callmehippie said:

I do the streaking and probaly I feel like most around here do it  for the reason Roscoe said exactly. But I don't do all the multiple flaming and stuff like that. I just simply do one zigzag down the center of the plate just to spread out what's clean from not clean just a tiny bit.

This way it's less complicated. You only lift the lid once and torch once and it's noob friendly.

Like this.

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Uhh, hold the damn phone, my plates from streaking a spore syringe or spore print look nothing like these glorious things. Mine look like I stir fried shit and sprayed it onto the plate with a mister.

Three examples, all done in front of a hood with sufficiently good sterile technique that I've piles of clean plates (quite a few of which are pictured above in this thread). Fresh loop for each plate, flamed, cooled in front of the hood, then (admittedly messily) used to streak.

First two are from a vendor spore syringe, which I shook like I had parkinsons, then dripped from the dispensing tip onto the edge of each plate, and then streaked it across the plates in one shot. I don't remember if I changed loops between plates for these, or flamed between them...



The last is from a spore print sent to me in the mail. In front of the hood, opened it, used a scalpel fresh from the sterile packaging to scrape the foil a couple times towards the edge of a plate until I saw some black bits fall, then [again, messily] streaked it around with a fresh loop.



I already got a couple transfers off the first plate that just started growing on the new plates. Looks like a dodged all the contam but needs more time to really know. But... I want streak plates that look like yours. What on earth are you doing differently that I can replicate??

nektar61 said:

Like adding Peptone to agar. There are a lot of old posts here talking about doing that. But peptone is food for bacteria, not needed for shrooms, and increases the chance of bacteria out competing shroom mycelium.

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callmehippie said:

Also lots of us around here are on the Spectrum in one way or another. And long detailed explanations kind of comes with the territory.

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Synack said:

callmehippie said:

I do the streaking and probaly I feel like most around here do it  for the reason Roscoe said exactly. But I don't do all the multiple flaming and stuff like that. I just simply do one zigzag down the center of the plate just to spread out what's clean from not clean just a tiny bit.

This way it's less complicated. You only lift the lid once and torch once and it's noob friendly.

Like this.

---

Uhh, hold the damn phone, my plates from streaking a spore syringe or spore print look nothing like these glorious things. Mine look like I stir fried shit and sprayed it onto the plate with a mister.

Three examples, all done in front of a hood with sufficiently good sterile technique that I've piles of clean plates (quite a few of which are pictured above in this thread). Fresh loop for each plate, flamed, cooled in front of the hood, then (admittedly messily) used to streak.

First two are from a vendor spore syringe, which I shook like I had parkinsons, then dripped from the dispensing tip onto the edge of each plate, and then streaked it across the plates in one shot. I don't remember if I changed loops between plates for these, or flamed between them...



The last is from a spore print sent to me in the mail. In front of the hood, opened it, used a scalpel fresh from the sterile packaging to scrape the foil a couple times towards the edge of a plate until I saw some black bits fall, then [again, messily] streaked it around with a fresh loop.



I already got a couple transfers off the first plate that just started growing on the new plates. Looks like a dodged all the contam but needs more time to really know. But... I want streak plates that look like yours. What on earth are you doing differently that I can replicate??

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You wanna take this one beef?
Jk.

It could just be those cultures aren't that clean to begin with.

For wedges.

Flame loop. Dip loop into receiving  plate to cool.

Use loop to scoop some agar.

Then streak. I zig zag don the center. Giving my some some room from the edges of the plate. 

Or I somtimes i also use a sterile swab. For smashed agar recived In the mail. Just dip and streak.

For spores

Flame Loop. Dip in receiving dish to cool. Scrape print. Streak in the same fashion.

I also use swabs. scrape  print and stresk. I've never had any issue doing this dry.

For MSS, LC, or LI.

Flame loop. Cool loop.  drop a small amount of the liquid from syringe onto the loop. Then streak.

Or dip swab/loop  and streak.  Ex. I recently used a swab/loop and dipped it in a cryovile and then streaked the plate with it.

The cleaner you're inoculant the better your chances. I believe in you


Good luck.
🤖




Ps. I sounded like a robot when I took out all my personality from this post to make it short and to the point.. Lol.  Its still long.

koma23 said:

Fellas, what's causing this? I have it on multiple grows, different culture's ... To high humidity?

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OLDDAD77 said:

koma23 said:

Fellas, what's causing this? I have it on multiple grows, different culture's ... To high humidity?

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Mushrooms do weird things, but typically split caps are a sign of less than ideal humidity. Other than a bit of dialing in on the tubs it’s really nothing to worry about.

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Synack said:

callmehippie said:

I do the streaking and probaly I feel like most around here do it  for the reason Roscoe said exactly. But I don't do all the multiple flaming and stuff like that. I just simply do one zigzag down the center of the plate just to spread out what's clean from not clean just a tiny bit.

This way it's less complicated. You only lift the lid once and torch once and it's noob friendly.

Like this.

---

Uhh, hold the damn phone, my plates from streaking a spore syringe or spore print look nothing like these glorious things. Mine look like I stir fried shit and sprayed it onto the plate with a mister.

Three examples, all done in front of a hood with sufficiently good sterile technique that I've piles of clean plates (quite a few of which are pictured above in this thread). Fresh loop for each plate, flamed, cooled in front of the hood, then (admittedly messily) used to streak.

First two are from a vendor spore syringe, which I shook like I had parkinsons, then dripped from the dispensing tip onto the edge of each plate, and then streaked it across the plates in one shot. I don't remember if I changed loops between plates for these, or flamed between them...



The last is from a spore print sent to me in the mail. In front of the hood, opened it, used a scalpel fresh from the sterile packaging to scrape the foil a couple times towards the edge of a plate until I saw some black bits fall, then [again, messily] streaked it around with a fresh loop.



I already got a couple transfers off the first plate that just started growing on the new plates. Looks like a dodged all the contam but needs more time to really know. But... I want streak plates that look like yours. What on earth are you doing differently that I can replicate??

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swankyorclord said:

Curious about everyone’s thoughts on brown rice for spawn grain. Im in the middle of my first grow (pins are coming up!), and used brown rice as my grain of choice (pressure cooked jars, not that uncle Ben’s bullshit). However, as I’ve been doing more research, it seems like a small minority of cultivators here use brown rice. Is it truly inferior to a grain like rye or millet?

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