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The Tao
Read more, post less.

Registered: 09/12/19
Posts: 3,935
Loc: Canada

Last seen: 1 hour, 5 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: PompNCircumstance]

7
#29287481 - 07/19/25 09:45 PM (1 year, 3 months ago)

RoscoeReturns said:

QQ Should I care about any of this now?

I’m with you in spirit but minus a sausage and I had to have a faux fire. I label my initial plates G for germination, I don’t know why. I consider this place as a melting pot of information and ideas. Lots of great work gets done here.

Quote:

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,612
Loc: Hollywoo

Last seen: 1 day, 14 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: The Tao]
#29287498 - 07/19/25 09:54 PM (1 year, 3 months ago)

The Tao said:

I’m with you in spirit but minus a sausage and I had to have a faux fire. I label my initial plates G for germination, I don’t know why. I consider this place as a melting pot of information and ideas. Lots of great work gets done here.

I like that photo. haha.
I think you're confusing successive plate transfers (Germ(ination) plate, T1, T2, T3, whree "T" is "transfer" with filial generations from selection (P, F1, F2, etc), which can be done without agar (and was by fhat until a while back)

Very common error, thus, "What's T1, T2, T3 etc. And what's F1, F2, F3?"

You've got it right though, first plate where you toss spores on is germination plate.

A T1 could be an F1 (or an Fx), but doesn't have to be. Two different processes.

--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (07/19/25 10:01 PM)

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RoscoeReturns
We’ve outstayed our welcome

Registered: 02/13/18
Posts: 3,129
Loc: State of Confusion
Last seen: 42 minutes, 8 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: The Tao]

10
#29287500 - 07/19/25 09:57 PM (1 year, 3 months ago)

Great night for a fire. I watched the bats feed before full dark. Now I’m just smelling like wood smoke and looking at stars. I don’t know what the fuck y’all are up to in here. Hope you have a good night.

The Tao said:

RoscoeReturns said:

QQ Should I care about any of this now?

I’m with you in spirit but minus a sausage and I had to have a faux fire. I label my initial plates G for germination, I don’t know why. I consider this place as a melting pot of information and ideas. Lots of great work gets done here.

Those are some damn fine pants.

--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?

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nocap
for real

Registered: 09/30/24
Posts: 1,573
Last seen: 10 months, 22 days

Re: Ask Quick Questions, Get Quick Answers

[Re: The Tao]
#29287508 - 07/19/25 10:04 PM (1 year, 3 months ago)

The Tao said:

not hotdog 🌭

--------------------
The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

Edited by nocap (07/19/25 10:04 PM)

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PuffM4gicDr4gon
Professional Coir Waster

Registered: 06/09/11
Posts: 2,854
Loc: The Land of Honnalee
Last seen: 5 minutes, 26 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: nocap]

5
#29287530 - 07/19/25 10:16 PM (1 year, 3 months ago)

I like to heat my wiener using a digital screen. Usually from friction.

--------------------
I've got skills, they don't know where to use me
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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,612
Loc: Hollywoo

Last seen: 1 day, 14 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: nocap]

2
#29287534 - 07/19/25 10:25 PM (1 year, 3 months ago)

nocap said:

The Tao said:

not hotdog 🌭

JIAN-YANG!!!!

--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Extras:

SexxLaws
BECK

Registered: 03/09/24
Posts: 30
Last seen: 3 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

[Re: PompNCircumstance]
#29287568 - 07/19/25 11:03 PM (1 year, 3 months ago)

I have a spore syringe from a friend and I want to put it on agar. Will it be good enough to just put a few drops on the agar plates, or should I use a loop and put the syringe into a clean shot glass to streak the plates?

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Arkyhog
Truth Seeker

Registered: 03/25/24
Posts: 832
Loc: Mushroom Kingdom

Last seen: 12 days, 4 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: SexxLaws]
#29287572 - 07/19/25 11:07 PM (1 year, 3 months ago)

SexxLaws said:

I have a spore syringe from a friend and I want to put it on agar. Will it be good enough to just put a few drops on the agar plates, or should I use a loop and put the syringe into a clean shot glass to streak the plates?

Why not do both?

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Nichrome
-675 kPa

Registered: 12/17/18
Posts: 8,963

Re: Ask Quick Questions, Get Quick Answers

6
#29287578 - 07/19/25 11:13 PM (1 year, 3 months ago)

You ok Nektar?

You're having a melt down about not a lot.

The thing is, in this world it doesn't seem to matter how smart you are, or what you know, or what you have, if you can't communicate in a way which will be received.

This thread was designed as a platform for questions and answers specifically and the guidelines were laid out pretty clearly. Confrontation, ego trips, superiority, or speaking negatively about other people for any reason is absolutely not in line with the theme of this thread, and I have reminded you of this multiple times. Enough times to where I was honestly relieved when you ignored me for the last half of a day, and not surprised when you un-ignored me and came into my thread on a rampage again. It is weird and I don't like it.

Anyways I'd like it if you took your superiority complex somewhere else so this thread can return to its intended roll. Or at least re-read the OP and check the baggage at the door, ya know, leave the dirty laundry in the laundry room...

--------------------
Question everything, it's very responsive.

Culture Storage

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SexxLaws
BECK

Registered: 03/09/24
Posts: 30
Last seen: 3 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Arkyhog]
#29287581 - 07/19/25 11:16 PM (1 year, 3 months ago)

I guess I just don't have the inoculation loop, but im wondering if that is how you should do it.

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Nichrome
-675 kPa

Registered: 12/17/18
Posts: 8,963

Re: Ask Quick Questions, Get Quick Answers

3
#29287587 - 07/19/25 11:18 PM (1 year, 3 months ago)

You don't need to do all that with the loop, especially if you don't have a lot of hours in the cockpit. You are just opening up more avenues for contamination to creep in. A few drips from a syringe on agar is fine and dandy.

--------------------
Question everything, it's very responsive.

Culture Storage

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SexxLaws
BECK

Registered: 03/09/24
Posts: 30
Last seen: 3 months, 28 days

Re: Ask Quick Questions, Get Quick Answers

[Re: Nichrome]
#29287598 - 07/19/25 11:32 PM (1 year, 3 months ago)

Thank you!

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,612
Loc: Hollywoo

Last seen: 1 day, 14 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: nektar61]
#29287629 - 07/20/25 12:06 AM (1 year, 3 months ago)

Nichrome said:

You ok Nektar?

You're having a melt down about not a lot.
....Anyways I'd like it if you took your superiority complex somewhere else so this thread can return to its intended roll.

I'm not having a meltdown, I'm calm. I'm answering questions.
And the last few replies of mine on here have been very calm, the one you quoted was even playing along with a pop culture reference that has nothing to do with this topic. I was trying to change the subject. You seem triggered, not me.

And I'm not being mean to anyone, just matter of fact.

But I'll hold back what I've been thinking and not saying. Having several people give me shit for using correct terms reminds me of the running joke in Idiocracy where the low-IQ people call the average-IQ person "FAG" because he speaks in complete sentences.

I'm going go back to having you, Nichrome, on ignore, like I did for over 2 years.

I only recently took you off ignore, and it was a mistake. If you want to know why I had you on ignore, it was for this totally insane post. I can't believe you said that acting like a serial killer is a good way to keep the cops from paying attention to you (I thought you were joking but you got mad at me for thinking that in subsequent posts, and then complained that I break up paragraphs instead of posting text blocks):

Quote:

Or you could just be so weird that anyone and everyone is mortified to even look at anything in your yard let alone want to go through your garbage.

Leaving hides and bones and other animal parts all around is a good start. Piles of moldy stuff helps too. Most people are afraid of fungus. People don't want to know what the fuck it is. Meter reader keeps his eyes front. Thinks you're a Jeff Dahmer or some shit.

---
OK, I'm done with this topic if you all are, and won't bring it up here today except to reply. But I'm still going to correct incorrect terms when someone uses them while trying to teach a new member here. Everyone should be doing that or we turn into Reddit.

--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (07/20/25 12:16 AM)

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nocap
for real

Registered: 09/30/24
Posts: 1,573
Last seen: 10 months, 22 days

Re: Ask Quick Questions, Get Quick Answers

1
#29287642 - 07/20/25 12:22 AM (1 year, 3 months ago)

If I wanted to save and dry a few grains of colonized spawn for future use, what would be an efficient way to accomplish that?

I was thinking that an empty jar or petri might do the trick or even cryovials. Although, I haven’t purchased those yet, because I’ve not convinced myself that I will actually use them.

--------------------
The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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dinosaurs
Permanently Banned

Registered: 03/10/25
Posts: 2,115

Re: Ask Quick Questions, Get Quick Answers

3
#29287645 - 07/20/25 12:25 AM (1 year, 3 months ago)

I edited out a shitty comment and replaced it with this bro that was hanging out with me on a stop on my cycling trip the other day.

Edited by dinosaurs (07/20/25 12:31 AM)

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nektar61
Just a smear in the shale

Registered: 07/04/20
Posts: 4,612
Loc: Hollywoo

Last seen: 1 day, 14 hours

Re: Ask Quick Questions, Get Quick Answers

[Re: nocap]

2
#29287649 - 07/20/25 12:29 AM (1 year, 3 months ago)

nocap said:

If I wanted to save and dry a few grains of colonized spawn for future use, what would be an efficient way to accomplish that?

I was thinking that an empty jar or petri might do the trick or even cryovials. Although, I haven’t purchased those yet, because I’ve not convinced myself that I will actually use them.

I didn't use a tek, I just put some in test tubes but you could use a Petri if you have those and don't want to buy something else. I kept these in the fridge, not the freezer. I should have let all of them get more colonized before putting them in but it worked. Bottom is them growing growing on plate after over a year after in the fridge.

--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Extras:

fahtster
Now With 33%More Faht

Registered: 06/17/06
Posts: 10,935

Re: Ask Quick Questions, Get Quick Answers

4
#29287654 - 07/20/25 12:34 AM (1 year, 3 months ago)

Ihe said dry grains. Put em in a swab pack. They’ll last for years. Cryovials work too.. I’d just be sure to wrap em

No need to refrigerate

You can just leave a small amount in the bottom of a spawn jar (obv with the lid back on.) but that takes a while. If you have a hood, you can put em in a Petri dish for a day and they’ll dry real fast

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tryptkaloids
Learner

Registered: 02/08/15
Posts: 16,576
Loc: Exact Center
Last seen: 17 hours, 57 minutes

Re: Ask Quick Questions, Get Quick Answers

[Re: fahtster]
#29287659 - 07/20/25 12:37 AM (1 year, 3 months ago)

Do you let the air out of your bags to colonize, or leave em filled up?

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
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ThurmanMurman
Sandwich Artist

Registered: 06/25/24
Posts: 3,640
Last seen: 3 hours, 41 minutes

Re: Ask Quick Questions, Get Quick Answers

2
#29287660 - 07/20/25 12:37 AM (1 year, 3 months ago)

fahtster said:

Ihe said dry grains. Put em in a swab pack. They’ll last for years. Cryovials work too.. I’d just be sure to wrap em

I just got cryovials and as I held them in my hand, I realized that I dont have any kind of plan for how to stand this tiny vial up with the cap off, place sample, and get the cap back on. All without spilling it or contaminating it. 🤣

Grains might be the way - thanks for mentioning it.🤙

--------------------

Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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dinosaurs
Permanently Banned

Registered: 03/10/25
Posts: 2,115

Re: Ask Quick Questions, Get Quick Answers

[Re: fahtster]
#29287661 - 07/20/25 12:37 AM (1 year, 3 months ago)

fahtster said:

Ihe said dry grains. Put em in a swab pack. They’ll last for years. Cryovials work too.. I’d just be sure to wrap em

No need to refrigerate

so just dehydrated grain spawn can be used as an inoculant? i assume the use-case is to agar to clean it up?

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Shop:

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