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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] * 1
    #29333894 -

I'll try these different methods of streaking and the cabin to see what works best. I'll grab these plates that contamed and work them a little bit to see what comes of it. Thanks for the help, guys!


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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix]
    #29333903 -

If i’m making a jar of LC and have my mixture in my jar and everything ready to go except injecting how many CCs do i need to inject? Would 2 be enough?


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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix]
    #29333907 -

catnip40 said:
Never heard of anyone going this route but you can buy sterile water at tractor supply or similar stores as well as like  sterile dextrose i.v. solution at pharmacies

...but at the end of the day if u don't have a PC u should probably just stick to pf tek



I use an 8qt insta pot for my grains and agar.

I have week old plates still sitting in my closet. No contaim.

Of all the plates I’ve done so far I was confident in being clean cultures and putting them to grain I’m getting solid results.

I have one PE7 tub not growing but still learning agar and wasn’t confident I did that one right.

Just need longer time and to bleed out all the air and fill with steam so far.

Usually with my rye berry, I set it for 180 minutes at bed time and go to bed and wake up and take em out.


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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs] * 1
    #29333939 -

Splittinghairs said:
catnip40 said:
Never heard of anyone going this route but you can buy sterile water at tractor supply or similar stores as well as like  sterile dextrose i.v. solution at pharmacies

...but at the end of the day if u don't have a PC u should probably just stick to pf tek



I use an 8qt insta pot for my grains and agar.

I have week old plates still sitting in my closet. No contaim.

Of all the plates I’ve done so far I was confident in being clean cultures and putting them to grain I’m getting solid results.

I have one PE7 tub not growing but still learning agar and wasn’t confident I did that one right.

Just need longer time and to bleed out all the air and fill with steam so far.

Usually with my rye berry, I set it for 180 minutes at bed time and go to bed and wake up and take em out.


I use an instant pot as well and it kicks ass for agar! I have had contam issues with most cereal grains but since I've switched to brown rice I haven't looked back

Also you totally can make LI in an instant pot. Just double the recommended times at 15psi.


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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson] * 2
    #29333944 -

Absolutely different strokes and I remember reading that post. I did a few rotations in microbiology years ago and the streak, rotate and flame was the  way it was done in that lab. Another lab might have done it differently.
Knowledge is good.

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Re: Ask Quick Questions, Get Quick Answers [Re: tossed_salad]
    #29333945 -

The brown rice when I did it was coming out crappy. One reason I switched to rye berry.

I use the super simple 10/10 rye berry for my rye

https://www.shroomery.org/forums/showflat.php/Number/25047342


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Re: Ask Quick Questions, Get Quick Answers [Re: toadking] * 4
    #29333949 -

Dont inoculate lc via syringe. Verified clean agar culture is the only lc innoculant i can recommend in good faith.


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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 2
    #29333990 -

tryptkaloids said:
Dont inoculate lc via syringe. Verified clean agar culture is the only lc innoculant i can recommend in good faith.


Agar is love. And it’s such an easy step to set you up for success.


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Re: Ask Quick Questions, Get Quick Answers [Re: toadking]
    #29333992 -

toadking said:
If i’m making a jar of LC and have my mixture in my jar and everything ready to go except injecting how many CCs do i need to inject? Would 2 be enough?


youre talking a spore syringe i imagine?

shake that fucker real good and just a few drops maybe a quarter cc at the most.

its not recommended by any means to go this route as its way more likely to get contaminated than an agar culture but truth be told, this is how i did it countless times many years ago with success.

...until i stopped having success

good luck!

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Re: Ask Quick Questions, Get Quick Answers [Re: sloug] * 1
    #29334097 -

These are my first Albino’s I’ve gotten to fruit. They are ACC. What am I looking at here as far as the grow health and specimen quality and how do I know when these are ready?




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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112]
    #29334106 -

is that what i thnk it is in the top right corner of that pic...

:death:

ive never grown those but they look great from here otherwise. id reckon once the caps kinda stop opening up its time to pluck. or however long u mind havin trich bloom out lol

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Re: Ask Quick Questions, Get Quick Answers [Re: sloug]
    #29334109 -

sloug said:
is that what i thnk it is in the top right corner of that pic...

:death:



Ahh motherfucker. I didn’t even see that. I’ll have to check first thing in the morning and if it is, I’ll just harvest it all, clone a few, put them in the dehydrator and dump the tub. Fucknuggets!!!!

:cryaboutit:

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112]
    #29334117 -

yea sorry bub, im like 99.9% sure its the green goblin. u can absolutely wait till they mature even if the trich is spreading tho. but im a nasty bitch that way. just makes cleanup a mess. ill usually dump it in a trash bag outside and rinse the rest out in the tub or hose depending on what time a year it is

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112]
    #29334119 -

I have been trying to experiment with agar using BOD’s tek and have been having issues with exploding Pyrex. The first time was my fault, I had it in the microwave for about 6 minutes total, shaking it after 1:30 intervals. I had the cap loose, and figured I would get it most of the way in the microwave and then go to a water bath. I closed the lid to take my Pyrex from the microwave to the stove and put the bottle in the water bath in my pressure cooker and I forgot to loosen the lid again and it instantly exploded, shot glass all over, hit my closed eye lid. I felt humbled and took it as a lesson to be more careful.
I was trying to warm up some agar from my fridge today and read something like, don’t put it in for 8 minutes at a time, break up into small intervals. I unscrewed my Pyrex about 3/4 of a turn and heated for 2 mins. Shook it up and put in for 1:30. 1 minute in, it explodes all over the microwave. I assume I needed to loosen the top a lot more but was curious what those with more experience thought.

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Re: Ask Quick Questions, Get Quick Answers [Re: BlueNicie] * 1
    #29334126 -

pyrex media bottle?  hmm someone told me pyrex isnt what it once was. as in, not always actual borosilicate.  it really shouldnt break like that.


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Re: Ask Quick Questions, Get Quick Answers [Re: Henry Swanson]
    #29334128 -

Henry Swanson said:
Not a bad idea, when you put the culture on your plate place it off to the side and then follow this pattern with your loop. Try not to put too much liquid on the plate.



A lot of good info and tips in this thread.
c10's Agar Guide + Tips & Tricks


I've seen this a bunch of times, but never understood--why keep re-flaming the loop when you keep running it across the same plate with the same spores? What are you trying to sterilize away at that point?


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Re: Ask Quick Questions, Get Quick Answers [Re: Synack]
    #29334138 -

It’s dilution streaking, by flaming the loop between streaks you’re picking up fewer organisms, spores, ect.. each time. Then watching for small colonies of clean myc to grow and transferring away as soon as possible.

Edited by Henry Swanson (09/04/25 12:07 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: BeefExtreme]
    #29334145 -

BeefSupremeJr said:
pyrex media bottle?  hmm someone told me pyrex isnt what it once was. as in, not always actual borosilicate.  it really shouldnt break like that.


The Corning brand media bottles are legit borosilicate. Should say "Made in Germany" on them and are the same ones available here: https://www.amazon.com/dp/B07HM9CKR6

That said, borosilicate isn't indestructible. A younger, dumber me threw some leftover lasagna in an old pyrex baking dish from a cooler straight over some very hot coals on a weber kettle at a tailgate.. it didn't so much explode as.. crack violently after about 5 minutes.

Straight from the horse's mouth: https://certs-ecatalog.corning.com/life-sciences/product-descriptions/1395-500.pdf  It's only rated to 125C and so if it contacts the bottom (or the side close to the bottom) of a dry wall of the PC.. I imagine it might go pop...


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:blackspy:00010010
"When a long train of abuses and usurpations, pursuing invariably the same Object evinces a design to reduce them under absolute Despotism, it is their right, it is their duty, to throw off such Government, and to provide new Guards for their future security."

The Hitchhiker's Guide to the Shroomery <-- This is the way.



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Re: Ask Quick Questions, Get Quick Answers [Re: Synack]
    #29334150 -

Thanks, I wasn’t sure if I was expecting too much from my jars or if I misunderstood how long to heat. I am getting a bit gun shy now, will have to order some more and will look for ones made in Germany. The first time I understand but 3 minutes in the microwave with the lid cracked seemed at odds with what Bid was saying and made me wonder if I just got some kind of knock off pyrex. Will try again, appreciate the insight!

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Re: Ask Quick Questions, Get Quick Answers [Re: BlueNicie]
    #29334154 -

BlueNicie said:
Thanks, I wasn’t sure if I was expecting too much from my jars or if I misunderstood how long to heat. I am getting a bit gun shy now, will have to order some more and will look for ones made in Germany. The first time I understand but 3 minutes in the microwave with the lid cracked seemed at odds with what Bid was saying and made me wonder if I just got some kind of knock off pyrex. Will try again, appreciate the insight!


Agree, 3m in the nuker should be child's play for those bottles. All I can think of (aside from a knockoff) is are you absolutely sure the lid was cracked (enough not to pressurize)? If it had any material on the threads it might have sealed better than you expected even if it wasn't tightened.


--------------------
:blackspy:00010010
"When a long train of abuses and usurpations, pursuing invariably the same Object evinces a design to reduce them under absolute Despotism, it is their right, it is their duty, to throw off such Government, and to provide new Guards for their future security."

The Hitchhiker's Guide to the Shroomery <-- This is the way.



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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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