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catnip40
Puppet∴Master

Registered: 03/09/12
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Re: Ask Quick Questions, Get Quick Answers

[Re: nektar61]
#29281109 - 07/14/25 09:32 AM (1 year, 3 months ago)

I bet pf stropharia is faster

1:1 shoebox or pf cakes for breeding or pheno hunting ect

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Splittinghairs

Registered: 02/17/25
Posts: 1,343

Re: Ask Quick Questions, Get Quick Answers

1
#29281168 - 07/14/25 11:16 AM (1 year, 3 months ago)

So with inoculation loops would scraping spores into a small little bit of tap water help for agar?

Or just sterilize loop, drop water on loop, scrape print?

Or cool loop and dry scrape spores?

The journey has been fun so far. But got two prints I wanna do right and maximize yield

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mushboy
modboy

Registered: 04/24/05
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Loc: the woods

Re: Ask Quick Questions, Get Quick Answers

[Re: Splittinghairs]

2
#29281169 - 07/14/25 11:18 AM (1 year, 3 months ago)

you can do whatever is easiest for you just use sterile water.

i like keeping sterile water syringes handy for prints.

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Splittinghairs

Registered: 02/17/25
Posts: 1,343

Re: Ask Quick Questions, Get Quick Answers

[Re: mushboy]
#29281170 - 07/14/25 11:21 AM (1 year, 3 months ago)

Does it need to be sterile? Shoot.

Might need to just cool on agar and scrape. Don’t wanna blow the full print if it needs to be sterile.

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nocap
for real

Registered: 09/30/24
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Re: Ask Quick Questions, Get Quick Answers

[Re: Splittinghairs]

2
#29281173 - 07/14/25 11:22 AM (1 year, 3 months ago)

Definitely needs to be sterile.

I have had fine luck with dry loop scrapes. Give it a go.

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mushboy
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Re: Ask Quick Questions, Get Quick Answers

[Re: Splittinghairs]

4
#29281176 - 07/14/25 11:26 AM (1 year, 3 months ago)

Splittinghairs said:

Might need to just cool on agar and scrape. Don’t wanna blow the full print if it needs to be sterile.

you just need the tiniest scrapping of spores a single quarter sized print could make you about 50 syringes easily.

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Smellyhobbit
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Registered: 04/01/22
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Re: Ask Quick Questions, Get Quick Answers

[Re: nocap]

2
#29281177 - 07/14/25 11:26 AM (1 year, 3 months ago)

nocap said:

Definitely needs to be sterile.

I have had fine luck with dry loop scrapes. Give it a go.

For prints you don’t really NEED to add water. If you’re worried about hydration, poke loop into the agar during inoculation. Puts a few spores inside the agar. Usually I don’t have problems streaking even years old prints.

But as others have said, any water you use definitely needs to be sterile.

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GenesisCorrupted
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Registered: 08/01/23
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Re: Ask Quick Questions, Get Quick Answers

[Re: Smellyhobbit]

4
#29281179 - 07/14/25 11:28 AM (1 year, 3 months ago)

:goldenharvest:

Sterile you say?

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boxtrotalpha
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Re: Ask Quick Questions, Get Quick Answers

[Re: GenesisCorrupted]

4
#29281181 - 07/14/25 11:30 AM (1 year, 3 months ago)

Still gonna have to recook it. Need that twice baked piss

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yoosername
Lab Member

Registered: 03/29/13
Posts: 1,510
Loc: Terra Fracta

Re: Ask Quick Questions, Get Quick Answers

[Re: Splittinghairs]

6
#29281183 - 07/14/25 11:32 AM (1 year, 3 months ago)

I always end up with a few dishes with condensation on the lids. I occasionally use the condensation to wet the loop after dry scraping.

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nocap
for real

Registered: 09/30/24
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Re: Ask Quick Questions, Get Quick Answers

[Re: yoosername]

3
#29281190 - 07/14/25 11:41 AM (1 year, 3 months ago)

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boxtrotalpha
rascal bandit mushroom slave

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Re: Ask Quick Questions, Get Quick Answers

[Re: yoosername]

4
#29281196 - 07/14/25 11:47 AM (1 year, 3 months ago)

yoosername said:

I always end up with a few dishes with condensation on the lids. I occasionally use the condensation to wet the loop after dry scraping.

:shockhawk:

That's kinda fucking brilliant. Definitely adding this to my toolbox. Way easier than pressure cooking piss

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tryptkaloids
Learner

Registered: 02/08/15
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Re: Ask Quick Questions, Get Quick Answers

[Re: yoosername]

5
#29281213 - 07/14/25 12:14 PM (1 year, 3 months ago)

yoosername said:

I always end up with a few dishes with condensation on the lids. I occasionally use the condensation to wet the loop after dry scraping.

:whathesaid:

a lot of people hate condensation, but its great for germ plates

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Splittinghairs

Registered: 02/17/25
Posts: 1,343

Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]

1
#29281229 - 07/14/25 12:36 PM (1 year, 3 months ago)

Thanks for all the knowledge drop!

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boxtrotalpha
rascal bandit mushroom slave

Registered: 06/17/24
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Re: Ask Quick Questions, Get Quick Answers

[Re: Splittinghairs]
#29281398 - 07/14/25 04:35 PM (1 year, 3 months ago)

So I've got these unmodded 12 qt shoeboxes. Lotta condensation recycling happening right now which I'm assuming is fine since they're not fully colonized but is this gonna be a problem in the near future? If it is what's the plan, flip the lid and observe? Punch some holes?

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Vegeta

Registered: 04/22/24
Posts: 407
Last seen: 9 months, 16 days

Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]
#29281404 - 07/14/25 04:52 PM (1 year, 3 months ago)

tryptkaloids said:

yoosername said:

I always end up with a few dishes with condensation on the lids. I occasionally use the condensation to wet the loop after dry scraping.

a lot of people hate condensation, but its great for germ plates

Good tip. I always wrap a little stack of plates off the top asap after pouring to keep the condensation for germ plates

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Harpo67
Stranger

Registered: 05/03/25
Posts: 122
Loc: United States of America

Re: Ask Quick Questions, Get Quick Answers

[Re: tryptkaloids]

3
#29281409 - 07/14/25 04:59 PM (1 year, 3 months ago)

After a blob filled 500g first flush, this 2nd round of PE not looking too bad.

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Agar Wedge
Hairy Cheese

Registered: 12/25/24
Posts: 2,399
Loc: Pennsylvania
Last seen: 32 minutes, 15 seconds

Re: Ask Quick Questions, Get Quick Answers

[Re: Harpo67]
#29281429 - 07/14/25 05:13 PM (1 year, 3 months ago)

Anybody know if coir can be pastuerized using hydrated lime? I was thinking of adding lime water and soaking for 24 hours then bringing it too field capacity after pastuerization. Kinda like I do with the straw, minus the onion sack parts

https://www.shroomery.org/forums/showflat.php/Number/29212165

I have a couple large blocks from about 10 years ago that have been through 4 moves and not in sealed packages. Id hate to waste the stuff. Just wanted to see if anybody else has had luck in this department?
Thanks in advance for any input!

Edited by Agar Wedge (07/14/25 05:32 PM)

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mushboy
modboy

Registered: 04/24/05
Posts: 41,022
Loc: the woods

Re: Ask Quick Questions, Get Quick Answers

[Re: Agar Wedge]

4
#29281442 - 07/14/25 05:33 PM (1 year, 3 months ago)

why would it be wasted.

just add water and go about your business.

coir doesnt have an expiration date.

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Making Liquid Culture : Go insane with LC
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nocap
for real

Registered: 09/30/24
Posts: 1,573
Last seen: 10 months, 23 days

Re: Ask Quick Questions, Get Quick Answers

[Re: boxtrotalpha]
#29281443 - 07/14/25 05:34 PM (1 year, 3 months ago)

boxtrotalpha said:

So I've got these unmodded 12 qt shoeboxes. Lotta condensation recycling happening right now which I'm assuming is fine since they're not fully colonized but is this gonna be a problem in the near future? If it is what's the plan, flip the lid and observe? Punch some holes?

I’ve been wiping the undersides of the lids with paper towels so the drops don’t fall down and make the myc all matted or bruise the fruits. Holes would probably work too, but I’ve just been trying to stay the unmoddded course for now.

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Shop:

Isolated Cubensis Liquid Culture For Sale

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Liquid Cultures

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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