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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Synack]
    #29336483 -

Quote:
Synack said: I used a fresh (and freshly flamed) loop to just scrape the print held above the plate,


Hoping you mean above the closed plate


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29336485 -

tryptkaloids said:
Quote:
Synack said: I used a fresh (and freshly flamed) loop to just scrape the print held above the plate,


Hoping you mean above the closed plate


Huh? I was scraping the spores onto the plate..


--------------------
:blackspy:00010010
"When a long train of abuses and usurpations, pursuing invariably the same Object evinces a design to reduce them under absolute Despotism, it is their right, it is their duty, to throw off such Government, and to provide new Guards for their future security."

The Hitchhiker's Guide to the Shroomery <-- This is the way.



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Re: Ask Quick Questions, Get Quick Answers [Re: Synack] * 1
    #29336487 -

The foil isnt clean tho. Only sterile tools and genetic material should be over sterile media. Unless you are using a ffu


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29336497 -

Making a Jar of LC, I know its a less positively looked on tek but I’m doing 500 ML water 50 Ml corn syrup and 1 gram of peptone with injection port lids, would 2 ccs be enough from my starter culture or should i do 5? possibly even 10?!

Edit: I’m also planning to use the rest on my WBF jars, I have 4 quarts sterilized but only one syringe so I would rather make culture first. Can I keep sterilized jars stored for a bit so my culture develops? Or would I have to sterilize new grain


--------------------

Edited by toadking (09/06/25 06:15 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29336501 -

tryptkaloids said:
The foil isnt clean tho. Only sterile tools and genetic material should be over sterile media. Unless you are using a ffu


I'm in front of a flow hood, but ignoring that, what's the right way to get spores from foil to agar? I was under the impression that scraping the print lightly above the plate was an acceptable way?


--------------------
:blackspy:00010010
"When a long train of abuses and usurpations, pursuing invariably the same Object evinces a design to reduce them under absolute Despotism, it is their right, it is their duty, to throw off such Government, and to provide new Guards for their future security."

The Hitchhiker's Guide to the Shroomery <-- This is the way.



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Re: Ask Quick Questions, Get Quick Answers [Re: Synack]
    #29336518 -

Scrape the spores from the foil, away from agar, then transfer from loop to agar. Should be pleeeenty of spores.

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Re: Ask Quick Questions, Get Quick Answers [Re: Pin] * 2
    #29336532 -

:whathesaid: i like to keep the print on a jar to the left of my work (the side opposite my tool hand). Open print, sterilize loop, cool loop, pick up spores and move my tool to the right of my plate, open plate with left hand, deposit spores, done.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Pin]
    #29336534 -

Pin said:
then transfer from loop to agar


How, precisely? Keep in mind the goal here is to avoid streaking the plate.


--------------------
:blackspy:00010010
"When a long train of abuses and usurpations, pursuing invariably the same Object evinces a design to reduce them under absolute Despotism, it is their right, it is their duty, to throw off such Government, and to provide new Guards for their future security."

The Hitchhiker's Guide to the Shroomery <-- This is the way.



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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29336536 -

That's how I do it too lol. I hate loops though and use a scalpel.

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr]
    #29336537 -

cronicr said:
The sediment is completely fine it doesn't effect anything, I often grind wheat in a coffee grinder for agar and it's always there


yea but its not BEUTIFUL cron


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: Synack]
    #29336538 -

Synack said:
Pin said:
then transfer from loop to agar


How, precisely? Keep in mind the goal here is to avoid streaking the plate.


I was told the opposite. I was told when germinating spores to streak the plate.


--------------------



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Re: Ask Quick Questions, Get Quick Answers [Re: the_chosen_one] * 2
    #29336539 -

I do the same but I don’t hate loops or scalpels. I do dislike long walks on the beach.

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Re: Ask Quick Questions, Get Quick Answers [Re: toadking]
    #29336540 -

toadking said:
Making a Jar of LC, I know its a less positively looked on tek but I’m doing 500 ML water 50 Ml corn syrup and 1 gram of peptone with injection port lids, would 2 ccs be enough from my starter culture or should i do 5? possibly even 10?!

Edit: I’m also planning to use the rest on my WBF jars, I have 4 quarts sterilized but only one syringe so I would rather make culture first. Can I keep sterilized jars stored for a bit so my culture develops? Or would I have to sterilize new grain


Follow Up Question- Is a jar of LC required to stay refrigerated?
Also still need answer to first part please_/ \_


--------------------

Edited by toadking (09/06/25 06:25 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: toadking]
    #29336543 -

No. Oddly enough I do refrigerate the syringes :lol:

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao] * 2
    #29336544 -

The Tao said:
I do the same but I don’t hate loops or scalpels. I do dislike long walks on the beach.


Are you not into yoga? Do you have half a brain?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Synack]
    #29336545 -

Synack said:
Pin said:
then transfer from loop to agar


How, precisely? Keep in mind the goal here is to avoid streaking the plate.


Just push the tool into the agar


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29336551 -

The Tao said:
I do the same but I don’t hate loops or scalpels. I do dislike long walks on the beach.


Well, it's not so much a hate thing as a disagreement thing lol. Not fond of the bendy part. Like trying to noc with a doorstop.

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Re: Ask Quick Questions, Get Quick Answers [Re: the_chosen_one] * 1
    #29336554 -

the_chosen_one said:
The Tao said:
I do the same but I don’t hate loops or scalpels. I do dislike long walks on the beach.


Well, it's not so much a hate thing as a disagreement thing lol. Not fond of the bendy part. Like trying to noc with a doorstop.


Homemade loops. Not much bendy in them.

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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix]
    #29336557 -

Gilmour Hendrix said:
Synack said:
Pin said:
then transfer from loop to agar


How, precisely? Keep in mind the goal here is to avoid streaking the plate.


I was told the opposite. I was told when germinating spores to streak the plate.


It's hard to "keep state" when a thread has this many unrelated posts/day, but this particular "sub-thread" goes back to https://www.shroomery.org/forums/showflat.php/Number/29335106#29335106 wherein beef suggests that streaking just spreads contam around the plate and is unnecessary, and that he was going to remove streaking from the HHG. I'm testing to see if that approach reduces the contams on these plates.


--------------------
:blackspy:00010010
"When a long train of abuses and usurpations, pursuing invariably the same Object evinces a design to reduce them under absolute Despotism, it is their right, it is their duty, to throw off such Government, and to provide new Guards for their future security."

The Hitchhiker's Guide to the Shroomery <-- This is the way.



Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: toadking]
    #29336559 -

toadking said:
Making a Jar of LC, I know its a less positively looked on tek but I’m doing 500 ML water 50 Ml corn syrup and 1 gram of peptone with injection port lids, would 2 ccs be enough from my starter culture or should i do 5? possibly even 10?!

Edit: I’m also planning to use the rest on my WBF jars, I have 4 quarts sterilized but only one syringe so I would rather make culture first. Can I keep sterilized jars stored for a bit so my culture develops? Or would I have to sterilize new grain


Please help:confused:


--------------------

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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