Synack said:

Gilmour Hendrix said:

Synack said:

Pin said:

then transfer from loop to agar

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How, precisely? Keep in mind the goal here is to avoid streaking the plate.

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I was told the opposite. I was told when germinating spores to streak the plate.

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It's hard to "keep state" when a thread has this many unrelated posts/day, but this particular "sub-thread" goes back to https://www.shroomery.org/forums/showflat.php/Number/29335106#29335106 wherein beef suggests that streaking just spreads contam around the plate and is unnecessary, and that he was going to remove streaking from the HHG. I'm testing to see if that approach reduces the contams on these plates.

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Gilmour Hendrix said:

Thank you. I just plopped spores in the middle on my first set of plates and was told I should have streaked. Did some more last night, streaked them, and now that's wrong. I'll figure it out. 2 of 10 had contam on the first plates I did. We will see how it goes on the plates from last night.

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At this point I'm not sure there's a right and a wrong, and if whatever you're doing is working, I wouldn't mess with it. My streaked plates looked like murder scenes, except with bacteria not blood, and this was the outcome of a discussion about where that might have been coming from. Not sure there's broad agreement

Gilmour Hendrix said:

Thank you. I just plopped spores in the middle on my first set of plates and was told I should have streaked. Did some more last night, streaked them, and now that's wrong. I'll figure it out. 2 of 10 had contam on the first plates I did. We will see how it goes on the plates from last night.

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Hah, I just hold the print foil over my plate and scrape some spores with my scalpel, worked every time thus far

BeefSupremeJr said:

not even getting into this. the technique that gets a clean germ plate is the right one gil.

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Kloakk said:

Gilmour Hendrix said:

Thank you. I just plopped spores in the middle on my first set of plates and was told I should have streaked. Did some more last night, streaked them, and now that's wrong. I'll figure it out. 2 of 10 had contam on the first plates I did. We will see how it goes on the plates from last night.

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Hah, I just hold the print foil over my plate and scrape some spores with my scalpel, worked every time thus far

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toadking said:

toadking said:

Making a Jar of LC, I know its a less positively looked on tek but I’m doing 500 ML water 50 Ml corn syrup and 1 gram of peptone with injection port lids, would 2 ccs be enough from my starter culture or should i do 5? possibly even 10?!

Edit: I’m also planning to use the rest on my WBF jars, I have 4 quarts sterilized but only one syringe so I would rather make culture first. Can I keep sterilized jars stored for a bit so my culture develops? Or would I have to sterilize new grain

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Please help

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BeefSupremeJr said:

Kloakk said:

Gilmour Hendrix said:

Thank you. I just plopped spores in the middle on my first set of plates and was told I should have streaked. Did some more last night, streaked them, and now that's wrong. I'll figure it out. 2 of 10 had contam on the first plates I did. We will see how it goes on the plates from last night.

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Hah, I just hold the print foil over my plate and scrape some spores with my scalpel, worked every time thus far

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love it

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It really speaks of the skills the members of this community has when it comes to taking prints, I mean sure, I had to take a couple of transfers, but I'm always doing that with MS anyway.

I'm still baffled by how good you guys are at keeping an eye on the details.

Kloakk said:

BeefSupremeJr said:

Kloakk said:

Gilmour Hendrix said:

Thank you. I just plopped spores in the middle on my first set of plates and was told I should have streaked. Did some more last night, streaked them, and now that's wrong. I'll figure it out. 2 of 10 had contam on the first plates I did. We will see how it goes on the plates from last night.

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Hah, I just hold the print foil over my plate and scrape some spores with my scalpel, worked every time thus far

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love it

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It really speaks of the skills the members of this community has when it comes to taking prints, I mean sure, I had to take a couple of transfers, but I'm always doing that with MS anyway.

I'm still baffled by how good you guys are at keeping an eye on the details.

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toadking said:

Would tap water work fine for an LC solution or does it have to be distilled

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BeefSupremeJr said:

I don't send prints I send smash-wedges. Probably its frowned upon and I don't think the bosses like me talking about it but thats what i do and I've never had someone complain of a contam.

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My first clean(but ain't gonna call somebody out) genetics where wedges, thank you nerdo aka whatisanerd, and they worked like a charm.

I do love prints though, because I get to start fresh and take my own path and the experience along with it. Not going to lie though, man those faht cultures is one hell of a confidence boost. Grow it out on agar, send and canopy

It's just that a drug baggie in my country would take my kids away, and that's pretty scary