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Re: Official Copelandia/Panaeolus Thread [Re: rhizoRider] * 1
    #29139720 -

They’re so cool Rhizo! Get to clone any?

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Re: Official Copelandia/Panaeolus Thread [Re: 3.A.M] * 2
    #29139838 -

Ok my original plan is out :lol:


I have brown rice, verm, popcorn, wheat and 55lbs of fertilizer so I guess I'll have to try some different things and pin down what bisporus prefers to colonize in a decent amount of time before I can move on to ez dial bulk then.

I think I'll try a couple of different things:
- The brf/verm grain top layer.
- Fertilizer infused popcorn
- Fertilizer infused wheat
- Popcorn

Have you found at what NPK they do best?

Again, thank you :hatsoff:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Fantastic Mr. Fox] * 9
    #29139854 -

Chonker begets chonkers- when they have the right environment. I cloned a chubby from an F2 MIB print (thanks sleepydave)>agar>T1>muddafukka 8 oz deli dish. Same clone plate T1provided li for a bunch of EF jars. A couple wouldn’t birth so I left them in the jar to fruit. I never would’ve guessed they came from the same clone. I printed 2 chubby caps for posterity.
Not only are they chubby they also kick ass. 3 g fresh in a teabag, steeped in hot coffee was a great joyride with no stomach symptoms. So much fun!





Here they are side by side. They need room to grow!


Edited by Imatryin (02/27/25 12:44 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: Imatryin] * 6
    #29139928 -

So my first shoeboxes of Pan Cyan. I thought liners were supposed to PREVENT sidepinning.


Edited by DV8DUG (02/27/25 04:21 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29139946 -

tree frog said:
I'm not really sure why it doesn't colonize grains well.  Possibly, they struggle to produce the enzymes to penetrate grain hulls easily.




Okay, let me see if I got this right. So the hyphae secrete enzymes onto or into the food source. The enzymes break down carbohydrates and proteins, which are then absorbed through the walls of the hyphae.

In layman terms it uses enzymes to break down complex sugars(3 or more sugar units that are attached together via long and/or branched chains.) to simple sugars(1 and 2 sugars units).

If bisporus is struggling to produce digestive enzymes on most grains, then grains are lacking or have too much of one or more "ingredient(s)". Wheat is my preferred grain, has anyone tried bisporus on wheat?

And if it does thrive on popcorn, then the question is, what does popcorn have or don't have, that most grains do or don't? Well, Zea mays are cultivated specifically as popping corns. The Zea mays variety everta, a special kind of flint corn, is the most common of these so I'll assume ladyfinger is flint corn. Hulless apparently only means small kernel. Popcorn have a core of soft, wet endosperm surrounded by a layer of hard, stony endosperm.

Even though my wheat is unmalted it is cultivated for the purpose of brewing beer, that would imply that it is a soft wheat and I can't see why it should be more of a challenge for bisporus to break down wheat vs popcorn, or?


As far as I can see, there isn't any concerning vitamins or minerals values in wheat compared to popcorn, so what then, is it that bisporus is lacking or not liking about other grains? Popcorn has 30% more zinc, then again wheat has 50% more nitrogen, how much of that is bioavailable to the mycelium I don't know and can't find anything about..

Sorry for just thinking out loud here, but there's got to be a reason bisporus is stalling on most grains.. :wow:

When you add fertilizer to your grain prep, do you rinse with water after boil?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 2
    #29140013 -

I don't know about bioavailability for bisporus.  But for people, wheat, brown rice, and corn have more nitrogen than any other commonly consumed grains.

I've got it to run well on both corn and brown rice without adding nitrogen to either to get it to run on them.  Though I did add it to my substrate in every case.  I never ran a non-supplemented control all the way to fruiting.

I don't know the most nitrogen they like.  I think my liquid ferts were 0.3 seemed preferable.  For subs, when not using liquid fertilizer, I found fifty percent cow manure good.  If using horse I would use less Jake used 30% cow manure rest mostly stray in his JOC tek.  But the stuff I use is a commercial bag that's very well composted and has lots of plant matter anyway.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29140066 -

:takingnotes:

I see, just did 7 transfers from T0 to T1's. While I wait for them to grow out on the plate I'll draw a new plan and try a couple of different things when they are ready for grains. Speaking of that, do they stall on agar too? How much growth before inoculate jars?

And sorry for nagging, but do you rinse the fertilize infused popcorn after boil, I am asking because I think it's relevant to what is available to the mycelium.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 5
    #29140077 -

I did rinse my popcorn but you probably don't need to. I've both rinsed it and not rinsed it.  But I did rinse it the time I ran it with liquid fertilizer, but I used to think that rinsing it would help it not clump but it just doesn't clump really. It's almost more gelatinous than sticky.  Well, you've had canned corn.

If you use 0.5% nutrient agar it shouldn't stall.

If I can get a good plate shot I'll post one in a bit in an edit.

Also, no need to apologize :smile:  If I didn't enjoy helping people and answering questions, I wouldn't do it :wink:

Edit: 0.5% lme








--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (02/27/25 09:36 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 4
    #29140124 -

Nice, well if you rinsed and it still did good I'll rinse, it's what I usually do when prepping grains anyway and if a nutrient surface isn't what matters then I don't need to change my grain prep flow :biggrin:

Yes I'm using 0,5% DME for pan plates. And thanks for answering, I am mostly apologizing because I tend to get carried away when trying to understand things, and end up asking alot of questions in short time, but noted and won't feel bad for asking going forward :awesomenod:

I've read a couple of stonesun's threads and he seems to have fast growth with rye berries, only a week for a qt jar :eek: I'll play around with wheat and popcorn, and see what happens.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: rhizoRider] * 3
    #29140583 -

rhizoRider said:
Hey enthusiasts :trippinbawelz:

Finally found wild pan cyans in phillipines cebu island mountains :awedance::wooyeah:

Also found more purple pink, blue bruising gyms on same logs as last may.








What a wonderful find! It would be amazing if you could print, or clone, and share the genetics. I can't remember anyone here growing the shrooms from the Philippines, at least, it's not very popular to grow anything from this part of the world.
It would be great if this genetics could stay among the shroomers, as it did with Burringbar or BVI, for example. Landrace shrooms have their own personality. I can feel it with every cell and sense, there is something special about them.


Burringbar





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Re: Official Copelandia/Panaeolus Thread [Re: Bra] * 5
    #29140616 -

I’ve had no problem running bisporus on wheat.

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog] * 1
    #29140621 -

Quote:
  Bispo likes to stall out on most grains




put some bisporus on wbs last week. saw this post now :rolleyes:


--------------------
Learn it, know it, live it


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Re: Official Copelandia/Panaeolus Thread [Re: RoscoeReturns] * 4
    #29140624 -

I have some bispo on plates and just prepped some millet, wheat, rye jars that came out nice.

I'll try a wedge inoculation with it and then once I have the culture in LI, hit it with that if it hasn't taken off yet.

When I grew it with wheatgrass in the casing, it seemed to like to colonize the germinated seeds iirc

And I may have been overstating Mackload, it stalled on millet and rye for me and when I asked about it other folks , but not everybody, said they had had issues with pans generally on some grains.  I think prep and inoculation method may have as much to do with it as grain choice.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Official Copelandia/Panaeolus Thread [Re: Mackload] * 1
    #29140726 -

@mackload, I use wbs, Wally's economy!  Seems to do well for me, using it for a bit now. But I've never really compared against an aio.

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Re: Official Copelandia/Panaeolus Thread [Re: stagger] * 5
    #29140778 -

So what amount of fresh Burringbar have you guys tried? I have a window to journey tomorrow. The wife and kid went on vacation for a few days, just a couple of cats around.

Torn between Bbar fresh or some dry subtrops which I haven't tried yet either :dancingshroom:

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 5
    #29140800 -

Baba Yaga said:
So what amount of fresh Burringbar have you guys tried? I have a window to journey tomorrow. The wife and kid went on vacation for a few days, just a couple of cats around.

Torn between Bbar fresh or some dry subtrops which I haven't tried yet either :dancingshroom:



Yes

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 7
    #29140836 -

OK, there won't be an either or..... I'm going to mix it up.

Here is my preliminary trip report:

:anticipation::sunsalutation::mindblown::singletear::sosleepy::sleep:

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga] * 1
    #29140943 -

ahah Baba that gifgame is so on point!
and that yawning cat sent me to linkedin!?
:utterconfusion:


--------------------
“All this buttoning and unbuttoning”

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Re: Official Copelandia/Panaeolus Thread [Re: tree frog]
    #29140967 -

RoscoeReturns said:
I’ve had no problem running bisporus on wheat.



This is great news, do you mind sharing your wheat prep?

tree frog said:
I have some bispo on plates and just prepped some millet, wheat, rye jars that came out nice.

I'll try a wedge inoculation with it and then once I have the culture in LI, hit it with that if it hasn't taken off yet.

And I may have been overstating Mackload, it stalled on millet and rye for me and when I asked about it other folks , but not everybody, said they had had issues with pans generally on some grains.  I think prep and inoculation method may have as much to do with it as grain choice.



Cool, excited to see how it goes :strokebeard:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 2
    #29141089 -

https://www.shroomery.org/forums/showflat.php/Number/25546266

Here's a grow log where someone used black cow straight out of the bag. I've been thinking this stuff was already heat treated, and I think I just messed a batch up by trying to he treat it and lost a few grows because of it.

I have some projects on plates, after they colonize some jars I will give it a try as I already have a bag and a half here.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
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Shop: North Spore Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Autoflowering Cannabis Seeds   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags


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