterial spot it’s almost steady or way slower then mycelium what allows me to take  more or less clean sample for transfer

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29478642 -

tultedspoon said:





The pins look like they could be but the fully grown fruit does not.

I believe it's subgenus panaeolina.  I.e. related to cinctulis, but possibly a different species depending on when and where it was found.  Without microscopy or a better bioassay from properly dried fruits who knows :shrug:



--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 6
    #29478653 -






  same cinctulus I colluded in wild but different weather conditions, light color when was a bit dry days a colourful was fall season, lots of rain and colder

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29478655 -

I will start putting together an updated taxonomy guide in my journal soon, and ask a friend of mine to look it over before I move it to Advanced Mycology.

Probably the most helpful thing for us as cultuvators is to have some idea of what subgenus we are working with as it can help us gauge potency.  Most of this thread is now going under bresadolomyces.  Which tend to be tropical (though not always, axfordii is temperate for example) and very strong.

And with new species being observed almost every year and more interest in the genus, it's possible that tulted isn't growing cinctulis but something either unnamed or previously unobserved.

This is a close active relative to cinctulis that isn't named yet for example.

https://www.inaturalist.org/observations/260932110


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29478656 -

I have yet to grow anything other than bresadolomyces (and the inactive species punjabensis).  But I may have to do an outdoor bed of cinctulis this year.

Nice find :smile:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29478666 -

tree frog said:
I have yet to grow anything other than bresadolomyces (and the inactive species punjabensis).  But I may have to do an outdoor bed of cinctulis this year.

Nice find :smile:


thanks 🤝 I haven’t tried them yet😅 visual identification doesn’t really gives solid understanding  what is that for sure.  Interesting job you guys done on inaturalist, thanks for sharing. Are there was any tests of potency or alkaloid content of those paneolina specie ever done?

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29478670 -

:popcorn:


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Everything is better when it is done ON TOP OF A MOUNTAIN!

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29478678 -

The Pandelorian bioassayed them a few times, and that is his grow.  (He's been growing awhile, and I trust his judgement).

Similar range to cinctulis.  The mycelium bruises blue too.

I did try growing it, but it didn't like my setup and the few fruits aborted.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 3
    #29478694 -

unfortunately I haven’t saved many photos, they did grow on same substrate and set up as other pans in my case

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29478696 -

I really want to find mushrooms in nature. I look sometimes but never found a active mushroom ever. Even found False Cesar Amanita parcivolvata several times in different states.

For some reason Panaeolus cyanescens Estro spores germinated faster than Panaeolus bisporus.

I hate my overfilled jars my TTBVI is almost ready to spawn just a couple more grain spots that need mycelium and I should get mushrooms pretty quick.

Im just going to do CVG casing I was planning on doing 72 hours with the lid on to not get fruits. I seen people saying Panaeolus cyanescens turns into mushrooms really fast I remember someone getting pins in 24 hours.

Is that usually the case with them should I not try to build heavy mycelium like with cubensis?


--------------------
I am the master of my fate,
      I am the captain of my soul.

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29478773 -

Foreighn2891 said:





  same cinctulus I colluded in wild but different weather conditions, light color when was a bit dry days a colourful was fall season, lots of rain and colder



:excuseme:

I got that bug for finding wild genetics too. That last one is sexy.

Well fucking done my man, that's the dream there - a pasture of those.


--------------------
Be lucky, may light walk with you.





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Re: Official Copelandia/Panaeolus Thread [Re: CaptainTrips420]
    #29478776 -

CaptainTrips420 said:
I really want to find mushrooms in nature. I look sometimes but never found a active mushroom ever. Even found False Cesar Amanita parcivolvata several times in different states.

For some reason Panaeolus cyanescens Estro spores germinated faster than Panaeolus bisporus.

I hate my overfilled jars my TTBVI is almost ready to spawn just a couple more grain spots that need mycelium and I should get mushrooms pretty quick.

Im just going to do CVG casing I was planning on doing 72 hours with the lid on to not get fruits. I seen people saying Panaeolus cyanescens turns into mushrooms really fast I remember someone getting pins in 24 hours.

Is that usually the case with them should I not try to build heavy mycelium like with cubensis?




so far I have found three different active species, two on the north home, cinctulus or similar to the one we just discussed, pluteus salicinus couple times, and whole trip around Costs rica last end of October gave just cubensis an no any exotics unfortunately

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Re: Official Copelandia/Panaeolus Thread [Re: Noah Keeper]
    #29478828 -

After a number of frustrating attempts to cultivate pan bisporus, I recently tried my hand at cultivating a similar variety: South Africa Mystery. Much confusion over varieties ensued. From what I have researched, there are two different varieties similarly labeled. There is Mystery SA, which is reported to be quite similar to Weza. And there is SA Mystery (or SAM), which is said to be very similar to Pan Bisporus. The latter is the spore print I was supposed to have been provided with. If I post a photo of what I successfully grew, is there anyone who can tell the difference between the two? (Being a klutz, I have had difficulty posting pics before, which I why I ask before again attempting to post a photo)

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Re: Official Copelandia/Panaeolus Thread [Re: blakeyface] * 3
    #29478846 -

Sent six bispo wedges to EF cakes last month, they are slowly doing the thing. The house is too cold for anything to do anything rn. But here's hoping this Spring will bring some fresh pans.

To be completely honest I haven't touched bispo after my maiden voyage last summer. 1.5 Grams was like being navigated through rough seas by Ren and Stimpy. But they are very pretty shrooms.



--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Official Copelandia/Panaeolus Thread [Re: blakeyface]
    #29478853 -

You might not be able to upload photos cause of its size, try to compress it online before upload to 500kb
blakeyface said:
After a number of frustrating attempts to cultivate pan bisporus, I recently tried my hand at cultivating a similar variety: South Africa Mystery. Much confusion over varieties ensued. From what I have researched, there are two different varieties similarly labeled. There is Mystery SA, which is reported to be quite similar to Weza. And there is SA Mystery (or SAM), which is said to be very similar to Pan Bisporus. The latter is the spore print I was supposed to have been provided with. If I post a photo of what I successfully grew, is there anyone who can tell the difference between the two? (Being a klutz, I have had difficulty posting pics before, which I why I ask before again attempting to post a photo)



Compress photos online to 500kb before upload and it will works fine. South African syanescens was ever biggest I had

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Re: Official Copelandia/Panaeolus Thread [Re: Churpee Choozle] * 8
    #29478854 -


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