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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix] * 1
    #29335972 -

Gilmour Hendrix said:
Define "working"




--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29335978 -

Kloakk said:
Gilmour Hendrix said:
Define "working"





I definitely feel like a punk with this foot in my ass!


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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix]
    #29335981 -

what's a "isolated" spore syringe ?

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Re: Ask Quick Questions, Get Quick Answers [Re: divineverdance] * 1
    #29335985 -

Marketing gimmick


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29335990 -

I tested my LC after putting 2ml into a jar. The plates grew bacteria but the jar is growing mycelium with no sign of bacteria. I have had bad luck with this mushroom before and it is most likely contaminated even though it is growing in the jar. So do I let it colonize and then put a colonized grain on a plate to try to isolate the mycelium from the bacteria, or do I just start over from spores to agar plates?

Thanks!

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Re: Ask Quick Questions, Get Quick Answers [Re: capsap]
    #29336006 -

I would go with spores unless it's something you really want to save, or do both for the experience points.

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Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
    #29336010 -

Aren't cabins more for bacteria than mold?


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: divineverdance] * 3
    #29336012 -

Grey market LC jargon


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∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 1
    #29336032 -

Gilmour Hendrix said:
I definitely feel like a punk with this foot in my ass!


Then it's working :rockon:

PuffM4gicDr4gon said:
Aren't cabins more for bacteria than mold?


Yes, mold will crawl out :uhoh:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: capsap]
    #29336106 -

capsap said:
I tested my LC after putting 2ml into a jar. The plates grew bacteria but the jar is growing mycelium with no sign of bacteria. I have had bad luck with this mushroom before and it is most likely contaminated even though it is growing in the jar. So do I let it colonize and then put a colonized grain on a plate to try to isolate the mycelium from the bacteria, or do I just start over from spores to agar plates?

Thanks!


Post pictures of the plates and the jar. Maybe someone will see something that can help.

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Re: Ask Quick Questions, Get Quick Answers [Re: The Tao]
    #29336119 -

jumping from spores into LC is kindof a disaster.  really need to master clean plates first.


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The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: el53]
    #29336131 -

el53 said:
So how long after you built your PC was it before you found out you needed to close the pressure release valve?


Yes, I home built my All American 941 PC... DUH


This is agar, still warm, not LC. Do I need to do something different to avoid sediment? Or is it ok to pour plates with it as is?


--------------------
What's the hardest part of cultivating shrooms?
As Tom Petty said... "the wa-a-iting is the hardest part"

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Re: Ask Quick Questions, Get Quick Answers [Re: markh]
    #29336137 -

Whats your recipe and for how long did you PC it?

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Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
    #29336142 -

ansr said:
Whats your recipe and for how long did you PC it?


It is a premix agar/lme powder. Instructions were, 50 grams to one litre of water. The bottle only holds half a litre so i used 25 grams, added 1/2 gram of peptides.
PC'd at 15 psi for 30 minutes.


--------------------
What's the hardest part of cultivating shrooms?
As Tom Petty said... "the wa-a-iting is the hardest part"

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: markh]
    #29336149 -

Clear plates arent neccesary.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: markh]
    #29336150 -

30 minutes might be enough but do 45 next time. Sediments isn't really a problem as long as it's properly sterilized.
I use a magnetic stirrer before and after PC while it cools down.

Check this: https://www.shroomery.org/forums/showflat.php/Number/28188696


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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29336151 -

tryptkaloids said:
Clear plates arent neccesary.


Thanks. just wasn't sure if the sediment would cause problems or not.


--------------------
What's the hardest part of cultivating shrooms?
As Tom Petty said... "the wa-a-iting is the hardest part"

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: ansr]
    #29336152 -

ansr said:
30 minutes might be enough but do 45 next time. Sediments isn't really a problem as long as it's properly sterilized.
I use a magnetic stirrer before and after PC while it cools down.

Check this: https://www.shroomery.org/forums/showflat.php/Number/28188696




thank you sir. Wish I had seen that video before I started. LOL


--------------------
What's the hardest part of cultivating shrooms?
As Tom Petty said... "the wa-a-iting is the hardest part"

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: markh]
    #29336153 -

markh said:
el53 said:
So how long after you built your PC was it before you found out you needed to close the pressure release valve?


Yes, I home built my All American 941 PC... DUH


This is agar, still warm, not LC. Do I need to do something different to avoid sediment? Or is it ok to pour plates with it as is?


you know, i used to get this same shit.  same recipe i always used.  stipe and everyone gave me advice and different methods and the thing that fixed it was,

i moved.  i think its the water. chelation. perhaps high levels of mag or potassium, or calcium.  most municipal sources put it in the water for a variety of reasons, namely corrosion control.

who knows what the difference is now but it went away.

recon you could try using distilled water? id be interested to see if that helps.  if it does, id say we have our answer. it not, fuck i dont know.  never could figure it out.


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: markh]
    #29336156 -

markh said:
ansr said:
Whats your recipe and for how long did you PC it?


It is a premix agar/lme powder. Instructions were, 50 grams to one litre of water. The bottle only holds half a litre so i used 25 grams, added 1/2 gram of peptides.
PC'd at 15 psi for 30 minutes.



let me verify your recipe bc dont get what youre saying.

2% recipe at 1000ml = 20g of agar, 20g lme

500ml = 10g of each
i dont care about the peptides but if understand right your recipe is 2.25% yeah?  if the mix is 50/50 2% should be 20g for 1liter and 10g for a half liter.

make sense?

not that i think this is your problem. but i do think 2% should be max.  all the cool kids are going 1.5% these days.  not me tho. im not cool.


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

Edited by BeefExtreme (09/06/25 10:37 AM)

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Shop: MagicBag.co All-In-One Bags That Don't Suck   Sporeworks.EU Spores for European Microscopy   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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