Synack said:

cronicr said:

Naw not even a bit lol, clean plates taste great.

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MEA plates are just sweetened agar, so I expect it would taste a little like shitty jello. However, I am less optimistic about the taste of the myc. The fruits taste like shit, why would the myc be any better?

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Dry fruit tastes like shit , cubes and most magic mushrooms are some of the best tasting mushrooms in the culinary world you could find
But the taste test stands true...it's either going to taste like mushrooms or ass with sugar( don't ask how I know or we got issues )

misterswisher said:

Puffin said:

The stage after this is to generate auxotrohpic strains for each monokaryotic culture using UV mutagenesis. Auxotrophs are mutants that can't create a certain nutrient so rely on it being in their environment to survive. The idea is that each parent has a different auxotrophism, then after protoplast fusion (forced mating essentially), when the resulting cultures are cultured on minimal media, the only ones that will survive and grow are hybrids, with the working gene from the other parent for to fix their auxotrophism. Self mated parents do not survive do not survive, so it's a way to find 'the needle in the haystack' when a hybridisation does occur.

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I skipped over this. How do you plan to create auxotophic strains ? I’m a poorly educated amateur so maybe this is easily achievable but that sounds like years of work in itself is it not?

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dopesauce said:

First run og HG plates. Milo’s pda

Gonna try some sextoring.
Was thinking of putting 4 sectors from each player on one dish, to compare growth rates.

What should I do with the rest of the plate? Was thinking of just putting it to grain, then just clone the best fruits in order to start isolating from results vs conjecture from looking at the plates.

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nektar61 said:

Puffin said:

My ambition is to create novel hybrids using protoplast fusion.

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Is this possible without a high end lab?
How do you plan to remove the cell wall, for instance?

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cronicr said:

But I'm half buzzed , am I tripping or does the growth itself look suspect?

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PuffM4gicDr4gon said:

cronicr said:

But I'm half buzzed , am I tripping or does the growth itself look suspect?

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I recently got into a huge argument on reddit about similar growth being contam (not the halo). To me it looks powdery and unorganized. The argument centered on whether or not that was tomentose growth. If anyone has any thoughts, I'd appreciate the input.

If it were my plate, I'd probably toss growth like that. Note for OP, this is only what I would do, not me telling you to toss the plate.

I was burned many times when I started growing by running less than perfect plates. I'm much pickier now.

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BeefSupremeJr said:

halos and concentric rings are from light cycles..  you got your light on a timer?  this plate looks like it was in the light for a week and then a long dark cycle.

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dopesauce said:

Gonna try some sextoring.
Was thinking of putting 4 sectors from each player on one dish, to compare growth rates.

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ThurmanMurman said:

BeefSupremeJr said:

halos and concentric rings are from light cycles..  you got your light on a timer?  this plate looks like it was in the light for a week and then a long dark cycle.

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Well I'll be damned. My ATL7 plates make this badass ripple affect of evenly spaced rings and 12hr light cycles would make too much sense.

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BeefSupremeJr said:

halos and concentric rings are from light cycles..  you got your light on a timer?  this plate looks like it was in the light for a week and then a long dark cycle.

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catnip40 said:

I bet ur sector is homokaryotic got the demeanor of a mf that eat corn the long way

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Kloakk said:

ThurmanMurman said:

BeefSupremeJr said:

halos and concentric rings are from light cycles..  you got your light on a timer?  this plate looks like it was in the light for a week and then a long dark cycle.

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Well I'll be damned. My ATL7 plates make this badass ripple affect of evenly spaced rings and 12hr light cycles would make too much sense.

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I get the zen garden rings when I have temperature fluctuations.

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That'd make sense too. Now I wanna keep the plates in the dark and see which it is.



Here's the ATL7. Just sharing bc I thought it looked cool, and I'm a simple man who's easily entertained.

cronicr said:

PuffM4gicDr4gon said:

cronicr said:

But I'm half buzzed , am I tripping or does the growth itself look suspect?

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I recently got into a huge argument on reddit about similar growth being contam (not the halo). To me it looks powdery and unorganized. The argument centered on whether or not that was tomentose growth. If anyone has any thoughts, I'd appreciate the input.

If it were my plate, I'd probably toss growth like that. Note for OP, this is only what I would do, not me telling you to toss the plate.

I was burned many times when I started growing by running less than perfect plates. I'm much pickier now.

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Ok 👌 am beyond half buzzed now....fully fucked but I agree forget the halo wtf is with the plate itself lol

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