tryptkaloids said:

Ive done it for years.
No offence, but just because you fucked it up doesnt mean its not a valid way to seal cultures.

You messed up sterile technique which is okay and happens, but it wasnt the bag.

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root3r said:

tryptkaloids said:

Ive done it for years.
No offence, but just because you fucked it up doesnt mean its not a valid way to seal cultures.

You messed up sterile technique which is okay and happens, but it wasnt the bag.

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Yeah.  This makes me want to show my mistakes again.

FYI my initial 20 spore plates were pristine until I did my first transfer, requiring me to open the bag the new plates were in.  The outside of the bag was covered in contamination and it covered everything in the box very evenly as all can see.

But I just won't tell the truth next time.  Maybe some other noobs will make this same mistake.

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tryptkaloids said:

Ive done it for years.
No offence, but just because you fucked it up doesnt mean its not a valid way to seal cultures.

You messed up sterile technique which is okay and happens, but it wasnt the bag.

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root3r said:

tryptkaloids said:

Ive done it for years.
No offence, but just because you fucked it up doesnt mean its not a valid way to seal cultures.

You messed up sterile technique which is okay and happens, but it wasnt the bag.

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En mag ek net byvoeg, wat 'n onbeskaafd mens is jy, veral teenoor 'n nuweling.

Jy moet Amerikaans wees. En dit was die buitekant van die sak.

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root3r said:

I confess that I followed the 'bag your new plates' advice, and in removing my plates to innoc in the SAB it caused massiv infection of my most valuable culture, as well as many others which were open.


Don't do this.

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Smellyhobbit said:

You were the problem. It’s not the site, it’s you.

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fahtster said:

Just playing the devils advocate here… I’d think using bags with vented plates is probably a bad idea, no?  Kinda like how we don’t use a SAB with soft sides because any movement from contracting or expanding the sealed bag is going to shift all the air inside the bag, even through the vents?

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misterswisher said:

If you put them in a bag and let the bag get gross, didn’t even try to sanitize it, you’re gonna have issues. You cross contaminated your environment from an outside source and let a contamination in. It has nothing to do with the bag and everything to do with how you did it.

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fahtster said:

Just playing the devils advocate here… I’d think using bags with vented plates is probably a bad idea, no?  Kinda like how we don’t use a SAB with soft sides because any movement from contracting or expanding the sealed bag is going to shift all the air inside the bag, even through the vents?

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I would not trust it one bit, I have no problem leaving unwrapped plates in my SAB(with hole covers) for days, but I would never throw them in a ziplock and move that bag around without wrapping them, seems.



I use parafilm and it works wonders, I know some people get old parafilm that starts to crumble and is a PITA, I'm still on my first roll and I must have done +400 plates and probably wrapped them, at least, twice. I keep it in a ziplock and have not had a single issue thus far. I keep my unused plates in a 15qt tub and the inoculated ones in shoeboxes, I probably look and touch them every other day and wipe with ISO before loading it into the SAB, no issue there either.

GenesisCorrupted said:

Don't over analyze.
You asked a question and you found out there was a hole in your sterile technique.

That's great. Take it as progress that you've made. Don't take it as you trying to force conflict when there isn't any. Somebody helped you after you asked a question.

Everything after that is up to your interpretation.

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root3r said:

Don't worry, I get the picture.

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Uncivilized Americans

2 seconds later..

tryptkaloids said:

Like just stacked, unwrapped in a 15qt?

Call it what youd like, but it never caused a sattelite for me in years. Probably about 4 or 5 now

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No, the only place I keep unwrapped plates is in my SAB, when all condensation has cleared I wrap them in parafilm and then put them in a tub

And I have no problem believing you do this and do it without failure, but you really know your shit and have been doing it for years

I'm pretty sure that if I tried to use that technique I would fumble, stumble and fuck it up within an hour

Kloakk said:

And I have no problem believing you do this and do it without failure, but you really know your shit and have been doing it for years

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