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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: schmutzen] * 1
    #29443842 -

schmutzen said:
Noob question here...

What is the significance of a plin?

I've had many before and never thought twice about it BUT recently I've had a hell of a time getting a clean plate of hillbillies and noticed a plin (after I dropped it on the floor most likely compromising it).

Should I transfer it?  Put it to grain?  What does it want?

TIA :thumbup:


If you're asking why you got a plin, itthe same answer as any other fruit: it just depends. Could just be that the plate is fully colonized and the genetics of that culture are particularly pinn-y. Could be that there's a contaminant triggering early pinning. Could be lots of things.

I always just transfer them to a new plate, then do a few sequential transfers from the leading edge of growth, then go to grain with it and then see how it all goes. It usually works out great. Especially with Hillbillies.

I'd normally say that a plin represents a potential clone that's already been grown out in a sterile environment, but you mentioned dropping that plate or something, so that might not be the case. Regardless, I'd still do exactly what I said last paragraph with it.

If you got enough plins at one time, then they could also represent the shortest absolute distance to "I just wanna trip."


--------------------


ACAB  🖕🧊


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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29443846 -

BeefSupremeJr said:
it is.  i run cold.  my gf duc is always too warm so we compramise and keep the house at 70 and i wear a sweater.

however, i keep the grow room at around 75. i have tried growing at high 60's and everything just stalls.  cubes are tropical.  mid to high 70s all day long.


Wild.

I don't do that at all. Not keeping direct records, but I bet my stuff is sitting in high 50's to mid-60's for a good chunk of the winter.

Makes me wonder about what we end up selecting for over time, how our Koppen climate classification comes into play, rtc.


--------------------


ACAB  🖕🧊


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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven]
    #29443898 -

Does anyone know what’s going on with this agar?
The inoculation point is live tissue (clone)

From what I’ve seen in forums this isn’t right at all, when I did pk tek the mycelium was ropey and fuzzy but this looks weird I’m thinking bacterial contamination but any advice or help would be appreciated it’s my 1st time cloning/agar work


--------------------
Dorya

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Re: Ask Quick Questions, Get Quick Answers [Re: Kazuya] * 1
    #29443906 -

Kazuya said:
Does anyone know what’s going on with this agar?
The inoculation point is live tissue (clone)

From what I’ve seen in forums this isn’t right at all, when I did pk tek the mycelium was ropey and fuzzy but this looks weird I’m thinking bacterial contamination but any advice or help would be appreciated it’s my 1st time cloning/agar work


Based on the photo that does not look like healthy mycelium.

Healthy mycelium will usually be white and either ropey (rhizomorphic) or fluffy (tomentose) or a combination of both.

What you're growing looks like bacterial contamination and possibly mold.

Did you use a still air box to take the clone? What method did you use to clone it?

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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #29443921 -

I don't have any pictures of my spawn sadly, it was millet if that helps, didn't have any problems in that phase, not that I noticed anyway.

Thanks for the info, as for conditions I've been keeping the internal temp at about 75 DEGF and humidity at 91% according to my hygrometer. Moisture seems good to me, I have beading and the sub is damp and somewhat springy to the touch.  But it's quite possible that what seems good to me is too dry as it's my first go. I'll wait until my fruits have matured then harvest all the pins that have aborted/stalled like you said then give it a heavy misting.  Thanks for the help.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kazuya]
    #29443929 -

Kazuya said:
Does anyone know what’s going on with this agar?
The inoculation point is live tissue (clone)

From what I’ve seen in forums this isn’t right at all, when I did pk tek the mycelium was ropey and fuzzy but this looks weird I’m thinking bacterial contamination but any advice or help would be appreciated it’s my 1st time cloning/agar work


Where did you grab the tissue sample from?


--------------------
Favorite Teks

  ]

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Re: Ask Quick Questions, Get Quick Answers [Re: LotKid] * 1
    #29444021 -

LotKid said:
Q: What do you call a jamaican proctologist?


A: a poké-mon....:jah:


:rofl2:
Welcome back LotKid.


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


Golden Teachers

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Re: Ask Quick Questions, Get Quick Answers [Re: Kazuya]
    #29444233 -

Kazuya said:
Does anyone know what’s going on with this agar?
The inoculation point is live tissue (clone)

From what I’ve seen in forums this isn’t right at all, when I did pk tek the mycelium was ropey and fuzzy but this looks weird I’m thinking bacterial contamination but any advice or help would be appreciated it’s my 1st time cloning/agar work


qq what is actuallly happening


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme] * 1
    #29444272 -

Bacterial tissue transfer. Likely not a sterile tool or outer tissue or any number of things. Good ole contamalam.

Provided it’s only bacteria, this can be saved by taking a conservative sample of the plated tissue, as small as reasonable, and putting it to a BRF puck. Once it’s grown out an inch to an inch and a half, you can easily transfer that back to fresh agar.

But yeah it looks like a technique issue.


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #29444291 -

Hey,
Noob Australian here,
About to move to the fruiting stage of my first grow
I have a 1kg all in one grow bag that is fully colonised
Was going to leave it another week before fruiting it in a SGFC, but my hand has been forced by the formation of pins - it's time to birth!
However, I also noticed some new discoloured patches at the surface of the mycelium, almost yellow/green in colour. 
Can someone more experienced please take a look at these photos?? Could this be considered contamination?? If so, could it just be removed with a knife? I am a little stressed...




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Re: Ask Quick Questions, Get Quick Answers [Re: AwareWithoutSelf]
    #29444344 -

AwareWithoutSelf said:
Hey,
Noob Australian here,
About to move to the fruiting stage of my first grow
I have a 1kg all in one grow bag that is fully colonised
Was going to leave it another week before fruiting it in a SGFC, but my hand has been forced by the formation of pins - it's time to birth!
However, I also noticed some new discoloured patches at the surface of the mycelium, almost yellow/green in colour. 
Can someone more experienced please take a look at these photos?? Could this be considered contamination?? If so, could it just be removed with a knife? I am a little stressed...






Looks like it’s probably just metabolites, so likely not really too much to worry about. I’ve never grown in bags, but if it’s pinning maybe just let her rip?

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Re: Ask Quick Questions, Get Quick Answers [Re: AstroSporeFiend]
    #29444345 -

Senescence question. If a variety doesn’t drop many spores, therefore pretty much needs to be cloned on agar, grown out, cloned again, grown out, etc… is senescence avoided by the fruiting process? Just something I was thinking about earlier.

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Re: Ask Quick Questions, Get Quick Answers [Re: AstroSporeFiend]
    #29444347 -

I"m planning on growing mushrooms via PF tek soon. First time grower.

I've got some 1/2 pint mason jars and a PC on the way. I plan on getting some spores and everything else I need (couple clear tubs, perlite, verm, BRF).

Questions I have -
-Whats a good way to keep the temp up in my closet? I live in a cold climate and keep my heat between 65-70f. I was thinking of getting some grow lights but not really sure how beneficial that would be. Any recs?

-While the myc is growing in the jar, do they need to be kept warm? Would grow/heating lights work to keep them warm or do they need to be kept in the dark?

-I have read of 2 slightly different PF teks. The one where you birth and dunk the cakes after you pop em out of the jar, and then one where you leave the cakes in the jar and just pop the top off and throw it in the SFGC (although in the tek I read, he put his in bags). His benefits to leaving them in the jar is that they need less misting and you don't have to deal with the perlite. However, one big downside, there is lesser surface are for growth. I'd love some opinions on this. (this tek is described in the book Pcilocybin mushroom handbook: easy indoor and outdoor cultivation by LG Nicholas and Kerry Ogame)

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Re: Ask Quick Questions, Get Quick Answers [Re: jeffreykite]
    #29444371 -

Contamination? Scrap the bin? Scrap both? Photos of both bins are a few posts up

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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven]
    #29444373 -

B Traven said:
BeefSupremeJr said:
it is.  i run cold.  my gf duc is always too warm so we compramise and keep the house at 70 and i wear a sweater.

however, i keep the grow room at around 75. i have tried growing at high 60's and everything just stalls.  cubes are tropical.  mid to high 70s all day long.


Wild.

I don't do that at all. Not keeping direct records, but I bet my stuff is sitting in high 50's to mid-60's for a good chunk of the winter.

Makes me wonder about what we end up selecting for over time, how our Koppen climate classification comes into play, rtc.


I also run in the mid 60’s in the winter. It might be a little slower, but I get some fruit. I keep saying I’ll get an oil heater to get the temp up, but still haven’t.


--------------------
Hitchhikers guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: jeffreykite]
    #29444376 -

Ziran’s pf tek

You can go without perlite. Myc dont need light, fruit wants ambient light, or artificial 12/12 cycles, but can do without I believe. Your room temps sound fine for cubensis, but you can put a space heater in a closet if it gets cold...

Welcome:cheers:


--------------------
Learn it, know it, live it


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Re: Ask Quick Questions, Get Quick Answers [Re: xsanriu] * 1
    #29444378 -

xsanriu said:
Contamination? Scrap the bin? Scrap both? Photos of both bins are a few posts up



I only see mushrooms ? Maybe a little dry in there?


--------------------
Learn it, know it, live it


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Re: Ask Quick Questions, Get Quick Answers [Re: Mackload]
    #29444379 -

Are there any tips to encourage agar dish pins?  I have several plates I’ve been waiting on for months but it seems, once everything is colonised, the mycelium just stops growing after a while and eventually the agar dries up too.

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Re: Ask Quick Questions, Get Quick Answers [Re: FlamingFuel]
    #29444388 -

Mycelium bruising or contamination? This was taken this morning after a fairly heavy misting last night.




--------------------
Binary thinking is the best thinking.

plz, tell me exactly what to do :wink:.

If more of us valued food and cheer and song above hoarded gold, it would be a merrier world.

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Re: Ask Quick Questions, Get Quick Answers [Re: FlamingFuel] * 1
    #29444391 -

It's really culture and maybe nutrient dependent afaik, but not guaranteed. Better off transferring or fruiting plates before letting them grow out and dry up if you want fruit imho. But make enough plates and Im sure youll get pins. You could also try ReverendMyc's pucks

Pucks


--------------------
Learn it, know it, live it


Edited by Mackload (01/02/26 08:12 AM)

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Shop: Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Grow Bags

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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