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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 2
    #29251231 -

You've done all this without SAB?

I've seen someone just use a clear tote(unmodified) on a tall dresser overhanging the edge just enough to get their Arms in to do the work. Doesn't seem the most comfortable, but worked.

Edited by LewDoja (06/15/25 01:36 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Home Grower]
    #29251254 -

yes its starting to knot

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29251257 -

That could be something to look into for sure regarding the tote.

But yeah. I’ve never had a SAB. I will use my instapot for sterilize. Wipe down all my counters with 70% and some Lysol (I’m a clean freak with my bathroom already). No air gets blown into my BR and it’s the master bath of a 3k sqft house.

With the hundreds of grains I’ve done I’ve only lost a couple so well within the margin for error. With the one complete batch I lost, it was all the same syringe and I turned around and did everything the same way for 3 more grows with no issues.

My bigger problems tend to be once I get to the bulk portion and I don’t know how to leave it the fuck alone.

Trying my hands at agar, I’m not going to be against SAB clearly, but that’s something after this attempt it if I start beating my head into the wall will happen for sure.

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs] * 3
    #29251274 -

Splittinghairs said:
Dr. Smoke said:
6/7 is about the same size as 6/5 so seems faster.

But I think I see contam in all of them.  Hard to tell with the reflections and blue agar.  Are you sterilizing for an hour?  Letting the air out of the PC?  Using an SAB?



Yeah. I used the insta tech and let them sterilize for an extra half hour.

I use my over sized bathroom for sterilized techs like grains and have only lost a couple of jars over all my grows (not including one syringe where I lost all the jars cause assuming that syringe was bunk)

Non of the 50 plates I have made started growing anything unless I’ve put a swab to it. All my reserve plates are still clean so I don’t think I introduced contam during the pour.

There is contam on the plates that have myc growth but assuming that’s from swabs.

As far as the white patches they shine through with light and no discoloration. The 6/7 one you pointed out I will probably transfer some later as on the other side of the dish there is contam as well.

Unless I’m not understanding agar, I thought contam from swabs happens as it’s part of the process from wiping?

It’s a shame. Cause one of my TW plates just got some white growth, but I can see a pink dot in the center.

Is there a better color to use other than blue?



NO color.

In any room you Earthlings inhabit there is always Evil floating down from above.

And everyone does their own thing but I would never use swabs.  You're just asking for it.

I use a SAB and innoc loop.  I flame the loop with a jet lighter (weed shop) to red-hot before every transfer, cool it in agar, and only open the petri long enough to do the transfer from print, even though I'm in the SAB.  And Beef says never move your hands over an open dish.

Most here bought at Goodwill, for cheep:

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Re: Ask Quick Questions, Get Quick Answers [Re: Dr. Smoke]
    #29251290 -

Gonna have to look up the innoc loop stuff.

I just picked one method and went with it. (Being swabs)

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Re: Ask Quick Questions, Get Quick Answers [Re: dinosaurs]
    #29251291 -

dinosaurs said:
yes its starting to knot



How long do you think before I have pins?


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29251292 -

Splittinghairs said:
Gonna have to look up the innoc loop stuff.

I just picked one method and went with it. (Being swabs)



I just use a scalple, flame sterilize, and cool in fresh agar plate.

I know some use loops here, but they were adopted here from biolabs, and seem more suited to sticky things like bacteria cultures than powders like spores.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29251295 -

Splittinghairs said:
That could be something to look into for sure regarding the tote.

But yeah. I’ve never had a SAB. I will use my instapot for sterilize. Wipe down all my counters with 70% and some Lysol 




you do shroom work with agar but no SAB, and have done lots of grows with no contam, are you sure no contam?

bacteria can produce not a lot visually, can usually be smelled, but maybe you don't know what to look for. Jars not completing 100 percent within a month is one example (and not completing even if you go two more weeks), spawn in jar being a hard ball that doesn't want to come out of jar is another.

Doing this right with no still box of some kind (or flow hood), seems shocking that it would work. Especially with insta pot not PC.

got pix of your work and results? Do you flame scalpel? how'd you learn to grow, you've done hundreds of grows but joined here this year.

Not saying this is the only path but I'm still incredulous. Your tagline says "know it all", and you posted yesterday that you recently did your first ever agar. And that's mold on at 3 oclock on that plate, the dark spot.

https://www.shroomery.org/forums/showflat.php/Number/29240121




--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (06/15/25 02:57 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs] * 3
    #29251300 -

Splittinghairs said:
Gonna have to look up the innoc loop stuff.

I just picked one method and went with it. (Being swabs)



Swabs are fine. They're all I've used over the years. Your initial impression (that ridealongs are just going to happen) is correct. Whether you use a scalpel, swab, or loop, a print is what it is (clean/dirty/meh) & if there are nasties on it, they will come along for the ride. Blue is fine. You're doing great so far, keep it up.


--------------------
CV+

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Re: Ask Quick Questions, Get Quick Answers [Re: Home Grower]
    #29251304 -

Home Grower said:
How long do you think before I have pins?



Patience, Grasshopper.

Less than a week. What's the variety and how warm is it? (do NOT add heating pads, that brings contam)


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] * 1
    #29251305 -

True, most here use scalpels for transfer.  Some prints are stuck fast, depending on age.  It may be that a scalpel is better. (Feed & Seed store)

Contam on the underside is a sure sign that you are not achieving sterilization, FYI.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61]
    #29251316 -

nektar61 said:
Splittinghairs said:
That could be something to look into for sure regarding the tote.

But yeah. I’ve never had a SAB. I will use my instapot for sterilize. Wipe down all my counters with 70% and some Lysol 




you do shroom work with agar but no SAB, and have done lots of grows with no contam, are you sure no contam?

bacteria can produce not a lot visually, can usually be smelled, but maybe you don't know what to look for. Jars not completing 100 percent within a month is one example (and not completing even if you go two more weeks), spawn in jar being a hard ball that doesn't want to come out of jar is another.

Doing this right with no still box of some kind (or flow hood), seems shocking that it would work. Especially with insta pot not PC.

got pix of your work and results? Do you flame scalpel? how'd you learn to grow, you've done hundreds of grows but joined here this year.

Not saying this is the only path but I'm still incredulous. Your tagline says "know it all", and you posted yesterday that you recently did your first ever agar. And that's mold on at 3 oclock on that plate, the dark spot.

https://www.shroomery.org/forums/showflat.php/Number/29240121






Wait wait wait.

Not sure where the know it all tagline thing is but that’s not something I have in mine that I put in for sure. If I knew it all the last place I would be in most of the time is in the questions areas trying to fine tune things and understand. So that’s a huge misunderstanding somewhere.

With regards to hundreds. It’s jars. I’ve gone through a hundred grain jars. I have journals that have pics of grows. There are other websites that people talk about growing that I USED to be at. So the being here isn’t the only window of me growing. But I left the other websites cause I didn’t see a way to learn more there as information often regurgitated there was bad/old stuck in archaic ways. So I joined here to learn some more.

The old tech I used before am not allowed to mention as it’s one of the more popular techs on the video platform and thus, incredibly shitted on here. Name of a USA city and someone who you learn from.

Yes I flamed my scalpe before making a cut AFTER I wiped everything down with ISO. I let it sit for a couple of minutes before making an incision and side poked what I cut to move the transfer over to the other plate and didn’t move my hands back and forth over the plate.

I’ve had some contam in my jars. But I’m talking like 1 for every 7 or 8 which to me falls within more than acceptable lines. What I spawn should be

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Re: Ask Quick Questions, Get Quick Answers [Re: Dr. Smoke]
    #29251319 -

Dr. Smoke said:
True, most here use scalpels for transfer.  Some prints are stuck fast, depending on age.  It may be that a scalpel is better. (Feed & Seed store)

Contam on the underside is a sure sign that you are not achieving sterilization, FYI.



That could be from pouring in open air... otherwise the rest would show signs...


Edit:
@splitting

You've done great this far and surprisingly so... are you making your own swabs/prints?

I've noticed that the 2nd swab/prints are usually more contam free.
....i.e let cap drop for a few hours--> carefully transfer to another piece of foil.
It will be faint/lighter but cleaner.

Same with swabs (although best to take inside SAB)...
It's about minimizing exposure to environment, but swabing/rolling the tip in the Same place as the one before will be cleaner (should be).

The goal is to not need to clean up and everything is much smoother, but yes agar is used to acquire an clean culture, though not it's only purpose.

You will definitely be able to level-up drastically if you implement a SAB

Edited by LewDoja (06/15/25 03:34 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: MojaveMyc]
    #29251326 -

MojaveMyc said:
Splittinghairs said:
Gonna have to look up the innoc loop stuff.

I just picked one method and went with it. (Being swabs)



Swabs are fine. They're all I've used over the years. Your initial impression (that ridealongs are just going to happen) is correct. Whether you use a scalpel, swab, or loop, a print is what it is (clean/dirty/meh) & if there are nasties on it, they will come along for the ride. Blue is fine. You're doing great so far, keep it up.



Ok. I was under the impression that part of the agar process was getting the shit out of the swabs so transferring was going to be apart of the process.

If my plates that I poured and are untouched don’t show any growth then they would be clean correct?

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29251328 -

Good point Lew, but he does have top-surface contam in all three that he showed.

If untouched but clean, what about the underside contam?

I never take the lid off except minimally to pour and minimally to innoc.  I treat the first two stages (agar, grain) as I would surgery.

Edited by Dr. Smoke (06/15/25 03:27 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29251329 -

Guys my CRS had a bad case of CRS.  Would an initial cross be F0 or F1?  Been a minute since I've used the terminology.


-Kingboomer


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

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Re: Ask Quick Questions, Get Quick Answers [Re: kingboomer] * 1
    #29251330 -

F1 is your first "produced" spores ....
So F0

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29251333 -

Splittinghairs said:
Wait wait wait.

Not sure where the know it all tagline thing is but that’s not something I have in mine that I put in for sure. If I knew it all the last place I would be in most of the time is in the questions areas trying to fine tune things and understand. So that’s a huge misunderstanding somewhere.

With regards to hundreds. It’s jars. I’ve gone through a hundred grain jars. I have journals that have pics of grows. There are other websites that people talk about growing that I USED to be at. So the being here isn’t the only window of me growing. But I left the other websites cause I didn’t see a way to learn more there as information often regurgitated there was bad/old stuck in archaic ways. So I joined here to learn some more.

The old tech I used before am not allowed to mention as it’s one of the more popular techs on the video platform and thus, incredibly shitted on here. Name of a USA city and someone who you learn from.

Yes I flamed my scalpe before making a cut AFTER I wiped everything down with ISO. I let it sit for a couple of minutes before making an incision and side poked what I cut to move the transfer over to the other plate and didn’t move my hands back and forth over the plate.

I’ve had some contam in my jars. But I’m talking like 1 for every 7 or 8 which to me falls within more than acceptable lines. What I spawn should be



I'm half kidding with pointing out the "know it all" but you don't know it? It's on your user page:
https://www.shroomery.org/forums/showprofile.php?User=630963

"Bio: Parent veteran construction worker and know it all"

I think you should make an SAB. I'm not trying to shame you, I'm trying to help you grow clean, tasty shrooms.

I recommend you post those 3 APE plates here on this thread and on the Agar Envy thread, and ask for second opinions. I'm seeing mold on two of them, without even looking big and close.

You could easily transfer away the good there to new plates and have really clean spawn in 1 or 2 transfers with an SAB, but I believe it's never going to happen in open air, no matter how much Lysol you use. (

God I can smell that crap in my mind from my first month here just at the mention of it. I still have a half-unused can I haven't touched since.

Read my sig line link, (FROM SPORES TO TRIPS). It's tips, and link to a good SAB tek.

You can get an amazingly good pressure cooker for 110 bucks on Amazon, and I give the model number there.

A few people here have managed to do clean work with an insta pot, long cook times, and an SAB. I don't think anyone has done it without the SAB.

An SAB costs 10 bucks to make and takes 20 minutes to make. You say you'll do it if you get contam, but you have contam.

I'd also recommend not using colored agar until you can tell when you have contam.

Welcome.

Is the "he who cannot be named" on YouTube the idiot with the skull mask? We mention him and make fun of him all the time, it's not forbidden. A lot of us spend a lot of fucking energy and time for free undoing the damage he does, while he gets rich off his Patreon.

:yields:


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (06/15/25 04:42 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 2
    #29251335 -

LewDoja said:
F1 is your first "produced" spores ....
So F0




Ok cool, so the prints from those fruits would be F1 then.  Many thanks!


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29251340 -

LewDoja said:
F1 is your first "produced" spores ....
So F0



"What's T1, T2, T3 etc. And what's F1, F2, F3?"


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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