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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: magicschoolbus] * 1
    #29262409 -

magicschoolbus said:

Sorry for the worlds shittiest picture but I got growth 48 hours later. The black spot is ofc a dark spore streak

Last attempt I had 3 jars have a couple white spots appear after 72 hours, although 12 hours later they turned green/grey.

This attempt is also with a different print from a grow i did about 2 years ago. Also using Malt/yeast instead of potato/karo

Anyways here's to hoping it stays white, so far this is the first visible growth out of the 12 jars I made. I anticipate there will be more colonies that pop up, but leaning towards going with the fastest



24 hours later, still not 100% sure if myc, kinda wish I didnt dye the agar now, hard to see anything through the jar. only 1 other jar has growth, about the size as the previous Pic, but 10 others do not have any visible growth


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:trippinballs::trippinballs:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 2
    #29262428 -

Thanks to all who gave advice. Much appreciated.
Thanks to all who gave advice. Much appreciated.


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Is not what we see or seem but a dream within a dream? -Edgar Allan Poe

:mushroom2:The Hitchhikers Guide to the Shroomery:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Harpo67]
    #29262548 -

Almost out of petri dishes. Thinking about using the small cups instead. Does anyone have a link of where to get the cups?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ClearLakeBoy] * 1
    #29263118 -

ClearLakeBoy said:
Almost out of petri dishes. Thinking about using the small cups instead. Does anyone have a link of where to get the cups?


Petris allow you to see what's going on much better than the cups.


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--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #29263142 -

nektar61 said:
ClearLakeBoy said:
Almost out of petri dishes. Thinking about using the small cups instead. Does anyone have a link of where to get the cups?


Petris allow you to see what's going on much better than the cups.


I just acquired some PP5 cups thinking I would be able to reuse them and they are prepour. They had so much condensation! I put them back in the SAB and used a paper towel to remove all the condensate and now hoping they aren't contaminated. I will be purchasing more regular petri dishes so I can see better as well. Just my 2 cents.


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Is not what we see or seem but a dream within a dream? -Edgar Allan Poe

:mushroom2:The Hitchhikers Guide to the Shroomery:mushroom2:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Harpo67] * 3
    #29263278 -

Unless your paper towels were sterilized, you def just ruined those cups.

I assume you're stacking them during sterilization? Use the bottom ones that dont get condensation and loosen the lids on the others and let them dry.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ThurmanMurman]
    #29263320 -

ThurmanMurman said:
Unless your paper towels were sterilized, you def just ruined those cups.

I assume you're stacking them during sterilization? Use the bottom ones that dont get condensation and loosen the lids on the others and let them dry.


:whathesaid:

Even with sterilized paper towel there is still a chance for contamination.


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The Hitchhikers Guide to the Shroomery
:sporedrop:L.A.G.M. 2025:sporedrop:
Tico


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Axman] * 1
    #29263411 -

Thanks, Petris it is


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ClearLakeBoy] * 3
    #29263426 -

Got my transfers taken care of :mushroom2:


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Island clean up
Pasteurizing Straw with Hydrated Lime
Swiss Cheese Fruiting Chamber

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ClearLakeBoy] * 1
    #29263434 -

ClearLakeBoy said:
Thanks, Petris it is


Check out Rev’s Primo Plates tek - reusable petri is awesome.


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The Hitchhiker’s Guide to the Shroomery - BeefSupremeJr
A Love Letter to New Growers - Smellyhobbit

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nocap] * 6
    #29263454 -





Backlit agar plates make my dick hard 😩


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Face failure head on and learn something from it
The Hitchhiker's Guide to the Shroomery

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Harpo67]
    #29263699 -

Harpo67 said:
nektar61 said:
ClearLakeBoy said:
Almost out of petri dishes. Thinking about using the small cups instead. Does anyone have a link of where to get the cups?


Petris allow you to see what's going on much better than the cups.


I just acquired some PP5 cups thinking I would be able to reuse them and they are prepour. They had so much condensation! I put them back in the SAB and used a paper towel to remove all the condensate and now hoping they aren't contaminated. I will be purchasing more regular petri dishes so I can see better as well. Just my 2 cents.


Condensation is from pouring too hot and happens to regular petris too


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #29263908 -

My agar plates are always lack luster. These are all T3s from spore, with the acception of a spore plate in pic I think 5. Is it just a matter of making more transfers until I get rhizo growth?











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I eat a few.  should be real tender.  Its tasty.  No starchiness or crunchiness.

Edited by el boso (06/29/25 02:38 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: el boso] * 3
    #29263917 -

Are these all cubes? A lot of other species mycelium will not express rhizo at all.

As for cubes, it depends, they can switch expression on the plate, they might go rhizo if you transfer to a low nutrient plate or a higher nutrient plate or if you use a different nutrient source i.e. you transfer from MEA to PDA.

Rhizo does not really mean anything except that a culture expresses rhizo and it's easier to distinguish mushroom mycelium from mold mycelium but that is about it.

You can grow canopies of potent mushrooms from either tomentose or rhizomorphic, or cultures that express as both!

What's important is that the culture is clean :rockon:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dkeppel]
    #29263939 -

EPIC!

I did throw a pan, tamp, sub and semp in there.
So all cubes express rhizo, pans don't. Do other Ps species express rhizo?


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I eat a few.  should be real tender.  Its tasty.  No starchiness or crunchiness.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: el boso] * 1
    #29263946 -

All cubes can express rhizo, depending on the genetics some varieties are more likely to than others.

Pans, tamps (section mexicanae) and semps (section zapotecorum) will not but subs (section cyanescens) can.

Nothing in the Panaeolus genus will express rhizomorphic and as for the Psiocybe genus I think species in section aztecorum, caerulescens, cubensae and cyanescens all can but I am not sure if it's all species in those sections, and maybe I missed a section?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dkeppel]
    #29263953 -

Epic. I feel like my mycelium is hella wispy and too transparent. Maybe I'm paranoid maybe I have contam. Either way I might have a protocol where I put all my cultures through a acbin sequestering procedure.

When looking at those photos, can you, as an experienced cultivator tell what areas I should be taking for T4s?

Thanks for your helps as usual I


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I eat a few.  should be real tender.  Its tasty.  No starchiness or crunchiness.

Edited by el boso (06/29/25 05:59 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: el boso] * 3
    #29263964 -

Whatever the species I'm working with, I'm looking for fast organised growth with a clearly defined leading edge, assuming you want fruit and not sclerotia when working with species in section mexicanae, I will avoid the mycelium with a pigment and select for the white stuff.

It's a little difficult when I don't know exactly which species each plate is and from only a single photo without a backlight but I will do my best :lol:

Plate 1:

I am assuming this is a cube.

If those spots are sediment, you could probably let this culture to continue to grow out and send it to grain, assuming it continues to grow organised with a clearly defined leading edge.

If you want to transfer, everything from about 1 to 8 o'clock looks good.


Plate 2:

This looks like semps? Or is it the Pan you mentioned?

It's really hard to see what's going on but I think the bottom culture looks better, 11 to 4 o'clock, hold it up to a back light and check the growth is organised etc.


Plate 3:

Assuming semps again? Maybe this is a pan?

I don't like how it's growing, possibly has bacteria riding along, if you want to try save it, give cabin sequesting a try, growth at about 9 o'clock looks the best I think.


Plate 4:

Looks section zapotecorum. Semps :lol:

Looks like you have an orange dot close to the transfer, 7 to 11 o'clock looks like the nicest growth.


Plate 5:

This looks like your germination plate so I will assume I should ignore this one.


Plate 6:

Maybe this one is the sub?

This looks sketchy, you might want to give cabin sequestering a try, looks like some nice growth between 12 to 1 o'clock and about 8 to 9 o'clock.


Plate 7:

These next plates all look section mexicanae so I'll assume they are tampanensis since you mentioned tamps.

Top culture, I cant see what's under the glad wrap but I don't like much of what I can see, maybe around the 3 o'clock region.

Right-hand culture, looks like 4 to 5 o'clock has some nice growth.

Bottom culture, this is the best looking culture on the plate, 4 to 10 o'clock section looks good.

Left-hand culture, section between 8 to 9 o'clock.


Plate 8:

Looks like another possible bacteria riding along culture, again cabin sequesting if you want to try save it, about 10 o'clock I think.


Plate 9:

Again, it is hard to see what is going on where the glad wrap is covering the mycelium but these all look clean from here.

Maybe let them continue to grow out and if you feel the need to transfer, again hold it up to a back light, look for the fastest organised growth with a clearly defined leading edge and transfer from there.


I hope that helps! Good luck.

Edited by Dkeppel (06/29/25 07:05 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dkeppel]
    #29263970 -

Holy beast! Thanks for doing all that, I was just looking for some Tek advice but you gave me the full baby girl treatment. Really appreciate it!


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I eat a few.  should be real tender.  Its tasty.  No starchiness or crunchiness.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: el boso]
    #29263971 -

My bad :facepalm3:

I look at a lot of mycelium so I took it as an unlabelled plates challenge.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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