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Harpo67
Stranger

Registered: 05/03/25
Posts: 122
Loc: United States of America

Re: Ask Quick Questions, Get Quick Answers

[Re: Mr Piggy]
#29290073 - 07/21/25 02:33 PM (1 year, 3 months ago)

Mr Piggy said:

Harpo67 said:

Here's some T2 plates of GT. I started with spores. Is the translucent growth normal or are all these contaminated?
Can I use any of these for inoculation of grain?

I'm still trying to understand how to use agar and what I'm looking at.

Transfer from 10 oclock on the bottom right plate, and five oclock on the bottom left plate. See how that grows out.

I really like to see uniform growth before transferring to grain.

Thanks to you and Tree Frog. I will do those transfers.

Still would like feedback on my other question. Is the translucent growth normal or is that contamination?

--------------------
Is not what we see or seem but a dream within a dream? -Edgar Allan Poe

:mushroom2:

:mushroom2:

The Hitchhikers Guide to the Shroomery :mushroom2:

:mushroom2:

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Splittinghairs

Registered: 02/17/25
Posts: 1,343

Re: Ask Quick Questions, Get Quick Answers

[Re: Harpo67]
#29290080 - 07/21/25 02:37 PM (1 year, 3 months ago)

Ok. Information all over the place about foil.

If I inoculate a grain quart jar, no filter, no verm layer, lid upside down quarter turn back off…….

Do I remove the foil after inoculation ?

--------------------
Favorite Teks

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nocap
for real

Registered: 09/30/24
Posts: 1,573
Last seen: 10 months, 23 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Harpo67]
#29290085 - 07/21/25 02:38 PM (1 year, 3 months ago)

Is tough to tell for sure with that lighting. Some look like fuzzy mycelium, but others are more questionable. Is there matte biofilm in those areas? To where the agar has lost its gloss shine on the surface. Maybe some photo with top/side lighting and less/no backlighting would be more “illuminating”.

--------------------
The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
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nocap
for real

Registered: 09/30/24
Posts: 1,573
Last seen: 10 months, 23 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Splittinghairs]

3
#29290086 - 07/21/25 02:39 PM (1 year, 3 months ago)

Ok to remove foil 👍

Edit - This was post 666 for me
:sundevil:

:sundevil:

--------------------
The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

Edited by nocap (07/21/25 02:41 PM)

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Nichrome
-675 kPa

Registered: 12/17/18
Posts: 8,963

Re: Ask Quick Questions, Get Quick Answers

[Re: Harpo67]

1
#29290088 - 07/21/25 02:41 PM (1 year, 3 months ago)

The colony is just favoring one group over another. Often when you transfer that stuff to it's own dish it will get aggressive...

Long story short it is still your target species and not contamination. Stuff that is taking a back seat to the plans of the colony as a whole.

--------------------
Question everything, it's very responsive.

Culture Storage

Edited by Nichrome (07/21/25 02:43 PM)

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Mr Piggy
Swine Flew

Registered: 09/29/11
Posts: 16,768
Loc: bioilliminicent©️

Re: Ask Quick Questions, Get Quick Answers

[Re: Harpo67]
#29290091 - 07/21/25 02:44 PM (1 year, 3 months ago)

Harpo67 said:

Mr Piggy said:

Harpo67 said:

Here's some T2 plates of GT. I started with spores. Is the translucent growth normal or are all these contaminated?
Can I use any of these for inoculation of grain?

I'm still trying to understand how to use agar and what I'm looking at.

Transfer from 10 oclock on the bottom right plate, and five oclock on the bottom left plate. See how that grows out.

I really like to see uniform growth before transferring to grain.

Thanks to you and Tree Frog. I will do those transfers.

Still would like feedback on my other question. Is the translucent growth normal or is that contamination?

I do not see any translucent growth :shrug:

:shrug:

--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿

New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery

Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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mushboy
modboy

Registered: 04/24/05
Posts: 41,022
Loc: the woods

Re: Ask Quick Questions, Get Quick Answers

5
#29290120 - 07/21/25 03:09 PM (1 year, 3 months ago)

:whathesaid:

its just different morphology or whatever.

id probably do another transfer cause i like my shit to be symmetrical.

--------------------
:hypnotoad:

:hypnotoad:

Making Liquid Culture : Go insane with LC
Mushboy likes these teks : Making shroom tea
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Harpo67
Stranger

Registered: 05/03/25
Posts: 122
Loc: United States of America

Re: Ask Quick Questions, Get Quick Answers

[Re: nocap]
#29290260 - 07/21/25 05:17 PM (1 year, 3 months ago)

nocap said:

Is tough to tell for sure with that lighting. Some look like fuzzy mycelium, but others are more questionable. Is there matte biofilm in those areas? To where the agar has lost its gloss shine on the surface. Maybe some photo with top/side lighting and less/no backlighting would be more “illuminating”.

Are these pics better? Seems like fuzzy mycellium. But I'm not yet an expert.

--------------------
Is not what we see or seem but a dream within a dream? -Edgar Allan Poe

:mushroom2:

:mushroom2:

The Hitchhikers Guide to the Shroomery :mushroom2:

:mushroom2:

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nocap
for real

Registered: 09/30/24
Posts: 1,573
Last seen: 10 months, 23 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Harpo67]
#29290285 - 07/21/25 05:39 PM (1 year, 3 months ago)

Those are great pics 👍
Looks like fuzzy mycelium to my untrained eye also

I would maybe take a transfer of that too. Just for fun.
:mitebecool:

:mitebecool:

--------------------
The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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Mr Piggy
Swine Flew

Registered: 09/29/11
Posts: 16,768
Loc: bioilliminicent©️

Re: Ask Quick Questions, Get Quick Answers

[Re: nocap]

1
#29290322 - 07/21/25 06:15 PM (1 year, 3 months ago)

It’s just tomentose myc, totally normal and it will also kick out fruits. Rhizo just looks real purdy on plates.

--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿

New to cultivation or the shroomery? Observe the hitchhiker's guide
The Hitchhiker's Guide to the Shroomery

Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Mub
Certified shitshow

Registered: 02/04/25
Posts: 166
Loc: OH
Last seen: 6 days, 19 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: Mr Piggy]
#29290411 - 07/21/25 07:28 PM (1 year, 3 months ago)

My second round of bins ever. Looks wet with some puddling around the edges but the middle area looks a little dry to me. Any advice?

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dinosaurs
Permanently Banned

Registered: 03/10/25
Posts: 2,115

Re: Ask Quick Questions, Get Quick Answers

[Re: Mub]
#29290420 - 07/21/25 07:39 PM (1 year, 3 months ago)

Mub said:

My second round of bins ever. Looks wet with some puddling around the edges but the middle area looks a little dry to me. Any advice?

if you dont use a liner, pooling on the edges can happen as condensate runs down the sides of the tub. you can mop it up with paper towel if it pools.

among others, this is a reason I'd advocate in favor of liners. with a liner in place, the water that runs down the sides ends up trapped between the tub and the liner instead of running down onto your substrate or between the tub and substrate.

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,576
Loc: Exact Center
Last seen: 20 hours, 12 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: dinosaurs]

1
#29290425 - 07/21/25 07:42 PM (1 year, 3 months ago)

Looks like youre about to have a killer pinset

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

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Mub
Certified shitshow

Registered: 02/04/25
Posts: 166
Loc: OH
Last seen: 6 days, 19 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]
#29290441 - 07/21/25 08:05 PM (1 year, 3 months ago)

Hell yea thanks, I hope so 🤞

I’m open to try a liner. I’ve enjoyed trying different things. What kind of liner is recommended.

Edited by Mub (07/21/25 08:05 PM)

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boxtrotalpha
rascal bandit mushroom slave

Registered: 06/17/24
Posts: 2,616
Loc: The rings of Saturn
Last seen: 10 months, 13 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Mub]
#29290443 - 07/21/25 08:13 PM (1 year, 3 months ago)

Grains were much to wet when I noccd it and now it's lookin kinda suspect. I'm not seeing any piss anywhere and this is 48 hours after a shake but I'd like some pro opinions. Toss? Top fruit? Shoebox at 1:1 or 1:2?

--------------------
Where to start?
Are your plates clean?

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Proposition 122
Bonehead

Registered: 11/14/22
Posts: 715
Loc: Colorado

Last seen: 9 months, 14 days

Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]
#29290446 - 07/21/25 08:17 PM (1 year, 3 months ago)

Conversion confusion :confused:

:confused:

I only have liquid LME, not powdered.
So does 1g powdered LME = 1g of liquid LME?

--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

Fruiting Cubes

Golden Teachers

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phishinphool
Stranger

Registered: 09/30/12
Posts: 35
Loc: United States
Last seen: 1 month, 2 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Proposition 122]
#29290450 - 07/21/25 08:23 PM (1 year, 3 months ago)

1st time using a ready to fruit bag (prior PF tek guy). Got one in the mail colonized on substrate. The "instrucitons" that came with it said to stand up the bag and that way it can breathe out of the white filter patch. NOwhere does it say I should cut the bag / make slits. Most videos Iv'e seen where people colonize bags themselves they always cut the bags to vent and allow fresh air exchange. Should I cut my bag to allow fresh air and mist as necc?

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,576
Loc: Exact Center
Last seen: 20 hours, 12 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: Proposition 122]
#29290455 - 07/21/25 08:26 PM (1 year, 3 months ago)

Proposition 122 said:

Conversion confusion
I only have liquid LME, not powdered.
So does 1g powdered LME = 1g of liquid LME?

Try it and get back to us. I assume its stronger bht ive never used it.

Worst that could happen is you make em too strong and you slow your culture down and make it tomentose

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

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boxtrotalpha
rascal bandit mushroom slave

Registered: 06/17/24
Posts: 2,616
Loc: The rings of Saturn
Last seen: 10 months, 13 days

Re: Ask Quick Questions, Get Quick Answers

[Re: phishinphool]
#29290456 - 07/21/25 08:26 PM (1 year, 3 months ago)

phishinphool said:

1st time using a ready to fruit bag (prior PF tek guy). Got one in the mail colonized on substrate. The "instrucitons" that came with it said to stand up the bag and that way it can breathe out of the white filter patch. NOwhere does it say I should cut the bag / make slits. Most videos Iv'e seen where people colonize bags themselves they always cut the bags to vent and allow fresh air exchange. Should I cut my bag to allow fresh air and mist as necc?

Ready to fruit bag? Like an all in one bag that's already fully colonized by mycelium?

--------------------
Where to start?
Are your plates clean?

Extras:

tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,576
Loc: Exact Center
Last seen: 20 hours, 12 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: phishinphool]
#29290457 - 07/21/25 08:28 PM (1 year, 3 months ago)

phishinphool said:

1st time using a ready to fruit bag (prior PF tek guy). Got one in the mail colonized on substrate. The "instrucitons" that came with it said to stand up the bag and that way it can breathe out of the white filter patch. NOwhere does it say I should cut the bag / make slits. Most videos Iv'e seen where people colonize bags themselves they always cut the bags to vent and allow fresh air exchange. Should I cut my bag to allow fresh air and mist as necc?

Ive never fruited from bags, but it depends on if you are opening the top of the bag or not. You dont want things drying out too fast but you do want the culture to breathe. 2-4 nail head sized holes melted at sub level should be perfect if the top is sealed.

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.

Extras:

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