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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Puffin]
    #29322276 -

Puffin said:
Kloakk said:
Puffin said:
What's my best bet for sterilizing the temperature probe of one of these?




How high does the temperature go? Put it in liquid and boil it for 15 minutes?


What sort of liquid are we talking? Water would not work as the 100°C boiling point is too low. But perhaps some other liquid with a higher boiling point?

Edit: Never mind, it only heats up to 98.9°C


No it's not, you don't need more than boiling temperature if it's liquid, nothing will live in my wort after I boil it. I have wort that's been sitting for years without anything changing the OG(original gravity).


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29322285 -

Where can I buy trusted LC syringes?


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Re: Ask Quick Questions, Get Quick Answers [Re: toadking]
    #29322287 -

This site has vendors.
Once you've unlocked the marketplace, I would suggest using that to get spores directly from users.

It's actually against the rules to send liquid culture to quite a few people currently because of the active ingredients in it.
Check the regulation map to see if you can even get liquid culture sent to you.


--------------------

The Shroomery Manual
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Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns]
    #29322288 -

RoscoeReturns said:
:whathesaid:

What are you trying to accomplish with this set up?


Holding the agar at just above setting temperature while I make tea and then add chloramphenicol. And generally to pour at the lowest possible temperature every time with no stress.


--------------------
Protoplast Fusion


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Re: Ask Quick Questions, Get Quick Answers [Re: Puffin] * 5
    #29322292 -

Puffin said:
RoscoeReturns said:
:whathesaid:

What are you trying to accomplish with this set up?


Holding the agar at just above setting temperature while I make tea and then add chloramphenicol. And generally to pour at the lowest possible temperature every time with no stress.


Then just set a container with water on the hot plate, put the agar bottle in the container and the probe in the container, no need to sterilize the probe at all :rockon:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29322296 -

Kloakk said:
Puffin said:
RoscoeReturns said:
:whathesaid:

What are you trying to accomplish with this set up?


Holding the agar at just above setting temperature while I make tea and then add chloramphenicol. And generally to pour at the lowest possible temperature every time with no stress.


Then just set a container with water on the hot plate, put the agar bottle in the container and the probe in the container, no need to sterilize the probe at all :rockon:


:mindblown:


--------------------
Protoplast Fusion


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Re: Ask Quick Questions, Get Quick Answers [Re: Puffin] * 1
    #29322301 -

Puffin said:
:mindblown:


:rofl:

That homebrewer mentality finally paying off :muppet:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


Edited by Kloakk (08/22/25 06:57 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie] * 1
    #29322303 -

callmehippie said:
I haven't ever used oats before. I've been all about wild bird seed and millet. I will be trying some oats or wheat real soon though. Because it's what I can find cheapest at my local feed store.

But yes definitely rinse. As boxtrot said grains are filthy especially grains coming from feed stores and stuff like that.

As far as any other advice. Some others will have to help you out on that one. And I'll pay attention to learn from it as well LOL


On grains, I made a choice to buy whole grain from a for human consumption source. Very clean but we will see how it works out.


--------------------
You can ignore the truth to your final breath. But trust me, it will meet you there to be ignored no longer.

The answer to your question is almost always 42
“The answer is always grow more shroom.” Mr Piggy
- Those two answers coexist peacefully.

Quick Sterilization Reference for Altitude

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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix] * 1
    #29322304 -

Gilmour Hendrix said:
Thanks guys!

Dala,
You're right about the 7 jar max. I didnt think of that. Is it possible to run jars on their side with unmodded lids?

Arky,
I'll keep an eye out for better grain. This stuff adds too much time because of the 2-3x rinse. I understand one rinse being necessary.

The first time I made jars, I used 3-hour Oat Prep. I might try that again so I can fit 10 jars next time.



It's okay to run modded lidded jars on their sides to make 10 in your pc.

Dala was pointing out if you happen to specifically be using a low prep tek like yoshies. 

Then a modded lid could run you into problems. Because you have to put some water in the jar with the grain. And if you loosen the lid and it not leak proof anymore that water can leak out and then mess up your water ratios.

If you're doing a tek that takes a little more prep involving soaking or steeping/simmering then your jars with the modded Lids are perfectly fine on their sidess.


Also if you're using the plastic ball leak proof or the white generic Walmart or Wholesale store Lids you might only be able to fit two jars turned on their sides on top instead of 3.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: 1NFIN8]
    #29322305 -

1NFIN8 said:
callmehippie said:
I haven't ever used oats before. I've been all about wild bird seed and millet. I will be trying some oats or wheat real soon though. Because it's what I can find cheapest at my local feed store.

But yes definitely rinse. As boxtrot said grains are filthy especially grains coming from feed stores and stuff like that.

As far as any other advice. Some others will have to help you out on that one. And I'll pay attention to learn from it as well LOL


On grains, I made a choice to buy whole grain from a for human consumption source. Very clean but we will see how it works out.



How much more do you pay for that tho? Just curious.


--------------------
:hippie::mushroom2:Take it easy. if it's easy. take it twice:mushroom2::mushroom2:
:sporedrop:The Hitchhiker's Guide to the Shroomery:sporedrop:

**A LINK TO EVERTHING**


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Re: Ask Quick Questions, Get Quick Answers [Re: callmehippie]
    #29322317 -

callmehippie said:
1NFIN8 said:
callmehippie said:
I haven't ever used oats before. I've been all about wild bird seed and millet. I will be trying some oats or wheat real soon though. Because it's what I can find cheapest at my local feed store.

But yes definitely rinse. As boxtrot said grains are filthy especially grains coming from feed stores and stuff like that.

As far as any other advice. Some others will have to help you out on that one. And I'll pay attention to learn from it as well LOL


On grains, I made a choice to buy whole grain from a for human consumption source. Very clean but we will see how it works out.


How much more do you pay for that tho? Just curious.


It’s expensive but I have small needs. 50 pounds organic hard wheat or rye for $80. I have no doubt non-organic berries but for human consumption would be cheaper.

This is what it looks like right out of the bag. It rinses clear as well.



--------------------
You can ignore the truth to your final breath. But trust me, it will meet you there to be ignored no longer.

The answer to your question is almost always 42
“The answer is always grow more shroom.” Mr Piggy
- Those two answers coexist peacefully.

Quick Sterilization Reference for Altitude

Edited by 1NFIN8 (08/22/25 07:09 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #29322326 -

Kloakk said:
Puffin said:
:mindblown:


:rofl:

That homebrewer mentality finally playing off :muppet:


Absolutely.

I'm curious though. When I brew, which I'm well overdue for at the moment admittedly, I pitch the yeast pretty much as soon as the wort is cooled. How do you have year plus old wort? Do you keep some for agar?


--------------------
Protoplast Fusion


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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix] * 3
    #29322327 -

Gilmour Hendrix said:
I'm going to pack 10 quart jars into my PC with unmodified plastic lids. I know I should leave the lids cracked but do I need to wrap the tops with foil? Gonna try Yoshi's low-prep grains this time around.


the foil serves two purposes. 
1 is it keeps your synthetic filters from getting wet, but you dont need that since youre doing unmod.

2 is that it keeps your lids clean until youre ready to work.  dont take the foil off till its either in front of your ffu or in your sab


--------------------
The Cultivation Confessional Booth - The OFFICIAL Cultivation Off Topic Discussion

✸-✸-✸

The Hitchhiker's Guide to the Shroomery

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Re: Ask Quick Questions, Get Quick Answers [Re: 1NFIN8] * 1
    #29322330 -

1NFIN8 said:
This tub was started a few weeks ago and this popped up in the past day. APE. Bad or good?




Btw on this - I dumped it but dug around to see what was up. The contam was surface only and I had a thick casing layer so I could have made some effort to save it with obviously questionable results, likely still a fail. That said, I had double the coir amount due to loopy math and the mycelium was nowhere near 10-20% after a month.

So, now that I’ve learned a ton, my fourth tub will be a liquid to agar to grain bottle attempt with TW. Learn, learn, and more learn. And even the failures are fun.


--------------------
You can ignore the truth to your final breath. But trust me, it will meet you there to be ignored no longer.

The answer to your question is almost always 42
“The answer is always grow more shroom.” Mr Piggy
- Those two answers coexist peacefully.

Quick Sterilization Reference for Altitude

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: 1NFIN8] * 2
    #29322334 -

It's only appearing on the surface there. It formed from that substrate. Which means it's contaminated.
It would start popping up more if you hadn't killed it.


--------------------

The Shroomery Manual
Wanna Play?
Dream Interpretation
GC’s Ayahuasca Recipe
Free Therapy

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Re: Ask Quick Questions, Get Quick Answers [Re: Puffin] * 1
    #29322344 -

Puffin said:
Absolutely.

I'm curious though. When I brew, which I'm well overdue for at the moment admittedly, I pitch the yeast pretty much as soon as the wort is cooled. How do you have year plus old wort? Do you keep some for agar?


Oh man don't get me started, or that's too late I guess :lol:

I make yeast starters, and with that I need wort to get them yeast multiplying right :naughty: So once in a while I'll take water and DME and make a 1.040 OG wort. Boil it and put it on bottles for when I need to make a starter. Well, after I started growing shrooms(10 months) I have brewed beer twice(it used to be twice a month) so now I have 9 liters of starter wort that I have not used. And they still smell nice and no carbonation or drop in SG.

And I buy my DME in bulk(25kg) so yeah I use the same DME when I make my agar plates and have more then enough to make plates :muppet:

Since you brew I just have to ask, what is your preferred beer style?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 3
    #29322347 -

Kloakk said:
Puffin said:
RoscoeReturns said:
:whathesaid:

What are you trying to accomplish with this set up?


Holding the agar at just above setting temperature while I make tea and then add chloramphenicol. And generally to pour at the lowest possible temperature every time with no stress.


Then just set a container with water on the hot plate, put the agar bottle in the container and the probe in the container, no need to sterilize the probe at all :rockon:


That’s a bingo! Hot water bath solves all the problems.


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Ask Quick Questions, Get Quick Answers [Re: RoscoeReturns] * 1
    #29322349 -

RoscoeReturns said:
That’s a bingo! Hot water bath solves all the problems.


:tipofthecap:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Gilmour Hendrix] * 1
    #29322375 -

Gilmour Hendrix said:
Another couple of questions before I get down to it.

How much diatemaceous earth is needed to preserve a 50 pound bag of grain?

I ended up with producers pride whole oats. To rinse or not to rinse, that is the question... no... really, guys... that's the question. 😁


I believe you said you were using Yoshi's No Prep tek, he explains how to do the rinse and preserve the water ratio in his instructions.

But first I would set up a fan, at the appropriate distance from the fan,pour the oats at a slow controlled speed - it will blow away lots of the muck off quickly. You will still need to do a water rinse, but it makes the water rinse easier.

Don't do the top three quarts. Do the seven vertical.

If this is your first run with Yoshi.. and you want a starting point...my ratio for a myco quart is
1.5 C. (180g) oats
1.7 C. (205g) water (distilled/R.O. if available)
When done, this may appear a little dry, but fewer burst grains, and I have never had a dryness problem, YMMV.

BTW, I don't use that brand of oats.

If you're using diatemaceous earth wear a mask. Always!


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes


Golden Teachers

Edited by Proposition 122 (08/22/25 07:51 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #29322407 -

Thank you.

By the way, I got "got" by an old lady on my PC. Was a local listing as a 23qt. I figured out when I was loading it earlier that it's only a 16qt. I had a new 23qt but shipped it back (cost me 13 bucks in shipping) because I found this one for 50. So now I have to buy a 23qt again. Damn it, man. Always something. Maybe I'll sell this one in the future.

I think I will store my grains in buckets and forego the DE. Any reason to keep DE around for cult?


--------------------



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Shop: Original Sensible Seeds Autoflowering Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   Myyco.com APE Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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